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In addition to d\u2010alanine\u2010d\u2010alanine ligase, glutathione synthetase, biotin carboxylase, and carbamoyl phosphate synthetase, enzymes with known three\u2010dimensional structures, the ATP\u2010grasp domain is predicted in the ribosomal protein S6 modification enzyme (RimK), urea amidolyase, tubulin\u2010tyrosine ligase, and three enzymes of purine biosynthesis. All these enzymes possess ATP\u2010dependent carboxylate\u2010amine ligase activity, and their catalytic mechanisms are likely to include acylphosphate intermediates. The ATP\u2010grasp superfamily also includes succinate\u2010CoA ligase (both ADP\u2010forming and GDP\u2010forming variants), malate\u2010CoA ligase, and ATP\u2010citrate lyase, enzymes with a carboxylate\u2010thiol ligase activity, and several uncharacterized proteins. These findings significantly extend the variety of the substrates of ATP\u2010grasp enzymes and the range of biochemical pathways in which they are involved, and demonstrate the complementarity between structural comparison and powerful methods for sequence analysis.<\/jats:p>","DOI":"10.1002\/pro.5560061218","type":"journal-article","created":{"date-parts":[[2009,2,10]],"date-time":"2009-02-10T05:03:22Z","timestamp":1234242202000},"page":"2639-2643","source":"Crossref","is-referenced-by-count":242,"title":["A diverse superfamily of enzymes with ATP\u2010dependent carboxylate\u2014amine\/thiol ligase activity"],"prefix":"10.1002","volume":"6","author":[{"given":"Michael Y.","family":"Galperin","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eugene V.","family":"Koonin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2008,12,31]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(96)66029-7"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(05)80360-2"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/25.17.3389"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0167-4838(93)90125-B"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/nsb0296-128"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/16.12.3416"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.273.5278.1058"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02338803"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.7939684"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.4.1172"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.7542800"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.270.5235.397"},{"key":"e_1_2_1_14_1","first-page":"210","article-title":"Purification and characterization of glutathione synthetase from Escherichia coli B","volume":"5","author":"Gushima H","year":"1983","journal-title":"J Appl Biochem"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.7.2652"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/nsb0196-16"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(96)66041-8"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1093\/dnares\/3.3.109"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02464894"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9861(77)90186-2"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1997.4821861.x"},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.1093\/protein\/9.12.1083"},{"key":"e_1_2_1_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/9780470123089.ch7"},{"key":"e_1_2_1_24_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00174a038"},{"key":"e_1_2_1_25_1","doi-asserted-by":"crossref","first-page":"6247","DOI":"10.1016\/S0021-9258(19)44789-3","article-title":"Nucleotide specificity of Escherichia coli succinic thiokinase. 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