{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,28]],"date-time":"2026-01-28T06:53:01Z","timestamp":1769583181709,"version":"3.49.0"},"reference-count":19,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2004,1,29]],"date-time":"2004-01-29T00:00:00Z","timestamp":1075334400000},"content-version":"vor","delay-in-days":3864,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Yeast"],"published-print":{"date-parts":[[1993,7]]},"abstract":"Abstract<\/jats:title>A rapid and convenient method of protein purification involves creating a fusion protein with glutathione S\u2010transferase (GST) (Smith and Johnson, Gene<\/jats:italic> 67<\/jats:bold>, 31\u201340, 1988). In this report, we describe two vectors for the conditional expression of GST fusions in Saccharomyces cerevisiae<\/jats:italic>. The parent plasmid is based on a high\u2010copy, galactose\u2010inducible shuttle vector previously described (Baldari et al., EMBO J.<\/jats:italic> 6<\/jats:bold>, 229\u2013243, 1987). We have demonstrated the use of this system by creating fusions between GST and the yeast RAS2<\/jats:italic> gene. GST\u2010Ras2 fusion proteins undergo the post\u2010translational modifications required for Ras2p to become membrane localized. These vectors provide a useful system for the expression an dpurification of eukaryotic proteins requiring post\u2010translational modification.<\/jats:p>","DOI":"10.1002\/yea.320090705","type":"journal-article","created":{"date-parts":[[2005,5,28]],"date-time":"2005-05-28T23:44:20Z","timestamp":1117323860000},"page":"715-722","source":"Crossref","is-referenced-by-count":250,"title":["Vectors for the inducible overexpression of glutathione S\u2010transferase fusion proteins in yeast"],"prefix":"10.1002","volume":"9","author":[{"given":"David A.","family":"Mitchell","sequence":"first","affiliation":[]},{"given":"Tricia K.","family":"Marshall","sequence":"additional","affiliation":[]},{"given":"Robert J.","family":"Deschenes","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2004,1,29]]},"reference":[{"key":"e_1_2_1_2_1","volume-title":"Current Protocols in Molecular Biology","author":"Ausubel F. M.","year":"1991"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1987.tb04743.x"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(76)90527-3"},{"key":"e_1_2_1_5_1","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1093\/genetics\/93.1.51","article-title":"Selection of lys2 mutants of the yeast Saccharomyces cerevisiae by the utilization of alpha aminoadipate","volume":"93","author":"Chattoo B. B.","year":"1979","journal-title":"Genetics"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.bi.61.070192.002035"},{"key":"e_1_2_1_7_1","first-page":"331","article-title":"Isoprenoid modification and plasma membrane association: Critical factors for ras oncogenicity","volume":"3","author":"Der C. J.","year":"1991","journal-title":"Cancer Cells"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.7.7.2344"},{"key":"e_1_2_1_9_1","doi-asserted-by":"crossref","unstructured":"Frangioni J. V.andNeel B. G.(1993). Solubilization and purification of enzymatically active glutathione S\u2010transferase (pGEX) fusion proteins.Anal. Biochem.in press.","DOI":"10.1006\/abio.1993.1170"},{"key":"e_1_2_1_10_1","doi-asserted-by":"crossref","first-page":"12964","DOI":"10.1016\/S0021-9258(18)98789-2","article-title":"Cloning and expression of a yeast protein tyrosine phosphatase","volume":"266","author":"Guan K.","year":"1991","journal-title":"J. Biol. Chem."},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(91)90534-Z"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.78.4.2199"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(92)90108-J"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.153.1.163-168.1983"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(85)90304-6"},{"key":"e_1_2_1_16_1","doi-asserted-by":"crossref","first-page":"24363","DOI":"10.1016\/S0021-9258(18)35774-0","article-title":"Ras (CXXX) and Rab (CC\/CXC) prenylation signal sequences are unique and functionally distinct","volume":"267","author":"Khosavi\u2010Far R.","year":"1992","journal-title":"J. Biol. Chem."},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/227680a0"},{"key":"e_1_2_1_18_1","volume-title":"Laboratory Course Manual: Methods in Yeast Genetics","author":"Sherman F.","year":"1986"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(88)90005-4"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(85)90305-8"}],"container-title":["Yeast"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fyea.320090705","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/yea.320090705","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,25]],"date-time":"2023-10-25T02:29:42Z","timestamp":1698200982000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/yea.320090705"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1993,7]]},"references-count":19,"journal-issue":{"issue":"7","published-print":{"date-parts":[[1993,7]]}},"alternative-id":["10.1002\/yea.320090705"],"URL":"https:\/\/doi.org\/10.1002\/yea.320090705","archive":["Portico"],"relation":{},"ISSN":["0749-503X","1097-0061"],"issn-type":[{"value":"0749-503X","type":"print"},{"value":"1097-0061","type":"electronic"}],"subject":[],"published":{"date-parts":[[1993,7]]}}}