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Proteins from a stripped plasma membrane fraction were solubilized with the neutral and non\u2010denaturing detergent, the <jats:italic>n<\/jats:italic>\u2010dodecyl \u03b2\u2010<jats:sc>D<\/jats:sc>\u2010maltoside. Proteins were then resolved using a recently described ion\u2010exchange chromatography (IEC)\/lithium dodecyl sulfate (LDS)\u2010PAGE procedure. Nearly 90\u2005proteins were identified and clustered, based on their p<jats:italic>I<\/jats:italic>, molecular weight, abundance and\/or hydrophobicity. This method was then applied to profile the plasma membrane response to calcofluor. The LDS\u2010PAGE patterns obtained from whole plasma membrane proteins were similar for the non\u2010treated and calcofluor\u2010treated samples. However, IEC\/LDS\u2010PAGE analysis revealed subtle changes in the expression of several proteins of low abundance, in response to calcofluor. These proteins include Pil1p and Lsp1p, two sphingolipid long\u2010chain base\u2010responsive inhibitors of protein kinases involved in signaling pathways for cell wall integrity and Rho1p, a small GTPase. It was recently hypothesized that Pil1p and Lsp1p could associate with, and regulate, the plasma membrane \u03b2\u20101\u20103\u2010glucan synthase, responsible for the synthesis of another major microfibril for yeast cell wall. Results are discussed with respect to both calcofluor effects on the plasma membrane proteins and the power of the IEC\/LDS\u2010PAGE procedure in the search for new potential therapeutics targets.<\/jats:p>","DOI":"10.1002\/pmic.200500762","type":"journal-article","created":{"date-parts":[[2006,4,18]],"date-time":"2006-04-18T14:12:19Z","timestamp":1145369539000},"page":"3029-3039","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":32,"title":["The plasma membrane proteome of <b><i>Saccharomyces cerevisiae<\/i><\/b> and its response to the antifungal calcofluor"],"prefix":"10.1002","volume":"6","author":[{"given":"Fr\u00e9d\u00e9ric","family":"Delom","sequence":"first","affiliation":[]},{"given":"Wojciech","family":"Szponarski","sequence":"additional","affiliation":[]},{"given":"Nicolas","family":"Sommerer","sequence":"additional","affiliation":[]},{"given":"Jean\u2010Christophe","family":"Boyer","sequence":"additional","affiliation":[]},{"given":"Jean\u2010Michel","family":"Bruneau","sequence":"additional","affiliation":[]},{"given":"Michel","family":"Rossignol","sequence":"additional","affiliation":[]},{"given":"R\u00e9my","family":"Gibrat","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2006,5,11]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-7799(01)01811-X"},{"key":"e_1_2_1_3_2","first-page":"1577","volume":"129","author":"Elorza M. 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