{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T17:48:39Z","timestamp":1791395319600,"version":"4.3.3"},"reference-count":43,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2022,9,1]],"date-time":"2022-09-01T00:00:00Z","timestamp":1661990400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"},{"start":{"date-parts":[[2022,9,27]],"date-time":"2022-09-27T00:00:00Z","timestamp":1664236800000},"content-version":"vor","delay-in-days":26,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001724","name":"International Foundation for Science","doi-asserted-by":"publisher","award":["I2-A-6542-1"],"award-info":[{"award-number":["I2-A-6542-1"]}],"id":[{"id":"10.13039\/501100001724","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001724","name":"International Foundation for Science","doi-asserted-by":"publisher","award":["I2\u2010A\u20106542\u20101"],"award-info":[{"award-number":["I2\u2010A\u20106542\u20101"]}],"id":[{"id":"10.13039\/501100001724","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["afspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2022,9,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Production of Nile Tilapia Oreochromis niloticus contributes to economic growth in many countries. However, there has been a decline in its production over the years due to the influx of bacterial infections, with Aeromonas jandaei as an emerging threat. In this study, we identified and characterized A. jandaei from cage-cultured Nile Tilapia in Akosombo Stratum II of Lake Volta in Ghana and evaluated its response to commonly used antibiotics using the disc diffusion and agar well diffusion methods for herbal extracts at various concentrations (10, 30, 50, 70, and 100\u2009mg\/mL). The herbs considered included guava Psidium guajava leaf, bitter leaf Vernonia amygdalina, neem Azadirachta indica leaf, and their cocktail (GBNL in the ratio of 1:1:1). The bacterium was isolated from swab samples from the head kidneys of 27 moribund Nile Tilapia collected from nine fish farms. Samples were screened for A. jandaei by culturing and identification using morphological and molecular techniques. The bacterium isolate from fish in the study, identified as A. jandaei GH-AS II, had 92\u201393% identity to A. jandaei reference strains. Infection of healthy Nile Tilapia (n\u2009=\u2009210) with the bacterium isolate showed that 1.0\u2009\u00d7\u2009105\u2009CFU\/mL was the lethal dose causing 50% mortality. Antibiotic susceptibility testing showed that A. jandaei GH-AS II was resistant to tetracycline and ampicillin. Herbal extracts at the various concentrations inhibited the growth of the bacterium isolate, with a significant increment in the zones of inhibition with increasing concentrations of leaf extracts. However, GBNL showed prominence compared to the other extracts only at 100\u2009mg\/mL. Management of A. jandaei GH-AS II by using herbal extracts at Nile Tilapia farms in Lake Volta may be recommended since the use of antibiotics, such as tetracycline and ampicillin, may not yield the needed result.<\/jats:p>","DOI":"10.1002\/aah.10165","type":"journal-article","created":{"date-parts":[[2022,9,27]],"date-time":"2022-09-27T12:47:23Z","timestamp":1664282843000},"page":"140-148","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Isolation and Characterization of <i>Aeromonas jandaei<\/i> from Nile Tilapia in Lake Volta, Ghana, and Its Response to Antibiotics and Herbal Extracts"],"prefix":"10.1093","volume":"34","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4873-6546","authenticated-orcid":false,"given":"E. D.","family":"Abarike","sequence":"first","affiliation":[{"name":"Department of Fisheries and Aquatic Resources Management, University for Development Studies, Tamale, Ghana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R. A.","family":"Atuna","sequence":"additional","affiliation":[{"name":"Department of Food Science and Technology, University for Development Studies, Tamale, Ghana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Agyekum","sequence":"additional","affiliation":[{"name":"Department of Fisheries and Aquatic Resources Management, University for Development Studies, Tamale, Ghana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D. N.","family":"Akongyuure","sequence":"additional","affiliation":[{"name":"Department of Fisheries and Aquatic Resources Management, University for Development Studies, Tamale, Ghana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E. H.","family":"Alhassan","sequence":"additional","affiliation":[{"name":"Department of Fisheries and Aquatic Resources Management, University for Development Studies, Tamale, Ghana"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2022,9,27]]},"reference":[{"key":"2025011310210764000_aah10165-bib-0001","doi-asserted-by":"crossref","first-page":"2366","DOI":"10.1111\/are.13691","article-title":"Influence of traditional Chinese medicine and Bacillus species (TCMBS) on growth, immune response and disease resistance in Nile Tilapia, Oreochromis niloticus","volume":"49","author":"Abarike","year":"2018","journal-title":"Aquaculture Research"},{"key":"2025011310210764000_aah10165-bib-0002","first-page":"6641461","article-title":"Multiple antibiotic resistance-coliform bacteria in some selected fish farms of the central region of Ghana","volume":"2020","author":"Adinortey","year":"2020","journal-title":"Hindawi Scientifica"},{"issue":"2","key":"2025011310210764000_aah10165-bib-0003","doi-asserted-by":"crossref","first-page":"9","DOI":"10.21767\/2472-1093.100036","article-title":"Antibiotic use and practices in selected fish farms in the Ashanti region of Ghana","volume":"3","author":"Agoba","year":"2017","journal-title":"Journal of Infectious Diseases and Treatment"},{"issue":"8","key":"2025011310210764000_aah10165-bib-0004","doi-asserted-by":"crossref","DOI":"10.4172\/2155-9546.1000545","article-title":"A review of Ghana's aquaculture industry","volume":"9","author":"Amenyogbe","year":"2018","journal-title":"Journal of Aquaculture Research and Development"},{"key":"2025011310210764000_aah10165-bib-0005","doi-asserted-by":"crossref","first-page":"314","DOI":"10.5897\/AJPP2017.4789","article-title":"Antibiotic sensitivity patterns of microbial isolates from fish ponds: a study in the Greater Accra Region of Ghana","volume":"11","author":"Apenteng","year":"2017","journal-title":"African Journal of Pharmacy and Pharmacology"},{"key":"2025011310210764000_aah10165-bib-0006","doi-asserted-by":"crossref","first-page":"e276","DOI":"10.1111\/tbed.14295","article-title":"Enterogyrus spp. (Monogenea: Ancyrocephalinae) and Aeromonas jandaei co-infection associated with high mortality following transport stress in cultured Nile Tilapia","volume":"69","author":"Assane","year":"2022","journal-title":"Transboundary and Emerging Diseases"},{"key":"2025011310210764000_aah10165-bib-0007","doi-asserted-by":"crossref","first-page":"355","DOI":"10.3923\/ajava.2013.355.361","article-title":"Antibacterial activity of neem (Azadirachta indica) leaves on Vibrio spp. isolated from cultured shrimp. 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