{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,6]],"date-time":"2026-06-06T19:56:31Z","timestamp":1780775791240,"version":"3.54.1"},"reference-count":43,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2020,8,13]],"date-time":"2020-08-13T00:00:00Z","timestamp":1597276800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Tianjin Binhai New District Social Development Science and Technology Project","award":["BHXQKJXM-SF-2018-02"],"award-info":[{"award-number":["BHXQKJXM-SF-2018-02"]}]},{"name":"Tianjin Education Commission Scientific Research Project","award":["2018KJ059"],"award-info":[{"award-number":["2018KJ059"]}]},{"name":"Tianjin Education Commission Scientific Research Project","award":["2018KJ060"],"award-info":[{"award-number":["2018KJ060"]}]},{"DOI":"10.13039\/501100001809","name":"National Nature Science Foundation of China","doi-asserted-by":"crossref","award":["81870831"],"award-info":[{"award-number":["81870831"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Ann Clin Transl Neurol"],"published-print":{"date-parts":[[2020,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>This study aimed to investigate plasma neuronally derived extracellular vesicle (NDEV) levels of core pathological markers [amyloid\u2010\u03b2 (A\u03b2) and phosphorylated tau] and inflammatory biomarkers, including interleukin 6 (IL\u20106) and matrix metalloproteinase\u20109 (MMP\u20109) in patients with Alzheimer\u2019s disease (AD).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Thirty\u2010one patients with AD and 15 cognitively normal controls (NCs) were recruited. The diagnosis of AD was supported by fluorodeoxyglucose and Pittsburgh Compound\u2010B PET scans. Plasma extracellular vesicles were extracted, precipitated, and enriched for neuronal source by anti\u2010L1CAM antibody absorption. Levels of A\u03b242, P\u2010T181\u2010tau, P\u2010S396\u2010tau, IL\u20106, and MMP\u20109 in plasma NDEVs were quantified by enzyme\u2010linked immunosorbent assay (ELISA).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>A\u03b242, P\u2010T181\u2010tau, and MMP\u20109 levels in plasma NDEVs were significantly higher in patients with AD than NCs. However, P\u2010S396\u2010tau and IL\u20106 levels in plasma NDEVs did not differ between AD patients and NCs. Moreover, there was no correlation between any of these biomarker levels and cognitive function as measured with Mini\u2010Mental State Examination in patients with AD.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>These findings provide further support that levels of core pathological markers, including A\u03b242 and P\u2010T181\u2010tau, are elevated in plasma NDEVs of patients with AD. Furthermore, MMP\u20109 might play an important role in the pathogenesis of AD, and is a promising inflammatory biomarker for AD.<\/jats:p><\/jats:sec>","DOI":"10.1002\/acn3.51155","type":"journal-article","created":{"date-parts":[[2020,8,13]],"date-time":"2020-08-13T13:53:24Z","timestamp":1597326804000},"page":"1681-1691","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":60,"title":["Elevated matrix metalloproteinase\u20109 levels in neuronal extracellular vesicles in Alzheimer\u2019s disease"],"prefix":"10.1002","volume":"7","author":[{"given":"Dongmei","family":"Gu","sequence":"first","affiliation":[{"name":"Department of Clinical Laboratory Tianjin Medical University General Hospital Tianjin China"},{"name":"Department of Clinical Laboratory Tianjin Medical University General Hospital Airport Hospital Tianjin 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