{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T06:41:59Z","timestamp":1778568119502,"version":"3.51.4"},"reference-count":30,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2021,6,14]],"date-time":"2021-06-14T00:00:00Z","timestamp":1623628800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"},{"start":{"date-parts":[[2021,6,14]],"date-time":"2021-06-14T00:00:00Z","timestamp":1623628800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["afspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2021,9,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Among the bacterial infections that impair the health status of marine mammals, those caused by Brucella spp. are the most reported worldwide. Brucella infections in marine mammals can result in acute or chronic disease and are associated with variable clinical outcomes, depending on the organ involved during the infectious process, infection route, host immunity, and strain pathogenicity. Asymptomatic infections may also occur. The current study expands the investigation of Brucella infection in northeast Brazil by analyzing 19 dead, stranded cetaceans and 52 Antillean manatees Trichechus manatus manatus. The manatees included 8 dead, captive manatees and 44 live specimens, of which 10 were analyzed only after reintroduction into the wild as part of a rehabilitation program, 9 were analyzed both while in captivity or semi-captivity and after reintroduction, 20 were sampled only in captivity or semi-captivity, and 5 were free-living manatees. Serological tests were used to screen for antibodies against smooth Brucella spp. Whole blood, swabs, and tissue samples were screened for Brucella spp. DNA by PCR. Samples with positive PCR results were cultured for Brucella spp. isolation. All manatees yielded negative results in serological and molecular tests. Brucella spp. DNA was detected in the kidney of one adult Guiana dolphin Sotalia guianensis exhibiting necrosis in the liver. No growth of Brucella spp. was observed via microbiological culturing. This study is the first report of Brucella spp. DNA detection in cetaceans in the state of Pernambuco, and it highlights the importance of conducting systematic monitoring for the presence of Brucella infection in marine mammals along the Brazilian coast, especially in the northeast region, where several cases have been reported.<\/jats:p>","DOI":"10.1002\/aah.10129","type":"journal-article","created":{"date-parts":[[2021,6,14]],"date-time":"2021-06-14T08:23:49Z","timestamp":1623659029000},"page":"125-132","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["<i>Brucella<\/i> Infection Investigation in Cetaceans and Manatees in Northeast Brazil"],"prefix":"10.1093","volume":"33","author":[{"given":"Gl\u00e1ucia Pereira","family":"de Sousa","sequence":"first","affiliation":[{"name":"Departamento de Medicina Veterin\ufffdria Preventiva e Sa\ufffdde Animal, Faculdade de Medicina Veterin\ufffdria e Zootecnia, Universidade de S\ufffdo Paulo, Pirassununga, S\ufffdo Paulo 13635-900, Brazil; and Centro Nacional de Pesquisa e Conserva\ufffd\ufffdo de Mam\ufffdferos Aqu\ufffdticos, Instituto Chico Mendes de Conserva\ufffd\ufffdo da Biodiversidade, Santos, S\ufffdo Paulo 11050-031, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rodrigo Martins","family":"Soares","sequence":"additional","affiliation":[{"name":"Departamento de Medicina Veterin\u00e1ria Preventiva e Sa\u00fade Animal, Faculdade de Medicina Veterin\u00e1ria e Zootecnia, Universidade de S\u00e3o Paulo, Pirassununga, S\u00e3o Paulo, 13635-900, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jo\u00e3o Carlos Gomes","family":"Borges","sequence":"additional","affiliation":[{"name":"Funda\ufffd\ufffdo Mam\ufffdferos Aqu\ufffdticos, Recife, Pernambuco 52051-305, Brazil; and Programa de P\ufffds-Gradua\ufffd\ufffdo em Ecologia e Monitoramento Ambiental, Universidade Federal da Para\ufffdba, Rio Tinto, Para\ufffdba 58297-000, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana Paula Domingos","family":"Brito","sequence":"additional","affiliation":[{"name":"Centro Nacional de Pesquisa e Conserva\u00e7\u00e3o da Biodiversidade Marinha do Nordeste, Instituto Chico Mendes de Conserva\u00e7\u00e3o da Biodiversidade, Ilha de Itamarac\u00e1, Pernambuco, 53900-000, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniella Carvalho Ribeiro","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Departamento de Medicina Veterin\u00e1ria Preventiva e Sa\u00fade Animal, Faculdade de Medicina Veterin\u00e1ria e Zootecnia, Universidade de S\u00e3o Paulo, Pirassununga, S\u00e3o Paulo, 13635-900, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thalita","family":"Faita","sequence":"additional","affiliation":[{"name":"Departamento de Medicina Veterin\u00e1ria Preventiva e Sa\u00fade Animal, Faculdade de Medicina Veterin\u00e1ria e Zootecnia, Universidade de S\u00e3o Paulo, Pirassununga, S\u00e3o Paulo, 13635-900, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fernanda Loffler Niemeyer","family":"Attademo","sequence":"additional","affiliation":[{"name":"Projeto Cet\ufffdceos da Costa Branca, Universidade do Estado do Rio Grande do Norte, Natal, Rio Grande do Norte 59080-590, Brazil; and Centro Nacional de Pesquisa e Conserva\ufffd\ufffdo de Mam\ufffdferos Aqu\ufffdticos, Instituto Chico Mendes de Conserva\ufffd\ufffdo da Biodiversidade, Ilha de Itamarac\ufffd, Pernambuco 53900-000, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F\u00e1bia de Oliveira","family":"Luna","sequence":"additional","affiliation":[{"name":"Centro Nacional de Pesquisa e Conserva\u00e7\u00e3o de Mam\u00edferos Aqu\u00e1ticos, Instituto Chico Mendes de Conserva\u00e7\u00e3o da Biodiversidade, Santos, S\u00e3o Paulo, 11050-031, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0370-4447","authenticated-orcid":false,"given":"Radan Elvis Matias","family":"de Oliveira","sequence":"additional","affiliation":[{"name":"Projeto Cet\ufffdceos da Costa Branca, Universidade do Estado do Rio Grande do Norte, Natal, Rio Grande do Norte 59080-590, Brazil; and Programa de P\ufffds-Gradua\ufffd\ufffdo em Ci\ufffdncia Animal, Universidade Federal Rural do Semi-\ufffdrido, Mossor\ufffd, Rio Grande do Norte 59625-900, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carlos Iber\u00ea Alves","family":"Freitas","sequence":"additional","affiliation":[{"name":"Departamento de Ci\u00eancias da Sa\u00fade, Universidade Federal Rural do Semi-\u00c1rido, Mossor\u00f3, Rio Grande do Norte, 59625-900, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jociery Einhardt","family":"Vergara-Parente","sequence":"additional","affiliation":[{"name":"Funda\u00e7\u00e3o Mam\u00edferos Aqu\u00e1ticos, Recife, Pernambuco, 52051-305, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lara Borges","family":"Keid","sequence":"additional","affiliation":[{"name":"Departamento de Medicina Veterin\u00e1ria, Faculdade de Zootecnia e Engenharia de Alimentos, Universidade de S\u00e3o Paulo, Pirassununga, S\u00e3o Paulo, 13635-900, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2021,6,14]]},"reference":[{"key":"2025011310211075200_aah10129-bib-0001","doi-asserted-by":"crossref","first-page":"158","DOI":"10.1016\/j.vetmic.2013.01.023","article-title":"The presence of Brucella ceti ST26 in a striped dolphin (Stenella coeruleoalba) with meningoencephalitis from the Mediterranean Sea","volume":"164","author":"Alba","year":"2013","journal-title":"Veterinary 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