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Incubation of rat brain coronal sections with 10 nM [<jats:sup>3<\/jats:sup>H]AF\u2010DX 116 showed highest binding site densities in discrete areas such as the superior colliculus and certain thalamic and brainstem nuclei, similar to the distribution reported for [<jats:sup>2<\/jats:sup>H]acetylcholine\/M<jats:sub>2<\/jats:sub> sites. [<jats:sup>3<\/jats:sup>H]AF\u2010DX 116 site densities were markedly lower in forebrain areas such as cortex, striatum, and hippocampus, in contrast to the distribution seen for [<jats:sup>3<\/jats:sup>H]pirenzepine\u2010M<jats:sub>1<\/jats:sub> binding sites, which were concentrated in these forebrain areas; however, differential patterns of labeling were observed for the two muscarinic\u2010M<jats:sub>2<\/jats:sub> probes, [<jats:sup>3<\/jats:sup>H]AF\u2010DX 116 and [<jats:sup>3<\/jats:sup>H]acetylcholine, in the hippocampal formation. Although [<jats:sup>3<\/jats:sup>H]AF\u2010DX 116 binding was broadly distributed in multiple subfields of the hippocampus, [<jats:sup>3<\/jats:sup>H]acetylcholine binding was discretely distributed in a manner resembling that of acetylcholinesterase staining. This suggests the existence of muscarinic\u2010M<jats:sub>2<\/jats:sub> subtypes in the CNS, especially in the hippocampal formation.<\/jats:p>","DOI":"10.1002\/syn.890040205","type":"journal-article","created":{"date-parts":[[2005,1,1]],"date-time":"2005-01-01T13:17:34Z","timestamp":1104585454000},"page":"115-125","source":"Crossref","is-referenced-by-count":40,"title":["Quantitative autoradiographic distribution of [<sup>3<\/sup>H]AF\u2010DX 116 muscarinic\u2010M<sub>2<\/sub> receptor binding sites in rat brain"],"prefix":"10.1002","volume":"4","author":[{"given":"William","family":"Regenold","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dalia M.","family":"Araujo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Remi","family":"Quirion","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,10,12]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00633191"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.1988.tb02497.x"},{"key":"e_1_2_1_4_1","doi-asserted-by":"crossref","unstructured":"Araujo D. 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