{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,21]],"date-time":"2026-03-21T20:58:19Z","timestamp":1774126699850,"version":"3.50.1"},"reference-count":19,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2008,12,31]],"date-time":"2008-12-31T00:00:00Z","timestamp":1230681600000},"content-version":"vor","delay-in-days":3927,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"crossref","award":["Grant La699\/4- 1"],"award-info":[{"award-number":["Grant La699\/4- 1"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Protein Science"],"published-print":{"date-parts":[[1998,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The glycophorin A transmembrane segment homodimerizes to a right\u2010handed pair of \u03b1\u2010helices. Here, we identified the amino acid motif mediating this interaction within a natural membrane environment. Critical residues were grafted onto two different hydrophobic host sequences in a stepwise manner and self\u2010assembly of the hybrid sequences was determined with the ToxR transcription activator system. Our results show that the motif LIxxGxxxGxxxT elicits a level of self\u2010association equivalent to that of the original glycophorin A transmembrane segment. This motif is very similar to the one previously established in detergent solution. Interestingly, the central GxxxG motif by itself already induced strong self\u2010assembly of host sequences and the three\u2010residue spacing between both glycines proved to be optimal for the interaction. The GxxxG element thus appears to be the most crucial part of the interaction motif.<\/jats:p>","DOI":"10.1002\/pro.5560070423","type":"journal-article","created":{"date-parts":[[2010,7,12]],"date-time":"2010-07-12T09:28:50Z","timestamp":1278926930000},"page":"1052-1056","source":"Crossref","is-referenced-by-count":205,"title":["The dimerization motif of the glycophorin A transmembrane segment in membranes: Importance of glycine residues"],"prefix":"10.1002","volume":"7","author":[{"given":"Bettina","family":"Brosig","sequence":"first","affiliation":[]},{"given":"Dieter","family":"Langosch","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2008,12,31]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0134(199611)26:3<257::AID-PROT2>3.0.CO;2-B"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.44.26404"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1988.tb13823.x"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.23.14115"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1329208"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0959-440X(97)80111-X"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1992.tb01489.x"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.27.16384"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1515\/bchm3.1994.375.1.61"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1995.tb00061.x"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/0076-6879(87)54085-X"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.1996.0595"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0033583500004522"},{"key":"e_1_2_1_15_1","doi-asserted-by":"crossref","first-page":"7683","DOI":"10.1016\/S0021-9258(18)42569-0","article-title":"Glycophorin A dimerization is driven by specific interactions between transmembrane alpha\u2010helizes","volume":"267","author":"Lemmon MA","year":"1992","journal-title":"J Biol Chem"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00166a002"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/nsb0394-157"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.276.5309.131"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/pro.5560050712"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.1995.0469"}],"container-title":["Protein Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fpro.5560070423","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fpro.5560070423","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/pro.5560070423","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,28]],"date-time":"2023-10-28T17:41:31Z","timestamp":1698514891000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/pro.5560070423"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1998,4]]},"references-count":19,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1998,4]]}},"alternative-id":["10.1002\/pro.5560070423"],"URL":"https:\/\/doi.org\/10.1002\/pro.5560070423","archive":["Portico"],"relation":{},"ISSN":["0961-8368","1469-896X"],"issn-type":[{"value":"0961-8368","type":"print"},{"value":"1469-896X","type":"electronic"}],"subject":[],"published":{"date-parts":[[1998,4]]}}}