{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,6,26]],"date-time":"2025-06-26T18:04:43Z","timestamp":1750961083299},"reference-count":18,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2006,7,20]],"date-time":"2006-07-20T00:00:00Z","timestamp":1153353600000},"content-version":"vor","delay-in-days":5679,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Prostate"],"published-print":{"date-parts":[[1991,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The DU145 human prostate cancer cell line possesses epidermal growth factor (EGF) receptors and synthesizes both EGF and the related polypeptide transforming growth factor \u03b1 (TGF\u2010\u03b1). A monoclonal antibody to the EGF receptor was used to determine whether these characteristics were indicative of a functional autocrine regulatory system. This antibody competed effectively with [<jats:sup>125<\/jats:sup>I]EGF for binding to DU145 cell binding sites over a 1 \u00d7 10<jats:sup>\u221211<\/jats:sup> to 1 \u00d7 10<jats:sup>\u22127<\/jats:sup> M concentration range, and did so with a capability similar to that of the two natural ligands. It inhibited growth of these cells in both 3% fetal bovine serum\u2010supplemented and serum\u2010free medium; in experiments with incubation times of 3\u20135 days there was a 45\u201350% reduction in cell number. Growth suppression by the EGF receptor blockade of cells plated at a density of 1.5 \u00d7 10<jats:sup>4<\/jats:sup> cells\/ml\/well was reversed competitively by the addition of EGF to the medium; 0.3 nM completely eliminated the inhibitory effect of a 1 \u00d7 10<jats:sup>\u22129<\/jats:sup> M antibody concentration. It is concluded that DU145 cell growth is regulated by an EGF\u2010mediated autocrine loop.<\/jats:p>","DOI":"10.1002\/pros.2990190210","type":"journal-article","created":{"date-parts":[[2007,2,21]],"date-time":"2007-02-21T02:08:46Z","timestamp":1172023726000},"page":"173-180","source":"Crossref","is-referenced-by-count":49,"title":["Autocrine regulation of DU145 human prostate cancer cell growth by epidermal growth factor\u2010related polypeptides"],"prefix":"10.1002","volume":"19","author":[{"given":"Jeanne M.","family":"Connolly","sequence":"first","affiliation":[]},{"given":"David P.","family":"Rose","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2006,7,20]]},"reference":[{"key":"e_1_2_1_2_2","first-page":"4069","article-title":"Presence of transforming growth factors in human breast cancer cells","volume":"44","author":"Salomon DS","year":"1984","journal-title":"Cancer Res"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.240350102"},{"key":"e_1_2_1_4_2","first-page":"4590","article-title":"Transforming growth factor \u03b1 and \u03b2 expression in human colon cancer lines: implications for an autocrine model","volume":"47","author":"Coffey RJ","year":"1987","journal-title":"Cancer Res"},{"key":"e_1_2_1_5_2","first-page":"707","article-title":"Synthesis of messenger RNAs for transforming growth factors \u03b1 and \u03b2 and the epidermal growth factor receptor by human tumors","volume":"47","author":"Derynck R","year":"1987","journal-title":"Cancer Res"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/bjc.1989.159"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/pros.2990150211"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/pros.2990150102"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1002\/pros.2990160304"},{"key":"e_1_2_1_10_2","first-page":"57","volume-title":"Endocrinology of Cancer","author":"Hodges CV","year":"1979"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910210305"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1210\/mend-3-11-1830"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3835(87)90108-X"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1002\/pros.2990120108"},{"key":"e_1_2_1_15_2","first-page":"51","article-title":"Epidermal growth factor\u2010mediated regulation of DU145 prostate cancer cell growth","volume":"32","author":"Tillotson JK","year":"1991","journal-title":"Proc Am Assoc Cancer Res"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/80.13.1053"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1210\/mend-1-3-216"},{"key":"e_1_2_1_18_2","first-page":"241","article-title":"The role of transforming growth factors \u03b1 and \u03b2 in human prostate cancer cell growth","volume":"29","author":"Wilding G","year":"1988","journal-title":"Proc Am Assoc Cancer Res"},{"key":"e_1_2_1_19_2","doi-asserted-by":"publisher","DOI":"10.1210\/mend-2-6-543"}],"container-title":["The Prostate"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fpros.2990190210","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/pros.2990190210","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,23]],"date-time":"2023-10-23T02:54:28Z","timestamp":1698029668000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/pros.2990190210"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1991,1]]},"references-count":18,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1991,1]]}},"alternative-id":["10.1002\/pros.2990190210"],"URL":"https:\/\/doi.org\/10.1002\/pros.2990190210","archive":["Portico"],"relation":{},"ISSN":["0270-4137","1097-0045"],"issn-type":[{"value":"0270-4137","type":"print"},{"value":"1097-0045","type":"electronic"}],"subject":[],"published":{"date-parts":[[1991,1]]}}}