{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T18:33:02Z","timestamp":1778610782467,"version":"3.51.4"},"reference-count":25,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2019,11,6]],"date-time":"2019-11-06T00:00:00Z","timestamp":1572998400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"},{"start":{"date-parts":[[2019,11,6]],"date-time":"2019-11-06T00:00:00Z","timestamp":1572998400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["41706145"],"award-info":[{"award-number":["41706145"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["41966004"],"award-info":[{"award-number":["41966004"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Key Research and Development Programs of Guangxi","award":["2018AB52003"],"award-info":[{"award-number":["2018AB52003"]}]},{"name":"Natural Science Foundation of Guangxi","award":["2019JJD130019"],"award-info":[{"award-number":["2019JJD130019"]}]},{"name":"Natural Science Foundation of Guangxi","award":["2019JJB130070"],"award-info":[{"award-number":["2019JJB130070"]}]},{"name":"Natural Science Foundation of Guangxi","award":["2019JJB130080"],"award-info":[{"award-number":["2019JJB130080"]}]},{"name":"Basic Research Fund of the Guangxi Academy of Sciences","award":["2018YJJ903"],"award-info":[{"award-number":["2018YJJ903"]}]},{"name":"Basic Research Fund of the Guangxi Academy of Sciences","award":["2018YJJ902"],"award-info":[{"award-number":["2018YJJ902"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["41706145"],"award-info":[{"award-number":["41706145"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["41966004"],"award-info":[{"award-number":["41966004"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["afspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2019,12,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>An outbreak of suspected iridovirus disease in cultured hybrid grouper (\u2640Tiger Grouper Epinephelus fuscoguttatus \u00d7 \u2642 Giant Grouper Epinephelus lanceolatus) occurred in the Guangxi Province in July, 2018. In this study, grouper iridovirus Guangxi (SGIV-Gx) was isolated from diseased hybrid grouper that were collected from Guangxi. Cytopathic effects were observed and identified in grouper spleen cells that were incubated with diseased tissue homogenates after 24 h, and the effects increased at 48 h postinfection. The transmission electron microscopy results showed that viral particles that were about 200 nm in diameter with hexagonal profiles were present in the cell cytoplasm of suspected virus-infected cells. The presence of SGIV-Gx (accession number: MK107821) was identified by polymerase chain reaction (PCR) and amplicon sequencing, which showed that this strain was most closely related to Singapore grouper iridovirus (AY521625.1). The detection of SGIV-Gx infection was further supported by novel aptamer (Q2c)-based detection technology. The effects of temperature and pH on viral infectivity were analyzed by using reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR) and cell culture. The results indicated that SGIV-Gx was resistant to exposure to pH levels 5, 7, and 7.5 for 1 h, but its infectivity was remarkably lower at pH levels 3 and 10 after 1 h. The analyses showed that SGIV-Gx was stable for 1 h at 4\u00b0C and 25\u00b0C but was inactivated after 1 h at 40, 50, and 60\u00b0C.<\/jats:p>","DOI":"10.1002\/aah.10090","type":"journal-article","created":{"date-parts":[[2019,9,13]],"date-time":"2019-09-13T21:49:28Z","timestamp":1568411368000},"page":"364-370","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":39,"title":["Isolation and Characterization of a Ranavirus Associated with Disease Outbreaks in Cultured Hybrid Grouper (\u2640 Tiger Grouper <i>Epinephelus fuscoguttatus<\/i> \u00d7 \u2642 Giant Grouper <i>E. lanceolatus<\/i>) in Guangxi, China"],"prefix":"10.1093","volume":"31","author":[{"given":"Hehe","family":"Xiao","sequence":"first","affiliation":[{"name":"College of Life Science, Henan Normal University, Xinxiang, China; and Guangxi Key Laboratory of Marine Environmental Science, Guangxi Academy of Sciences, Nanning, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mingzhu","family":"Liu","sequence":"additional","affiliation":[{"name":"Guangxi Key Laboratory for Marine Biotechnology, Guangxi Institute of Oceanography, Guangxi Academy of Sciences, Beihai, China; and Guangxi Key Laboratory of Beibu Gulf Marine Biodiversity Conservation, College of Marine Sciences, Beibu Gulf University, Qinzhou, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Siqiao","family":"Li","sequence":"additional","affiliation":[{"name":"Guangxi Key Laboratory of Marine Environmental Science, Guangxi Academy of Sciences, Nanning, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Deqiang","family":"Shi","sequence":"additional","affiliation":[{"name":"Guangxi Key Laboratory of Marine Environmental Science, Guangxi Academy of Sciences, Nanning, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Donglin","family":"Zhu","sequence":"additional","affiliation":[{"name":"Guangxi Key Laboratory of Marine Environmental Science, Guangxi Academy of Sciences, Nanning, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ke","family":"Ke","sequence":"additional","affiliation":[{"name":"Guangxi Key Laboratory of Marine Environmental Science, Guangxi Academy of Sciences, Nanning, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Youhou","family":"Xu","sequence":"additional","affiliation":[{"name":"Guangxi Key Laboratory of Beibu Gulf Marine Biodiversity Conservation, College of Marine Sciences, Beibu Gulf University, Qinzhou, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dexin","family":"Dong","sequence":"additional","affiliation":[{"name":"Guangxi Key Laboratory of Marine Environmental Science, Guangxi Academy of Sciences, Nanning, 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