{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,10]],"date-time":"2026-03-10T20:01:44Z","timestamp":1773172904018,"version":"3.50.1"},"reference-count":50,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2008,12,31]],"date-time":"2008-12-31T00:00:00Z","timestamp":1230681600000},"content-version":"vor","delay-in-days":4962,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Protein Science"],"published-print":{"date-parts":[[1995,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Cellobiohydrolase I (CBHI) of <jats:italic>Trichoderma reesei<\/jats:italic> has two functional domains, a catalytic core domain and a cellulose binding domain (CBD). The structure of the CBD reveals two distinct faces, one of which is flat and the other rough. Several other fungal cellulolytic enzymes have similar two\u2010domain structures, in which the CBDs show a conserved primary structure. Here we have evaluated the contributions of conserved amino acids in CBHI CBD to its binding to cellulose. Binding isotherms were determined for a set of six synthetic analogues in which conserved amino acids were substituted. Two\u2010dimensional NMR spectroscopy was used to assess the structural effects of the substitutions by comparing chemical shifts, coupling constants, and NOEs of the backbone protons between the wild\u2010type CBD and the analogues. In general, the structural effects of the substitutions were minor, although in some cases decreased binding could clearly be ascribed to conformational perturbations. We found that at least two tyrosine residues and a glutamine residue on the flat face were essential for tight binding of the CBD to cellulose. A change on the rough face had only a small effect on the binding and it is unlikely that this face interacts with cellulose directly.<\/jats:p>","DOI":"10.1002\/pro.5560040604","type":"journal-article","created":{"date-parts":[[2010,6,29]],"date-time":"2010-06-29T08:47:45Z","timestamp":1277801265000},"page":"1056-1064","source":"Crossref","is-referenced-by-count":188,"title":["Identification of functionally important amino acids in the cellulose\u2010binding domain of <i>Trichoderma reesei<\/i> cellobiohydrolase I"],"prefix":"10.1002","volume":"4","author":[{"given":"Markus","family":"Linder","sequence":"first","affiliation":[]},{"given":"Maija\u2010Liisa","family":"Mattinen","sequence":"additional","affiliation":[]},{"given":"Maarit","family":"Kontteli","sequence":"additional","affiliation":[]},{"given":"Gunnar","family":"Lindeberg","sequence":"additional","affiliation":[]},{"given":"Jerry","family":"St\u00e5hlberg","sequence":"additional","affiliation":[]},{"given":"Torbj\u00f6rn","family":"Drakenberg","sequence":"additional","affiliation":[]},{"given":"Tapani","family":"Reinikainen","sequence":"additional","affiliation":[]},{"given":"G\u00d6Ran","family":"Pettersson","sequence":"additional","affiliation":[]},{"given":"Arto","family":"Annila","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2008,12,31]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-136-12-2569"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2364(85)90018-6"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6976.1994.tb00033.x"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/bit.260300215"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(77)80200-3"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2364(83)90226-3"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2364(90)90109-M"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/18.18.5559"},{"key":"e_1_2_1_10_1","doi-asserted-by":"crossref","first-page":"2779","DOI":"10.1128\/aem.60.8.2779-2785.1994","article-title":"The cel3 gene of Agaricus bisporus codes for a modular cellulase and is transcriptually regulated by the carbon source","volume":"60","author":"Chow CM","year":"1994","journal-title":"Appl Environ Microbiol"},{"key":"e_1_2_1_11_1","first-page":"1","volume-title":"Trichoderma reesei celluloses: Biochemistry, genetics, physiology and application","author":"Claeyssens M","year":"1989"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1992.tb01563.x"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/314235a0"},{"key":"e_1_2_1_14_1","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1128\/mr.55.2.303-315.1991","article-title":"Domains in microbial \u03b2\u20131,4\u2010glycanases: Sequence conservation, function, and enzyme families","volume":"55","author":"Gilkes NR","year":"1991","journal-title":"Microbiol Rev"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)50488-4"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(88)80726-9"},{"key":"e_1_2_1_17_1","first-page":"4","article-title":"Bacterial cellulose","volume":"3","author":"Herstin S.","year":"1963","journal-title":"Methods Carbohydr Chem"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(89)80168-1"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja00901a059"},{"key":"e_1_2_1_20_1","first-page":"25","volume-title":"Protein function","author":"Klotz IM","year":"1989"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00351a003"},{"key":"e_1_2_1_22_1","first-page":"57","article-title":"Cloning, sequencing, and heterologous expression of a cellulase encoding cDNA (cbh1) from","volume":"124","author":"Koch A","year":"1993","journal-title":"Penicillium janitellum. Gene"},{"key":"e_1_2_1_23_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00444a016"},{"key":"e_1_2_1_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2364(92)90035-6"},{"key":"e_1_2_1_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-3011.1991.tb01436.x"},{"key":"e_1_2_1_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2836(92)90560-7"},{"key":"e_1_2_1_27_1","doi-asserted-by":"publisher","DOI":"10.1080\/00268978000102601"},{"key":"e_1_2_1_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(83)91093-8"},{"key":"e_1_2_1_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2364(92)90098-R"},{"key":"e_1_2_1_30_1","doi-asserted-by":"publisher","DOI":"10.1002\/bit.260420402"},{"key":"e_1_2_1_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2836(84)90035-4"},{"key":"e_1_2_1_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(86)90023-5"},{"key":"e_1_2_1_33_1","doi-asserted-by":"publisher","DOI":"10.1042\/bst0210442"},{"key":"e_1_2_1_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(92)90270-Y"},{"key":"e_1_2_1_35_1","unstructured":"RasmussenG MikkelsenJ SchuleinM HagenF HjortC HastrupS.1991. A cellulase preparation comprising an endoglucanase enzyme. World Pat WO 91\/17243. pp 50\u201351."},{"key":"e_1_2_1_36_1","doi-asserted-by":"publisher","DOI":"10.1002\/prot.340140408"},{"key":"e_1_2_1_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-3233(08)60520-3"},{"key":"e_1_2_1_38_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1994.tb00417.x"},{"key":"e_1_2_1_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(88)90541-0"},{"key":"e_1_2_1_40_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt1083-691"},{"key":"e_1_2_1_41_1","first-page":"411","article-title":"The identification, molecular cloning and characterization of a gene from Phanerochaete chrysosporium that shows strong homology to the exo\u2010cellobiohydrolase I gene from","volume":"74","author":"Sims P","year":"1988","journal-title":"Trichoderma reesei. Gene"},{"key":"e_1_2_1_42_1","doi-asserted-by":"publisher","DOI":"10.1006\/jmra.1993.1192"},{"key":"e_1_2_1_43_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt0391-286"},{"key":"e_1_2_1_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(87)90472-0"},{"key":"e_1_2_1_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/0168-1656(92)90120-X"},{"key":"e_1_2_1_46_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1988.tb13736.x"},{"key":"e_1_2_1_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(86)80816-X"},{"key":"e_1_2_1_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/0959-440X(91)90172-P"},{"key":"e_1_2_1_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2364(83)90284-6"},{"key":"e_1_2_1_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2836(86)90413-4"},{"key":"e_1_2_1_51_1","doi-asserted-by":"publisher","DOI":"10.1042\/bst0200046"}],"container-title":["Protein Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fpro.5560040604","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/pro.5560040604","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,26]],"date-time":"2023-10-26T21:04:51Z","timestamp":1698354291000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/pro.5560040604"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1995,6]]},"references-count":50,"journal-issue":{"issue":"6","published-print":{"date-parts":[[1995,6]]}},"alternative-id":["10.1002\/pro.5560040604"],"URL":"https:\/\/doi.org\/10.1002\/pro.5560040604","archive":["Portico"],"relation":{},"ISSN":["0961-8368","1469-896X"],"issn-type":[{"value":"0961-8368","type":"print"},{"value":"1469-896X","type":"electronic"}],"subject":[],"published":{"date-parts":[[1995,6]]}}}