{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T00:24:59Z","timestamp":1777422299530,"version":"3.51.4"},"reference-count":51,"publisher":"Wiley","issue":"16","license":[{"start":{"date-parts":[[2011,5,31]],"date-time":"2011-05-31T00:00:00Z","timestamp":1306800000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Small"],"published-print":{"date-parts":[[2011,8,22]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Single\u2010wall carbon nanotubes (SWCNTs) possess many unique, inherent properties that make them attractive materials for application in medical and biological technologies. Development of concentrated SWCNT dispersions of isolated nanotubes that retain SWCNTs' inherent properties with minimal negative cellular effects is essential to fully realize the potential of SWCNTs in biotechnology. It is shown that bovine serum albumin (BSA), a common and well\u2010characterized model blood serum protein, can individually disperse SWCNTs at concentrations of up to 0.3 mg mL<jats:sup>\u22121<\/jats:sup> while retaining SWCNTs' optical properties. Uptake into human mesenchymal stem cells (hMSC) and HeLa cells is quantified, revealing strikingly high concentrations of 86 \u00b1 33 \u00d7 10<jats:sup>6<\/jats:sup> and 21 \u00b1 13 \u00d7 10<jats:sup>6<\/jats:sup> SWCNTs per cell, respectively, without any apparent acute deleterious cellular effects. Through high\u2010resolution confocal Raman spectroscopy and imaging, it is established that SWCNT\u2013BSAs are preferentially localized intracellularly, especially in the cytoplasm of both hMSCs and HeLa cells. The uptake and localization results demonstrate the efficacy of BSA as a biocompatible dispersant and a mediator of bioactivity. BSA is widely available and inexpensive, which make these concentrated, highly\u2010dispersed, noncovalently modified SWCNT\u2013BSAs suitable for the development of SWCNT\u2010based biotechnologies.<\/jats:p>","DOI":"10.1002\/smll.201100437","type":"journal-article","created":{"date-parts":[[2011,5,31]],"date-time":"2011-05-31T09:14:46Z","timestamp":1306833286000},"page":"2348-2355","source":"Crossref","is-referenced-by-count":101,"title":["Quantification of Uptake and Localization of Bovine Serum Albumin\u2010Stabilized Single\u2010Wall Carbon Nanotubes in Different Human Cell Types"],"prefix":"10.1002","volume":"7","author":[{"given":"Brian D.","family":"Holt","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kris Noel","family":"Dahl","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohammad F.","family":"Islam","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2011,5,31]]},"reference":[{"key":"e_1_2_7_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2006.07.044"},{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbpa.2005.10.005"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0502680102"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0904148106"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1063\/1.2103398"},{"key":"e_1_2_7_6_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.273.5274.483"},{"key":"e_1_2_7_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/381678a0"},{"key":"e_1_2_7_8_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1101398"},{"key":"e_1_2_7_9_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1072631"},{"key":"e_1_2_7_10_2","doi-asserted-by":"publisher","DOI":"10.1103\/PhysRevLett.93.037404"},{"key":"e_1_2_7_11_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja0466311"},{"key":"e_1_2_7_12_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0609265103"},{"key":"e_1_2_7_13_2","doi-asserted-by":"publisher","DOI":"10.1021\/nl048326j"},{"key":"e_1_2_7_14_2","doi-asserted-by":"publisher","DOI":"10.1021\/nn9011225"},{"key":"e_1_2_7_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2009.07.025"},{"key":"e_1_2_7_16_2","doi-asserted-by":"publisher","DOI":"10.1021\/nl010065f"},{"key":"e_1_2_7_17_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10856-007-3296-y"},{"key":"e_1_2_7_18_2","doi-asserted-by":"publisher","DOI":"10.1021\/nn901479w"},{"key":"e_1_2_7_19_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmat1427"},{"key":"e_1_2_7_20_2","doi-asserted-by":"publisher","DOI":"10.1021\/nn101151x"},{"key":"e_1_2_7_21_2","doi-asserted-by":"publisher","DOI":"10.1021\/nl025924u"},{"key":"e_1_2_7_22_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11095-006-9104-4"},{"key":"e_1_2_7_23_2","doi-asserted-by":"publisher","DOI":"10.1021\/la0528450"},{"key":"e_1_2_7_24_2","doi-asserted-by":"publisher","DOI":"10.1021\/la047994h"},{"key":"e_1_2_7_25_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmat877"},{"key":"e_1_2_7_26_2","doi-asserted-by":"publisher","DOI":"10.1007\/s12274-008-8022-7"},{"key":"e_1_2_7_27_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja807224x"},{"key":"e_1_2_7_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-3495(75)85797-3"},{"key":"e_1_2_7_29_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1973.tb02943.x"},{"key":"e_1_2_7_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-291X(05)80083-X"},{"key":"e_1_2_7_31_2","doi-asserted-by":"publisher","DOI":"10.1021\/j100232a003"},{"key":"e_1_2_7_32_2","doi-asserted-by":"publisher","DOI":"10.1093\/humupd\/3.2.125"},{"key":"e_1_2_7_33_2","doi-asserted-by":"publisher","DOI":"10.1021\/la0528201"},{"key":"e_1_2_7_34_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cplett.2006.08.044"},{"key":"e_1_2_7_35_2","doi-asserted-by":"publisher","DOI":"10.1021\/nn102112b"},{"key":"e_1_2_7_36_2","doi-asserted-by":"publisher","DOI":"10.1021\/jp047521j"},{"key":"e_1_2_7_37_2","doi-asserted-by":"publisher","DOI":"10.1021\/nl050366f"},{"key":"e_1_2_7_38_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1078727"},{"key":"e_1_2_7_39_2","doi-asserted-by":"publisher","DOI":"10.1103\/PhysRevLett.99.137402"},{"key":"e_1_2_7_40_2","doi-asserted-by":"publisher","DOI":"10.1103\/PhysRevLett.95.247401"},{"key":"e_1_2_7_41_2","doi-asserted-by":"publisher","DOI":"10.1103\/PhysRevLett.94.027402"},{"key":"e_1_2_7_42_2","doi-asserted-by":"publisher","DOI":"10.1002\/adma.201001756"},{"key":"e_1_2_7_43_2","first-page":"1017","volume":"91","author":"Lu L.\u2010L.","year":"2006","journal-title":"Haematologica"},{"key":"e_1_2_7_44_2","doi-asserted-by":"publisher","DOI":"10.1002\/adma.200500477"},{"key":"e_1_2_7_45_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.nano.2009.08.001"},{"key":"e_1_2_7_46_2","doi-asserted-by":"publisher","DOI":"10.1021\/nl062306v"},{"key":"e_1_2_7_47_2","doi-asserted-by":"publisher","DOI":"10.1103\/PhysRevB.71.201401"},{"key":"e_1_2_7_48_2","doi-asserted-by":"publisher","DOI":"10.1002\/adma.200602667"},{"key":"e_1_2_7_49_2","doi-asserted-by":"publisher","DOI":"10.1038\/nnano.2006.52"},{"key":"e_1_2_7_50_2","doi-asserted-by":"publisher","DOI":"10.1002\/adma.200702353"},{"key":"e_1_2_7_51_2","doi-asserted-by":"publisher","DOI":"10.1002\/adma.200900498"}],"container-title":["Small"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fsmll.201100437","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/smll.201100437","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,8]],"date-time":"2023-10-08T11:38:25Z","timestamp":1696765105000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/smll.201100437"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,5,31]]},"references-count":51,"journal-issue":{"issue":"16","published-print":{"date-parts":[[2011,8,22]]}},"alternative-id":["10.1002\/smll.201100437"],"URL":"https:\/\/doi.org\/10.1002\/smll.201100437","archive":["Portico"],"relation":{},"ISSN":["1613-6810","1613-6829"],"issn-type":[{"value":"1613-6810","type":"print"},{"value":"1613-6829","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,5,31]]}}}