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We have designed vectors containing the <jats:italic>URA3<\/jats:italic> gene flanked by direct repeats of epitope tags. We use the polymerase chain reaction (PCR) to amplify the tag\u2010<jats:italic>URA3<\/jats:italic>\u2010tag cassette such that the ends of the PCR fragments possess homology to the gene of interest. <jats:italic>In vivo<\/jats:italic> recombination is then used to direct integration of the fragment to the location of interest, and transformants are selected by their Ura<jats:sup>+<\/jats:sup> phenotype. Finally, selection for Ura<jats:sup>\u2212<\/jats:sup> cells on 5\u2010fluoro\u2010orotic acid plates yields cells where recombination between the repeated epitopes has \u2018popped out\u2019 the <jats:italic>URA3<\/jats:italic> gene, leaving a single copy of the epitope at the desired location. PCR epitope tagging (PET) provides a rapid and direct technique for tagging that does not require any cloning steps. We have used PET to tag three <jats:italic>Saccharomyces cerevisiae<\/jats:italic> proteins, Cln1, Sic1 and Est1.<\/jats:p>","DOI":"10.1002\/yea.320111306","type":"journal-article","created":{"date-parts":[[2005,5,28]],"date-time":"2005-05-28T23:53:21Z","timestamp":1117324401000},"page":"1265-1274","source":"Crossref","is-referenced-by-count":289,"title":["Use of polymerase chain reaction epitope tagging for protein tagging in <i>Saccharomyces cerevisiae<\/i>"],"prefix":"10.1002","volume":"11","author":[{"given":"B. L.","family":"Schneider","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W.","family":"Seufert","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B.","family":"Steiner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Q. 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