{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T05:24:43Z","timestamp":1789190683269,"version":"build-2803163510"},"reference-count":63,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2006,2,27]],"date-time":"2006-02-27T00:00:00Z","timestamp":1140998400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics"],"published-print":{"date-parts":[[2006,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Human colostrum is an important source of protective, nutritional and developmental factors for the newborn. We have investigated the low abundance proteins in the aqueous phase of human colostrum, after depletion of the major proteins secretory IgA, lactoferrin, alpha\u2010lactalbumin and HSA by immunoabsorption, using 2\u2010D LC and gel\u2010based proteomic methods. One hundred and fifty\u2010one proteins were identified, 83 of which have not been previously reported in human colostrum, or milk. This is the first comprehensive proteomic analysis of human colostrum produced during the first 48\u2005h of lactation.<\/jats:p>","DOI":"10.1002\/pmic.200500558","type":"journal-article","created":{"date-parts":[[2006,2,27]],"date-time":"2006-02-27T05:18:25Z","timestamp":1141017505000},"page":"2208-2216","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":85,"title":["Human colostrum: Identification of minor proteins in the aqueous phase by proteomics"],"prefix":"10.1002","volume":"6","author":[{"given":"David J.","family":"Palmer","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Van C.","family":"Kelly","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne\u2010Marie","family":"Smit","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sulee","family":"Kuy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catriona G.","family":"Knight","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Garth J.","family":"Cooper","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2006,3,22]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1002\/elps.1150191427"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/elps.1150100117"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1515\/jpme.1986.14.4.251"},{"key":"e_1_2_1_5_2","first-page":"303","volume":"49","author":"Cavaletto M.","year":"1994","journal-title":"Milchwissenschaft"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.idairyj.2004.04.004"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200300367"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/10890"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/85686"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac034869m"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(85)90442-7"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/16.11.1048"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac026122m"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.0014-2956.2001.02666.x"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1651-2227.1992.tb12184.x"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1440-1711.2001.01013.x"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1203\/00006450-199907000-00013"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi026513v"},{"issue":"1","key":"e_1_2_1_19_2","doi-asserted-by":"crossref","first-page":"S119","DOI":"10.1017\/S0007114500002348","volume":"84","author":"Groziak S. M.","year":"2000","journal-title":"Br. J. Nutr."},{"key":"e_1_2_1_20_2","doi-asserted-by":"publisher","DOI":"10.1038\/ni1113"},{"key":"e_1_2_1_21_2","doi-asserted-by":"publisher","DOI":"10.1136\/vr.154.12.361"},{"key":"e_1_2_1_22_2","first-page":"275","volume":"133","author":"Ulcova\u2010Gallova Z.","year":"1994","journal-title":"Cas. Lek. Cesk."},{"key":"e_1_2_1_23_2","first-page":"85","volume":"78","author":"Ladjeva I.","year":"1989","journal-title":"Clin. Exp. Immunol."},{"key":"e_1_2_1_24_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1651-2227.1992.tb12185.x"},{"key":"e_1_2_1_25_2","first-page":"147","volume":"67","author":"Mole C. M.","year":"1992","journal-title":"Lab Invest."},{"key":"e_1_2_1_26_2","first-page":"559","volume":"29","author":"Brandtzaeg P.","year":"1975","journal-title":"Immunology"},{"key":"e_1_2_1_27_2","first-page":"295","volume":"62","author":"Diamandis E. P.","year":"2002","journal-title":"Cancer Res."},{"key":"e_1_2_1_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0009-9120(00)00145-4"},{"key":"e_1_2_1_29_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01960136"},{"key":"e_1_2_1_30_2","doi-asserted-by":"publisher","DOI":"10.1002\/j.1536-4801.1990.tb10029.x"},{"key":"e_1_2_1_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)67380-6"},{"key":"e_1_2_1_32_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.26.18237"},{"key":"e_1_2_1_33_2","doi-asserted-by":"publisher","DOI":"10.3168\/jds.S0022-0302(72)85741-2"},{"key":"e_1_2_1_34_2","doi-asserted-by":"publisher","DOI":"10.1203\/00006450-198206000-00016"},{"key":"e_1_2_1_35_2","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2760(90)90213-H"},{"key":"e_1_2_1_36_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.191.10.1807"},{"key":"e_1_2_1_37_2","doi-asserted-by":"publisher","DOI":"10.1203\/00006450-199810000-00006"},{"key":"e_1_2_1_38_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0008-6215(97)00273-5"},{"key":"e_1_2_1_39_2","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.20277"},{"key":"e_1_2_1_40_2","doi-asserted-by":"publisher","DOI":"10.1002\/1522-2683(200204)23:7\/8<1153::AID-ELPS1153>3.0.CO;2-Y"},{"key":"e_1_2_1_41_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1651-2227.1987.tb10469.x"},{"key":"e_1_2_1_42_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9861(86)90537-0"},{"key":"e_1_2_1_43_2","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1093\/ajcn\/48.2.295","volume":"48","author":"Oizumi J.","year":"1988","journal-title":"Am. J. Clin. Nutr."},{"key":"e_1_2_1_44_2","first-page":"297","volume":"25","author":"Armogida S. A.","year":"2004","journal-title":"Allergy Asthma Proc."},{"key":"e_1_2_1_45_2","doi-asserted-by":"publisher","DOI":"10.1002\/1522-2683(200105)22:9<1810::AID-ELPS1810>3.0.CO;2-M"},{"key":"e_1_2_1_46_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1046-5928(03)00102-5"},{"key":"e_1_2_1_47_2","first-page":"921","volume":"30","author":"Vetvicka V.","year":"1993","journal-title":"Biochem. Mol. Biol. Int."},{"key":"e_1_2_1_48_2","doi-asserted-by":"publisher","DOI":"10.1002\/j.1536-4801.1997.tb00670.x"},{"key":"e_1_2_1_49_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi00482a015"},{"key":"e_1_2_1_50_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0897.1996.tb00007.x"},{"key":"e_1_2_1_51_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-291X(05)81415-9"},{"key":"e_1_2_1_52_2","doi-asserted-by":"publisher","DOI":"10.1271\/bbb.59.713"},{"key":"e_1_2_1_53_2","doi-asserted-by":"publisher","DOI":"10.1159\/000231858"},{"key":"e_1_2_1_54_2","doi-asserted-by":"publisher","DOI":"10.1203\/00006450-198406000-00010"},{"key":"e_1_2_1_55_2","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1542\/peds.70.2.235","volume":"70","author":"Lindberg T.","year":"1982","journal-title":"Pediatrics"},{"key":"e_1_2_1_56_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0022029901005337"},{"key":"e_1_2_1_57_2","first-page":"281","volume":"9","author":"Agrimonti F.","year":"1982","journal-title":"Chronobiologia"},{"key":"e_1_2_1_58_2","first-page":"569","volume":"41","author":"Kalembey I.","year":"1997","journal-title":"Int. J. Dev. Biol."},{"key":"e_1_2_1_59_2","doi-asserted-by":"publisher","DOI":"10.1055\/s-2007-1003514"},{"key":"e_1_2_1_60_2","doi-asserted-by":"publisher","DOI":"10.1016\/0167-4838(95)00173-5"},{"key":"e_1_2_1_61_2","doi-asserted-by":"publisher","DOI":"10.1016\/0305-0491(79)90181-0"},{"key":"e_1_2_1_62_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2249.1999.00782.x"},{"key":"e_1_2_1_63_2","doi-asserted-by":"publisher","DOI":"10.1016\/0020-711X(89)90201-2"},{"key":"e_1_2_1_64_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(85)81220-5"}],"container-title":["PROTEOMICS"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fpmic.200500558","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/pmic.200500558","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T15:51:25Z","timestamp":1761061885000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/pmic.200500558"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,3,22]]},"references-count":63,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2006,4]]}},"alternative-id":["10.1002\/pmic.200500558"],"URL":"https:\/\/doi.org\/10.1002\/pmic.200500558","archive":["Portico"],"relation":{},"ISSN":["1615-9853","1615-9861"],"issn-type":[{"value":"1615-9853","type":"print"},{"value":"1615-9861","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,3,22]]},"assertion":[{"value":"2005-07-25","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2006-03-22","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}