{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,8]],"date-time":"2026-02-08T17:10:03Z","timestamp":1770570603099,"version":"3.49.0"},"reference-count":67,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2004,2,3]],"date-time":"2004-02-03T00:00:00Z","timestamp":1075766400000},"content-version":"vor","delay-in-days":4295,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Proteins"],"published-print":{"date-parts":[[1992,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A recently developed algorithm, called Convex Constraint Analysis (CCA), was successfully applied to determine the circular dichroism (CD) spectra of the <jats:italic>pure<\/jats:italic> \u03b2\u2010<jats:italic>pleated sheet<\/jats:italic> in globular proteins. On the basis of X\u2010ray diffraction determined secondary structures, the original data set used (Perczel, A., Hollosi, M., Tusnady, G. Fasman, G.D. Convex constraint analysis: A natural deconvolution of circular dichroism curves of proteins, Prot. Eng., <jats:italic>4<\/jats:italic>:\u2010669\u2013679, 1991), was improved by the addition of proteins with high \u03b2\u2010pleated sheet content. The analysis yielded CD curves of the pure components of the main secondary structural elements (\u03b1\u2010helix, antiparallel \u03b2\u2010pleated sheet, \u03b2\u2010turns, and unordered conformation), as well as a curve attributed to the \u201caromatic contribution\u201d in the wavelength range of 195\u2013240 nm. Upon deconvolution the curves obtained were assigned to various secondary structures. The calculated weights (percentages determining the contributions of each pure component curve in the measured CD spectra of a given protein) were correlated with the X\u2010ray diffraction determined percentages in an assignment procedure and were evaluated. The Pearson product correlation coefficients (<jats:italic>R<\/jats:italic>) are significant for all five components. The new pure component curves, which were obtained through deconvolution of the protein CD spectra alone, are promising candidates for determining the percentages of the secondary structural components in globular proteins without the necessity of adopting an X\u2010ray database. The CD spectrum of the CheY protein was interesting because it has the characteristic shape associated with the \u03b1\u2010helical structure, but upon analysis yielded a considerable amount of \u03b2\u2010sheet in agreement with the X\u2010ray structure. \u00a9 1992 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/prot.340130106","type":"journal-article","created":{"date-parts":[[2005,5,29]],"date-time":"2005-05-29T01:17:30Z","timestamp":1117329450000},"page":"57-69","source":"Crossref","is-referenced-by-count":94,"title":["Deconvolution of the circular dichroism spectra of proteins: The circular dichroism spectra of the antiparallel \u03b2\u2010sheet in proteins"],"prefix":"10.1002","volume":"13","author":[{"given":"A.","family":"Perczel","sequence":"first","affiliation":[]},{"given":"K.","family":"Park","sequence":"additional","affiliation":[]},{"given":"Gerald D.","family":"Fasman","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2004,2,3]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.37.4.205"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1107\/S0108767387099239"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.37.11.729"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2836(73)90022-3"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(05)80193-7"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.85.10.3338"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/0079-6107(83)90005-6"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-304207-1.50008-4"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1002\/prot.340070302"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi00504a006"},{"key":"e_1_2_1_12_2","first-page":"189","article-title":"Prediction of secondary structure of globular proteins using CD spectra","volume":"24","author":"Shubin U. 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