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Here we describe the isolation of the 3\u2010oxoacyl\u2010CoA thiolase of the peroxisomal \u03b2\u2010oxidation system from <jats:italic>Saccharomyces cerevisiae<\/jats:italic> as a dimer of identical subunits, each with a molecular mass of 45 kDa. Monospecific polyclonal antibodies were raised against the purified enzyme, and its peroxisomal origin was demonstrated by immunoblotting of subcellular fractions as well as by immunogold labelling. We also show that these antibodies could be suitable for an immunofluorescence microscopy screening of yeast mutants affected in peroxisome assembly.<\/jats:p>","DOI":"10.1002\/yea.320100905","type":"journal-article","created":{"date-parts":[[2005,5,28]],"date-time":"2005-05-28T23:49:49Z","timestamp":1117324189000},"page":"1173-1182","source":"Crossref","is-referenced-by-count":47,"title":["Purification and immunolocalization of the peroxisomal 3\u2010oxoacyl\u2010coA thiolase from <i>Saccharomyces cerevisiae<\/i>"],"prefix":"10.1002","volume":"10","author":[{"given":"Ralf","family":"Erdmann","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wolf\u2010Hubert","family":"Kunau","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,1,29]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(76)90527-3"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/yea.320060202"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00312854"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.86.14.5419"},{"key":"e_1_2_1_6_1","unstructured":"Erdmann R.(1989). 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