{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T21:19:03Z","timestamp":1789766343402,"version":"4.0.1"},"reference-count":53,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2024,12,1]],"date-time":"2024-12-01T00:00:00Z","timestamp":1733011200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"},{"start":{"date-parts":[[2024,12,1]],"date-time":"2024-12-01T00:00:00Z","timestamp":1733011200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["afspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2024,12,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Objective<\/jats:title>\n                  <jats:p>The choice of a stable reference gene plays a crucial role in gene expression analysis. In most cases, the choice of housekeeping gene (HKG) is quite random. However, the expression of HKGs varies under different experimental conditions and in different tissues.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>The study focused on three reference genes in Catla Labeo catla\u2014beta actin (\u03b2-actin), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), and elongation factor-1 alpha (EF-1\u03b1)\u2014under four experimental conditions: bacterial challenge, hypoxia, a combination of bacterial challenge and hypoxia, and control. The fish were subjected to the above-mentioned experimental circumstances, and five tissues (blood, gill, liver, kidney, and spleen) were collected at regular intervals.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Result<\/jats:title>\n                  <jats:p>The investigation of gene expression in the five tissues was performed using real-time (RT) quantitative polymerase chain reaction (qPCR), and the resultant cycle threshold values were subjected to several statistical techniques via the RefFinder tool to determine the most stable HKG. The results were ordered in accordance with the stability of the reference genes. In this study, GAPDH was found to be the least stable gene, while \u03b2-actin and EF-1\u03b1 remained stable in the kidney and liver under various circumstances. Other tissues demonstrated tissue- and treatment-specific stability for the various HKGs.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusion<\/jats:title>\n                  <jats:p>The findings indicate that GAPDH should be avoided in Catla for gene expression studies and that different algorithms should be employed to validate the stability of HKGs in RT-qPCR analysis.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title\/>\n               <\/jats:sec>","DOI":"10.1002\/aah.10234","type":"journal-article","created":{"date-parts":[[2024,12,2]],"date-time":"2024-12-02T05:09:33Z","timestamp":1733116173000},"page":"330-341","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Biotic and abiotic stress affects the stability of housekeeping genes in Catla <i>Labeo catla<\/i>"],"prefix":"10.1093","volume":"36","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7535-7341","authenticated-orcid":false,"given":"Arpit","family":"Acharya","sequence":"first","affiliation":[{"name":"Indian Council of Agricultural Research, Central Institute of Fisheries Education, Mumbai, Maharashtra, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1230-2618","authenticated-orcid":false,"given":"Gayatri","family":"Tripathi","sequence":"additional","affiliation":[{"name":"Indian Council of Agricultural Research, Central Institute of Fisheries Education, Mumbai, Maharashtra, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kiran D.","family":"Rasal","sequence":"additional","affiliation":[{"name":"Indian Council of Agricultural Research, Central Institute of Fisheries Education, Mumbai, Maharashtra, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mujahidkhan A.","family":"Pathan","sequence":"additional","affiliation":[{"name":"Indian Council of Agricultural Research, Central Institute of Fisheries Education, Mumbai, Maharashtra, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raja Aadil Hussain","family":"Bhat","sequence":"additional","affiliation":[{"name":"Indian Council of Agricultural Research, Directorate of Coldwater Fisheries and Research, Bhimtal, Uttarakhand, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chandana","family":"Dinakaran","sequence":"additional","affiliation":[{"name":"Indian Council of Agricultural Research, Central Institute of Fisheries Education, Mumbai, Maharashtra, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R. Bharathi","family":"Rathinam","sequence":"additional","affiliation":[{"name":"Indian Council of Agricultural Research, Central Institute of Fisheries Education, Mumbai, Maharashtra, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2024,12,1]]},"reference":[{"key":"2025012213245126700_aah10234-bib-0001","doi-asserted-by":"crossref","first-page":"997","DOI":"10.1007\/s10695-019-00614-9","article-title":"Fish response to hypoxia stress: Growth, physiological, and immunological biomarkers","volume":"45","author":"Abdel-Tawwab","year":"2019","journal-title":"Fish Physiology and Biochemistry"},{"issue":"15","key":"2025012213245126700_aah10234-bib-0002","doi-asserted-by":"crossref","first-page":"5245","DOI":"10.1158\/0008-5472.CAN-04-0496","article-title":"Normalization of real-time quantitative reverse transcription-PCR data: A model-based variance estimation approach to identify genes suited for normalization, applied to bladder and colon cancer data sets","volume":"64","author":"Andersen","year":"2004","journal-title":"Cancer Research"},{"key":"2025012213245126700_aah10234-bib-0003","doi-asserted-by":"crossref","first-page":"119071","DOI":"10.1016\/j.gep.2019.119071","article-title":"Ontogeny and tissue-specific expression of immune-relevant genes in Catla catla (Hamilton)","volume":"34","author":"Arya","year":"2019","journal-title":"Gene Expression Patterns"},{"key":"2025012213245126700_aah10234-bib-0004","doi-asserted-by":"crossref","first-page":"682136","DOI":"10.3389\/fviro.2021.682136","article-title":"Gapdh, interferon \u03b3, and nitric oxide: Inhibitors of coronaviruses","volume":"1","author":"Awan","year":"2021","journal-title":"Frontiers in Virology"},{"key":"2025012213245126700_aah10234-bib-0005","doi-asserted-by":"crossref","first-page":"Article 80","DOI":"10.1186\/1471-2199-10-80","article-title":"Selection of reference genes for expression studies with fish myogenic cell cultures","volume":"10","author":"Bower","year":"2009","journal-title":"BMC Molecular Biology"},{"key":"2025012213245126700_aah10234-bib-0006","doi-asserted-by":"crossref","first-page":"1540","DOI":"10.1007\/s12035-015-9114-5","article-title":"Normalization of reverse transcription quantitative PCR data during ageing in distinct cerebral structures","volume":"53","author":"Bruckert","year":"2016","journal-title":"Molecular Neurobiology"},{"issue":"9","key":"2025012213245126700_aah10234-bib-0007","doi-asserted-by":"crossref","first-page":"1808","DOI":"10.1093\/molbev\/msl049","article-title":"Gene loss and evolutionary rates following whole-genome duplication in teleost fishes","volume":"23","author":"Brunet","year":"2006","journal-title":"Molecular Biology and Evolution"},{"issue":"1","key":"2025012213245126700_aah10234-bib-0008","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1677\/jme.0.0290023","article-title":"Quantification of mRNA using real-time reverse transcription PCR (RT-PCR): Trends and problems","volume":"29","author":"Bustin","year":"2002","journal-title":"Journal of Molecular Endocrinology"},{"issue":"4","key":"2025012213245126700_aah10234-bib-0009","doi-asserted-by":"crossref","first-page":"611","DOI":"10.1373\/clinchem.2008.112797","article-title":"The MIQE guidelines: Minimum information for publication of quantitative real-time PCR experiments","volume":"55","author":"Bustin","year":"2009","journal-title":"Clinical Chemistry"},{"issue":"3","key":"2025012213245126700_aah10234-bib-0010","doi-asserted-by":"crossref","first-page":"597","DOI":"10.1677\/jme.1.01755","article-title":"Quantitative real-time RT-PCR\u2013a perspective","volume":"34","author":"Bustin","year":"2005","journal-title":"Journal of Molecular Endocrinology"},{"issue":"4","key":"2025012213245126700_aah10234-bib-0011","doi-asserted-by":"crossref","first-page":"399","DOI":"10.1016\/j.marenvres.2007.01.007","article-title":"Hypoxia in the East China Sea: One of the largest coastal low-oxygen areas in the world","volume":"64","author":"Chen","year":"2007","journal-title":"Marine Environmental Research"},{"key":"2025012213245126700_aah10234-bib-0012","doi-asserted-by":"crossref","first-page":"110270","DOI":"10.1016\/j.vetimm.2021.110270","article-title":"Evaluation of candidate reference genes for quantitative RTqPCR analysis in Goldfish (Carassius auratus L.) in healthy and CyHV-2 infected fish","volume":"237","author":"Dharmaratnam","year":"2021","journal-title":"Veterinary Immunology and Immunopathology"},{"issue":"1","key":"2025012213245126700_aah10234-bib-0013","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1016\/j.ab.2005.05.022","article-title":"The implications of using an inappropriate reference gene for real-time reverse transcription PCR data normalization","volume":"344","author":"Dheda","year":"2005","journal-title":"Analytical Biochemistry"},{"key":"2025012213245126700_aah10234-bib-0014","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1251\/bpo114","article-title":"Rapid and simple comparison of messenger RNA levels using real-time PCR","volume":"8","author":"Dussault","year":"2006","journal-title":"Biological Procedures Online"},{"issue":"4","key":"2025012213245126700_aah10234-bib-0015","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1080\/10641260500320845","article-title":"Modern trends in Aeromonas hydrophila disease management with fish","volume":"13","author":"Harikrishnan","year":"2005","journal-title":"Reviews in Fisheries Science"},{"key":"2025012213245126700_aah10234-bib-0016","doi-asserted-by":"crossref","first-page":"1721","DOI":"10.1007\/s10695-014-9962-3","article-title":"Reference gene selection for real-time RT-PCR normalization in Rice Field Eel (Monopterus albus) during gonad development","volume":"40","author":"Hu","year":"2014","journal-title":"Fish Physiology and Biochemistry"},{"issue":"8","key":"2025012213245126700_aah10234-bib-0017","doi-asserted-by":"crossref","first-page":"2258","DOI":"10.3390\/ijms19082258","article-title":"Validation and evaluation of reference genes for quantitative real-time PCR in Macrobrachium nipponense","volume":"19","author":"Hu","year":"2018","journal-title":"International Journal of Molecular Sciences"},{"issue":"8","key":"2025012213245126700_aah10234-bib-0018","doi-asserted-by":"crossref","first-page":"1194","DOI":"10.1016\/j.molimm.2005.07.009","article-title":"Expression profiling and validation of reference gene candidates in immune relevant tissues and cells from Atlantic Salmon (Salmo salar L.)","volume":"43","author":"Ingerslev","year":"2006","journal-title":"Molecular Immunology"},{"key":"2025012213245126700_aah10234-bib-0019","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1007\/BF03260053","article-title":"Housekeeping genes as internal standards in cancer research","volume":"8","author":"Janssens","year":"2004","journal-title":"Molecular Diagnosis"},{"key":"2025012213245126700_aah10234-bib-0020","doi-asserted-by":"crossref","first-page":"398","DOI":"10.1007\/s10126-005-5164-4","article-title":"Validation of reference genes for real-time polymerase chain reaction studies in Atlantic Salmon","volume":"8","author":"Jorgensen","year":"2006","journal-title":"Marine Biotechnology"},{"key":"2025012213245126700_aah10234-bib-0021","doi-asserted-by":"crossref","first-page":"108625","DOI":"10.1016\/j.fsi.2023.108625","article-title":"Abiotic and biotic stress alleviating effects of the medicinal and aromatic plant-derived product on Striped Catfish Pangasianodon hypophthalmus","volume":"135","author":"Kate","year":"2023","journal-title":"Fish & Shellfish Immunology"},{"key":"2025012213245126700_aah10234-bib-0022","doi-asserted-by":"crossref","first-page":"Article 28","DOI":"10.1186\/s41240-019-0142-3","article-title":"Validation of housekeeping genes as candidate internal references for quantitative expression studies in healthy and nervous necrosis virus-infected Seven-band Grouper (Hyporthodus septemfasciatus)","volume":"22","author":"Krishnan","year":"2019","journal-title":"Fisheries and Aquatic Sciences"},{"key":"2025012213245126700_aah10234-bib-0023","doi-asserted-by":"crossref","first-page":"3281","DOI":"10.1007\/s11033-020-05388-5","article-title":"Ontogenetic and tissue-specific expression of gonadotropin-inhibitory hormone (GnIH) and its receptors in Catla catla","volume":"47","author":"Kumar","year":"2020","journal-title":"Molecular Biology Reports"},{"key":"2025012213245126700_aah10234-bib-0024","doi-asserted-by":"crossref","first-page":"993","DOI":"10.1007\/s40011-014-0482-9","article-title":"Characterisation and validation of house keeping gene for expression analysis in Catla catla (Hamilton)","volume":"85","author":"Kumari","year":"2015","journal-title":"Proceedings of the National Academy of Sciences, India Section B: Biological Sciences"},{"key":"2025012213245126700_aah10234-bib-0025","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1016\/j.margen.2013.08.002","article-title":"Selection of reference genes for reverse transcription quantitative real-time PCR normalization in Black Rockfish (Sebastes schlegeli)","volume":"11","author":"Liman","year":"2013","journal-title":"Marine Genomics"},{"issue":"2","key":"2025012213245126700_aah10234-bib-0026","doi-asserted-by":"crossref","first-page":"120","DOI":"10.3390\/fishes8020120","article-title":"Selection of reference gene for expression studies in the ovary and pituitary of Spotted Scat (Scatophagus argus) at different ovarian stages","volume":"8","author":"Liu","year":"2023","journal-title":"Fishes"},{"issue":"4","key":"2025012213245126700_aah10234-bib-0027","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of relative gene expression data using real-time quantitative PCR and the 2\u2212\u0394\u0394CT method","volume":"25","author":"Livak","year":"2001","journal-title":"Methods"},{"issue":"4","key":"2025012213245126700_aah10234-bib-0028","doi-asserted-by":"crossref","first-page":"340","DOI":"10.1016\/j.cbpb.2009.04.009","article-title":"Evaluation of candidate reference genes for QPCR during ontogenesis and of immune-relevant tissues of European Seabass (Dicentrarchus labrax)","volume":"153","author":"Mitter","year":"2009","journal-title":"Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology"},{"key":"2025012213245126700_aah10234-bib-0029","doi-asserted-by":"crossref","DOI":"10.1186\/1471-2199-6-21","article-title":"Evaluation of potential reference genes in real-time RT-PCR studies of Atlantic Salmon","volume":"6","author":"Olsvik","year":"2005","journal-title":"BMC Molecular Biology"},{"key":"2025012213245126700_aah10234-bib-0030","doi-asserted-by":"crossref","DOI":"10.1186\/1471-2199-11-36","article-title":"Evaluation of potential reference genes for real time RT-PCR studies in Atlantic Halibut (Hippoglossus hippoglossus L.); during development, in tissues of healthy and NNV-injected fish, and in anterior kidney leucocytes","volume":"11","author":"\u00d8verg\u00e5rd","year":"2010","journal-title":"BMC Molecular Biology"},{"key":"2025012213245126700_aah10234-bib-0031","doi-asserted-by":"crossref","DOI":"10.1186\/1756-0500-3-101","article-title":"An evaluation of potential reference genes for stability of expression in two salmonid cell lines after infection with either Piscirickettsia salmonis or IPNV","volume":"3","author":"Pe\u00f1a","year":"2010","journal-title":"BMC Research Notes"},{"key":"2025012213245126700_aah10234-bib-0032","doi-asserted-by":"crossref","first-page":"509","DOI":"10.1023\/B:BILE.0000019559.84305.47","article-title":"Determination of stable housekeeping genes, differentially regulated target genes and sample integrity: BestKeeper\u2013Excel-based tool using pair-wise correlations","volume":"26","author":"Pfaffl","year":"2004","journal-title":"Biotechnology Letters"},{"issue":"3","key":"2025012213245126700_aah10234-bib-0033","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1093\/oxfordjournals.aje.a118408","article-title":"A simple method of estimating fifty per cent endpoints","volume":"27","author":"Reed","year":"1938","journal-title":"American Journal of Epidemiology"},{"key":"2025012213245126700_aah10234-bib-0034","doi-asserted-by":"crossref","DOI":"10.1186\/1471-2199-11-27","article-title":"Elasmobranch qPCR reference genes: A case study of hypoxia preconditioned Epaulette Sharks","volume":"11","author":"Rytk\u00f6nen","year":"2010","journal-title":"BMC Molecular Biology"},{"issue":"1\u20132","key":"2025012213245126700_aah10234-bib-0035","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1016\/j.fsi.2008.04.003","article-title":"Family association between immune parameters and resistance to Aeromonas hydrophila infection in the Indian major carp, Labeo rohita","volume":"25","author":"Sahoo","year":"2008","journal-title":"Fish & Shellfish Immunology"},{"issue":"1","key":"2025012213245126700_aah10234-bib-0036","doi-asserted-by":"crossref","first-page":"2660","DOI":"10.1038\/s41598-023-29325-1","article-title":"Stability evaluation and validation of appropriate reference genes for real-time PCR expression analysis of immune genes in the Rohu (Labeo rohita) skin following argulosis","volume":"13","author":"Sahoo","year":"2023","journal-title":"Scientific Reports"},{"key":"2025012213245126700_aah10234-bib-0037","first-page":"1","volume-title":"Biology of stress in fish","author":"Schreck","year":"2016"},{"key":"2025012213245126700_aah10234-bib-0038","doi-asserted-by":"crossref","first-page":"101756","DOI":"10.1016\/j.genrep.2023.101756","article-title":"Which housekeeping gene is the best choice for RT-qPCR analysis in mice fed with a high-fat diet? Studies in the liver, kidney, pancreas, and intestines","volume":"31","author":"Secio-Silva","year":"2023","journal-title":"Gene Reports"},{"key":"2025012213245126700_aah10234-bib-0039","doi-asserted-by":"crossref","DOI":"10.1186\/1471-2199-7-33","article-title":"Selection of housekeeping genes for gene expression studies in human reticulocytes using real-time PCR","volume":"7","author":"Silver","year":"2006","journal-title":"BMC Molecular Biology"},{"issue":"3","key":"2025012213245126700_aah10234-bib-0040","doi-asserted-by":"crossref","first-page":"296","DOI":"10.1016\/j.cbpb.2008.07.010","article-title":"Stability of reference genes for real-time PCR analyses in Channel Catfish (Ictalurus punctatus) tissues under varying physiological conditions","volume":"151","author":"Small","year":"2008","journal-title":"Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology"},{"key":"2025012213245126700_aah10234-bib-0041","doi-asserted-by":"crossref","first-page":"2387","DOI":"10.1007\/s10499-021-00754-x","article-title":"Characterisation and validation of housekeeping genes for qRT-PCR expression analysis in Pterophyllum scalare","volume":"29","author":"Sushila","year":"2021","journal-title":"Aquaculture International"},{"issue":"2","key":"2025012213245126700_aah10234-bib-0042","doi-asserted-by":"crossref","first-page":"332","DOI":"10.2144\/00292rv02","article-title":"Control selection for RNA quantitation","volume":"29","author":"Suzuki","year":"2000","journal-title":"BioTechniques"},{"issue":"5","key":"2025012213245126700_aah10234-bib-0043","doi-asserted-by":"crossref","first-page":"384","DOI":"10.1111\/j.1745-7270.2007.00283.x","article-title":"Validation of Zebrafish (Danio rerio) reference genes for quantitative real-time RT-PCR normalization","volume":"39","author":"Tang","year":"2007","journal-title":"Acta Biochimica et Biophysica Sinica"},{"issue":"4","key":"2025012213245126700_aah10234-bib-0044","doi-asserted-by":"crossref","first-page":"775","DOI":"10.1016\/j.fsi.2012.06.027","article-title":"Identification of housekeeping genes suitable for gene expression analysis in Jian Carp (Cyprinus carpio var. Jian)","volume":"33","author":"Tang","year":"2012","journal-title":"Fish & Shellfish Immunology"},{"key":"2025012213245126700_aah10234-bib-0045","doi-asserted-by":"crossref","first-page":"89","DOI":"10.3390\/antiox13010089","article-title":"Ultrastructural, antioxidant, and metabolic responses of male genetically improved farmed tilapia (GIFT, Oreochromis niloticus) to acute hypoxia stress","volume":"13","author":"Tao","year":"2024","journal-title":"Antioxidants"},{"key":"2025012213245126700_aah10234-bib-0046","doi-asserted-by":"crossref","DOI":"10.1186\/1471-2199-10-17","article-title":"Selection of suitable housekeeping genes for expression analysis in glioblastoma using quantitative RT-PCR","volume":"10","author":"Valente","year":"2009","journal-title":"BMC Molecular Biology"},{"key":"2025012213245126700_aah10234-bib-0047","doi-asserted-by":"crossref","DOI":"10.1186\/gb-2002-3-7-research0034","article-title":"Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes","volume":"3","author":"Vandesompele","year":"2002","journal-title":"Genome Biology"},{"issue":"5","key":"2025012213245126700_aah10234-bib-0048","doi-asserted-by":"crossref","first-page":"9998","DOI":"10.3390\/ijms16059998","article-title":"Evaluation and selection of appropriate reference genes for real-time quantitative PCR analysis of gene expression in Nile Tilapia (Oreochromis niloticus) during vaccination and infection","volume":"16","author":"Wang","year":"2015","journal-title":"International Journal of Molecular Sciences"},{"key":"2025012213245126700_aah10234-bib-0049","doi-asserted-by":"crossref","first-page":"895","DOI":"10.1007\/s12041-017-0845-0","article-title":"Identification of housekeeping genes as references for quantitative real-time RT-PCR analysis in Misgurnus anguillicaudatus","volume":"96","author":"Xia","year":"2017","journal-title":"Journal of Genetics"},{"key":"2025012213245126700_aah10234-bib-0050","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1007\/s11103-012-9885-2","article-title":"miRDeepFinder: A miRNA analysis tool for deep sequencing of plant small RNAs","volume":"80","author":"Xie","year":"2012","journal-title":"Plant Molecular Biology"},{"key":"2025012213245126700_aah10234-bib-0051","doi-asserted-by":"crossref","first-page":"106783","DOI":"10.1016\/j.aquatox.2023.106783","article-title":"Whole genome identification of Olive Flounder (Paralichthys olivaceus) cathepsin genes: Provides insights into its regulation on biotic and abiotic stresses response","volume":"266","author":"Xie","year":"2024","journal-title":"Aquatic Toxicology"},{"issue":"1","key":"2025012213245126700_aah10234-bib-0052","doi-asserted-by":"crossref","first-page":"8","DOI":"10.3390\/fishes8010008","article-title":"Selection and verification of reference genes for gene expression studies in different cell lines of Golden Pompano (Trachinotus ovatus)","volume":"8","author":"Zhao","year":"2022","journal-title":"Fishes"},{"issue":"2","key":"2025012213245126700_aah10234-bib-0053","doi-asserted-by":"crossref","first-page":"638","DOI":"10.1016\/j.fsi.2010.12.014","article-title":"Evaluation of housekeeping genes as references for quantitative real time RT-PCR analysis of gene expression in Japanese Flounder (Paralichthys olivaceus)","volume":"30","author":"Zheng","year":"2011","journal-title":"Fish & Shellfish Immunology"}],"container-title":["Journal of Aquatic Animal Health"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/jaah\/article-pdf\/36\/4\/330\/61489866\/aah10234.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jaah\/article-pdf\/36\/4\/330\/61489866\/aah10234.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,1,22]],"date-time":"2025-01-22T13:25:07Z","timestamp":1737552307000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jaah\/article\/36\/4\/330\/7959844"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,12,1]]},"references-count":53,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2024,12,1]]}},"URL":"https:\/\/doi.org\/10.1002\/aah.10234","relation":{},"ISSN":["0899-7659","1548-8667"],"issn-type":[{"value":"0899-7659","type":"print"},{"value":"1548-8667","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2024,12,1]]},"published":{"date-parts":[[2024,12,1]]},"assertion":[{"value":"2024-04-07","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-09-05","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-12-01","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}