{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T03:42:57Z","timestamp":1785469377429,"version":"3.56.0"},"reference-count":37,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2019,8,1]],"date-time":"2019-08-01T00:00:00Z","timestamp":1564617600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Ann Clin Transl Neurol"],"published-print":{"date-parts":[[2019,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Exercise training (ET) has beneficial effects on multiple sclerosis and its animal model experimental autoimmune encephalomyelitis (EAE). However, the intensity\u2010dependent effects of ET on the systemic immune system in EAE remain undefined.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>(1) To compare the systemic immune modulatory effects of moderate versus high\u2010intensity ET protocols in protecting against development of EAE; (2) To investigate whether ET affects autoimmunity selectively, or causes general immunosuppression.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Healthy mice performed moderate or high\u2010intensity treadmill running programs. Proteolipid protein (PLP)\u2010induced transfer EAE was utilized to examine ET effects specifically on the systemic immune system. Lymph node (LN)\u2010T cells from trained versus sedentary donor mice were transferred to na\u00efve recipients and EAE severity was assessed, by clinical assessment and histopathological analysis. LN\u2010T cells derived from donor trained versus sedentary PLP\u2010immunized mice were analyzed in vitro for proliferation assays by flow cytometry analysis and cytokine and chemokine receptor gene expression using real\u2010time PCR. T cell\u2010dependent immune responses of trained versus sedentary mice to the nonautoantigen ovalbumin and susceptibility to <jats:italic>Escherichia coli<\/jats:italic>\u2010induced acute peritonitis were examined.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>High\u2010intensity training in healthy donor mice induced significantly greater inhibition than moderate\u2010intensity training on proliferation and generation of encephalitogenic T cells in response to PLP\u2010immunization, and on EAE severity upon their transfer into recipient mice. High\u2010intensity training also inhibited LN\u2010T cell proliferation in response to ovalbumin immunization. <jats:italic>E.<\/jats:italic>\u00a0<jats:italic>coli<\/jats:italic> bacterial counts and dissemination were not affected by training.<\/jats:p><\/jats:sec><jats:sec><jats:title>Interpretation<\/jats:title><jats:p>High\u2010intensity training induces superior effects in preventing autoimmunity in EAE, but does not alter immune responses to <jats:italic>E.<\/jats:italic>\u00a0<jats:italic>coli<\/jats:italic> infection.<\/jats:p><\/jats:sec>","DOI":"10.1002\/acn3.50859","type":"journal-article","created":{"date-parts":[[2019,8,1]],"date-time":"2019-08-01T08:39:03Z","timestamp":1564648743000},"page":"1647-1658","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Exercise intensity\u2010dependent immunomodulatory effects on 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