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We have previously shown that embryonic stem cell\u2010derived motoneurons transplanted at the time of transection into a peripheral nerve can functionally reinnervate muscle. For clinical relevance, we now focused on delaying transplantation to assess reinnervation after prolonged denervation.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Embryonic stem cell\u2010derived motoneurons were transplanted into the distal segments of transected tibial nerves in adult mice after prolonged denervation of 1\u20138 weeks. Twitch and tetanic forces were measured ex vivo 3 months posttransplantation. Tissue was harvested from the transplants for culture and immunohistochemical analysis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>In this delayed reinnervation model, teratocarcinomas developed in about one half of transplants. A residual multipotent cell population (~ 6% of cells) was found despite neural differentiation. Exposure to the alkylating drug mitomycin C eliminated this multipotent population in vitro while preserving motoneurons. Treating neural differentiated stem cells prior to delayed transplantation prevented tumor formation and resulted in twitch and tetanic forces similar to those in animals transplanted acutely after denervation.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Interpretation<\/jats:title>\n                    <jats:p>Despite a neural differentiation protocol, embryonic stem cell\u2010derived motoneurons still carry a risk of tumorigenicity. Pretreating with an antimitotic agent leads to survival and functional muscle reinnervation if performed within 4 weeks of denervation in the mouse.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/acn3.327","type":"journal-article","created":{"date-parts":[[2016,7,1]],"date-time":"2016-07-01T06:55:34Z","timestamp":1467356134000},"page":"637-649","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Tumor prevention facilitates delayed transplant of stem cell\u2010derived motoneurons"],"prefix":"10.1002","volume":"3","author":[{"given":"Philippe","family":"Magown","sequence":"first","affiliation":[{"name":"Medical Neuroscience Dalhousie University Halifax Nova Scotia Canada"},{"name":"Department of Surgery (Neurosurgery) Dalhousie University Halifax Nova Scotia Canada B3H 4R2"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert 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