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Laser capture microdissection (LCM) may allow a more ready identification of differences in protein expression in selected cell types or areas of tissue, and microscopic regions as small as 3\u20135\u2005\u03bcm in diameter can be sampled. Here we applied the LCM to the proteomic study of hepatitis B\u2010related HCC and surrounding non\u2010tumor tissues. Proteome alterations were observed using 2\u2010DE and ESI\u2010MS\/MS, and alterations in the proteome were examined. Twenty protein spots were selected, of which 11 proteins were significantly altered in the HCC compared with the surrounding non\u2010tumor tissues. Of the proteins that were selected, peroxiredoxin 2, apolipoprotein A\u2010I precursor, 3\u2010hydroxyacyl\u2010CoA dehydrogenase type II, and 14.5\u2010kDa translational inhibitor protein appear to be novel candidates as useful hepatitis B\u2010related HCC markers. This study indicates that LCM is a useful technological method in the proteomic study of cancer tissue. The proteins revealed in this experiment can be used in the future for studies pertaining to hepatocarcinogenesis, or as diagnostic markers and therapeutic targets for HCC associated with hepatitis B virus infection.<\/jats:p>","DOI":"10.1002\/pmic.200500257","type":"journal-article","created":{"date-parts":[[2005,12,9]],"date-time":"2005-12-09T09:46:58Z","timestamp":1134121618000},"page":"538-546","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":61,"title":["Proteome analysis of hepatocellular carcinoma by laser capture microdissection"],"prefix":"10.1002","volume":"6","author":[{"given":"Jianhua","family":"Ai","sequence":"first","affiliation":[]},{"given":"Yexiong","family":"Tan","sequence":"additional","affiliation":[]},{"given":"Wantao","family":"Ying","sequence":"additional","affiliation":[]},{"given":"Yi","family":"Hong","sequence":"additional","affiliation":[]},{"given":"Shuqing","family":"Liu","sequence":"additional","affiliation":[]},{"given":"Mengchao","family":"Wu","sequence":"additional","affiliation":[]},{"given":"Xiaohong","family":"Qian","sequence":"additional","affiliation":[]},{"given":"Hongyang","family":"Wang","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2006,1,17]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0959-8049(01)00267-2"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1006\/scbi.1998.0079"},{"key":"e_1_2_1_4_2","first-page":"1","volume":"3","author":"Harris C. 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