{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,11]],"date-time":"2026-03-11T17:59:06Z","timestamp":1773251946339,"version":"3.50.1"},"reference-count":64,"publisher":"Wiley","issue":"13","license":[{"start":{"date-parts":[[2014,7,22]],"date-time":"2014-07-22T00:00:00Z","timestamp":1405987200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/3.0\/"}],"funder":[{"name":"Science Foundation Ireland's funding of Molecular Therapeutics for Cancer, Ireland","award":["08\/SRC\/B1410"],"award-info":[{"award-number":["08\/SRC\/B1410"]}]},{"name":"EU Co-operation in Science and Technology, ME-HaD","award":["BM1202"],"award-info":[{"award-number":["BM1202"]}]},{"name":"Higher Education Authority's Programme for Research in Third Level Institutions Cycle 5"},{"name":"Trinity Biomedical Sciences Institute"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Prostate"],"published-print":{"date-parts":[[2014,9]]},"abstract":"<jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>Docetaxel\u2010resistance limits successful treatment of castration resistant prostate cancer. We previously demonstrated that extracellular vesicles (exosomes) may play a role in regulating docetaxel resistance. Here, we investigated intracellular and extracellular (exosomal) miRNAs related to docetaxel resistance.<\/jats:p><\/jats:sec><jats:sec><jats:title>METHODS<\/jats:title><jats:p>Following global miRNA profiling of cell line models of docetaxel\u2010resistance and their corresponding exosomes, we investigated the clinical relevance of four selected miRNAs (miR\u2010598, miR\u201034a, miR\u2010146a, miR\u2010148a) in four publically available clinical cohorts representing both primary and advanced disease in tissue and urine specimens. One of these miRNAs, miR\u201034a was selected for functional evaluation by miRNA inhibition and over\u2010expression in vitro. We further assessed the panel of miRNAs for their combined clinical relevance as a biomarker signature by examining their common predicted targets.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>A strong correlation was found between the detection of miRNAs in exosomes and their corresponding cells of origin. Of the miRNAs chosen for further validation and clinical assessment, decreased miR\u201034a levels showed substantial clinical relevance and so was chosen for further analysis. Manipulating miR\u201034a in prostate cancer cells confirms that this miRNA regulates BCL\u20102 and may, in part, regulate response to docetaxel. When combined, these miRNAs are predicted to regulate a range of common mRNA targets, two of which (e.g., SNCA, SCL7A5) demonstrate a strong relationship with prostate cancer progression and poor prognosis.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSIONS<\/jats:title><jats:p>This study supports the extracellular environment as an important source of minimally invasive predictive biomarkers representing their cellular origin. Using miR\u201034a as example, we showed that biomarkers identified in this manner may also hold functional relevance. <jats:italic>Prostate 74: 1320\u20131334, 2014<\/jats:italic>. \u00a9 2014 The Authors. <jats:italic>The Prostate<\/jats:italic>, published by Wiley Periodicals, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/pros.22848","type":"journal-article","created":{"date-parts":[[2014,7,22]],"date-time":"2014-07-22T19:03:33Z","timestamp":1406055813000},"page":"1320-1334","source":"Crossref","is-referenced-by-count":194,"title":["miR\u201034a is an intracellular and exosomal predictive biomarker for response to docetaxel with clinical relevance to prostate cancer progression"],"prefix":"10.1002","volume":"74","author":[{"given":"Claire","family":"Corcoran","sequence":"first","affiliation":[{"name":"School of Pharmacy and Pharmaceutical Sciences &amp; Trinity Biomedical Sciences Institute Trinity College Dublin Dublin Ireland"}]},{"given":"Sweta","family":"Rani","sequence":"additional","affiliation":[{"name":"School of Pharmacy and Pharmaceutical Sciences &amp; Trinity Biomedical Sciences Institute Trinity College Dublin Dublin Ireland"}]},{"given":"Lorraine","family":"O'Driscoll","sequence":"additional","affiliation":[{"name":"School of Pharmacy and Pharmaceutical Sciences &amp; Trinity Biomedical Sciences Institute Trinity College Dublin Dublin 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