{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,16]],"date-time":"2026-03-16T11:53:22Z","timestamp":1773662002001,"version":"3.50.1"},"reference-count":27,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2005,2,24]],"date-time":"2005-02-24T00:00:00Z","timestamp":1109203200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics"],"published-print":{"date-parts":[[2005,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Strategies employing non\u2010gel based methods for quantitative proteomic profiling such as isotope coded affinity tags coupled with mass spectrometry (ICAT\u2010MS) are gaining attention as alternatives to two\u2010dimensional gel electrophoresis (2\u2010DE). We have conducted a large\u2010scale investigation to determine the degree of reproducibility and depth of proteome coverage of a typical ICAT\u2010MS experiment by measuring protein changes in <jats:italic>Escherichia coli<\/jats:italic> treated with triclosan, an inhibitor of fatty acid biosynthesis. The entire ICAT\u2010MS experiment was conducted on four independent occasions where more than 24\u2009000 peptides were quantitated using an ion\u2010trap mass spectrometer. Our results demonstrated that quantitatively, the technique provided good reproducibility (median coefficient of variation of ratios was 18.6%), and on average identified more than 450 unique proteins <jats:italic>per<\/jats:italic> experiment. However, the method was strongly biased to detect acidic proteins (p<jats:italic>I<\/jats:italic> &lt; 7), under\u2010represented small proteins (&lt;10\u00a0kDa) and failed to show clear superiority over 2\u2010DE methods in monitoring hydrophobic proteins from cell lysates.<\/jats:p>","DOI":"10.1002\/pmic.200400994","type":"journal-article","created":{"date-parts":[[2005,2,24]],"date-time":"2005-02-24T10:53:45Z","timestamp":1109242425000},"page":"1204-1208","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":46,"title":["Large\u2010scale evaluation of quantitative reproducibility and proteome coverage using acid cleavable isotope coded affinity tag mass spectrometry for proteomic profiling"],"prefix":"10.1002","volume":"5","author":[{"given":"Mark P.","family":"Molloy","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sam","family":"Donohoe","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Erin E.","family":"Brzezinski","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Greg W.","family":"Kilby","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tracy I.","family":"Stevenson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. David","family":"Baker","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David R.","family":"Goodlett","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Douglas A.","family":"Gage","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,3,31]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1002\/1615-9861(200201)2:1<3::AID-PROT3>3.0.CO;2-R"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1367-5931(01)00275-7"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0958-1669(02)00333-6"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2836(82)90515-0"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.160270797"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/elps.1150190847"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1522-2683(20000401)21:6<1054::AID-ELPS1054>3.0.CO;2-8"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1002\/1615-9861(200207)2:7<899::AID-PROT899>3.0.CO;2-Y"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1038\/13690"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1038\/nbt1001-946"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0958-1669(02)00018-6"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.M300021-MCP200"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.119.3.736-747.1974"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.16.11110"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.1150"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.19.3.1720"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac025747h"},{"key":"e_1_2_1_19_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac0341261"},{"key":"e_1_2_1_20_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac034633i"},{"key":"e_1_2_1_21_2","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200300534"},{"key":"e_1_2_1_22_2","doi-asserted-by":"publisher","DOI":"10.1002\/elps.11501601320"},{"key":"e_1_2_1_23_2","doi-asserted-by":"publisher","DOI":"10.1002\/1522-2683(200207)23:14<2203::AID-ELPS2203>3.0.CO;2-H"},{"key":"e_1_2_1_24_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac010583a"},{"key":"e_1_2_1_25_2","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.M300074-MCP200"},{"key":"e_1_2_1_26_2","doi-asserted-by":"publisher","DOI":"10.1038\/85686"},{"key":"e_1_2_1_27_2","doi-asserted-by":"publisher","DOI":"10.1038\/nbt819"},{"key":"e_1_2_1_28_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac034120b"}],"container-title":["PROTEOMICS"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fpmic.200400994","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/pmic.200400994","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T14:27:01Z","timestamp":1761056821000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/pmic.200400994"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005,3,31]]},"references-count":27,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2005,4]]}},"alternative-id":["10.1002\/pmic.200400994"],"URL":"https:\/\/doi.org\/10.1002\/pmic.200400994","archive":["Portico"],"relation":{},"ISSN":["1615-9853","1615-9861"],"issn-type":[{"value":"1615-9853","type":"print"},{"value":"1615-9861","type":"electronic"}],"subject":[],"published":{"date-parts":[[2005,3,31]]},"assertion":[{"value":"2004-06-01","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2005-03-31","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}