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One of the major advantages of these plasmids is that they allow polymerase chain reaction\u2010amplified open reading frames to be automatically fused in frame with the epitope\u2010coding sequence, avoiding longer procedures such as site\u2010directed mutagenesis. This heterologous construction can be realized either at the 5\u2032\u2010end of the coding sequence, in the pYeF1 vector, or at its 3\u2032\u2010end, in pYeF2, generating N\u2010 or C\u2010terminal tagged proteins, respectively. Moreover, to increase the usefulness of the method, derivatives of the two basic <jats:italic>URA3<\/jats:italic>\u2010borne pYeF1 and pYeF2 were constructed, carrying either the <jats:italic>HIS3<\/jats:italic> or <jats:italic>TRP1<\/jats:italic> gene as a marker of selection. These vectors could be of use for the purpose of functional analysis of the newly discovered genes resulting from the systematic sequencing of the yeast genome. Here, we present results showing the functional expression and the efficient immunoprecipitation of the epitope\u2010tagged Rna15 protein, which is involved in <jats:italic>Saccharomyces cerevisiae<\/jats:italic> mRNA stability.<\/jats:p>","DOI":"10.1002\/yea.320100110","type":"journal-article","created":{"date-parts":[[2005,5,28]],"date-time":"2005-05-28T23:46:04Z","timestamp":1117323964000},"page":"105-112","source":"Crossref","is-referenced-by-count":60,"title":["Multipurpose vectors designed for the fast generation of N\u2010 or C\u2010terminal epitope\u2010tagged proteins"],"prefix":"10.1002","volume":"10","author":[{"given":"Christophe","family":"Cullin","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lionel","family":"Minvielle\u2010Sebastia","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,1,29]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.10.12.6417"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(86)90065-6"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00330984"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0076-6879(87)54076-9"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/yea.320070609"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-291X(05)80051-8"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.8.5.2159"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/20.6.1425"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.79.23.7410"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/yea.320020304"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(92)90137-E"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.9.12.5387"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/0076-6879(91)94038-E"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/227680a0"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.88.23.10485"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.11.6.3075"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/357038a0"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.89.4.1249"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00352019"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1990.tb19483.x"},{"key":"e_1_2_1_22_1","volume-title":"Methods in Yeast Genetics. 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