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Twenty to 30% of all ORFs are predicted to encode membrane proteins, with the larger genomes containing a higher fraction than the smaller ones. Although there is a general tendency that proteins with a smaller number of transmembrane segments are more prevalent than those with many, uni\u2010cellular organisms appear to prefer proteins with 6 and 12 transmembrane segments, whereas <jats:italic>Caenorhabditis elegans<\/jats:italic>and<jats:italic>Homo sapiens<\/jats:italic>have a slight preference for proteins with seven transmembrane segments. In all organisms, there is a tendency that membrane proteins either have manytransmembrane segments with short connecting loops or few transmembrane segments with large extra\u2010membraneous domains. Membrane proteins from all organisms studied, except possibly the archaeon <jats:italic>Methanococcus jannaschii<\/jats:italic>, follow the so\u2010called \u201cpositive\u2010inside\u201d rule; i.e., they tend to have a higher frequency of positively charged residues in cytoplasmic than in extra\u2010cytoplasmic segments.<\/jats:p>","DOI":"10.1002\/pro.5560070420","type":"journal-article","created":{"date-parts":[[2010,7,12]],"date-time":"2010-07-12T05:50:28Z","timestamp":1278913828000},"page":"1029-1038","source":"Crossref","is-referenced-by-count":1290,"title":["Genome\u2010wide analysis of integral membrane proteins from eubacterial, archaean, and eukaryotic organisms"],"prefix":"10.1002","volume":"7","author":[{"given":"Erik","family":"Wallin","sequence":"first","affiliation":[]},{"given":"Gunnar 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