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In addition, 36 samples from 18 NMOSD, 12 MS, and 6 sex\u2010 and age\u2010matched HCs for detecting pro\u2010inflammatory cytokines (IFN\u03b3 and TNF\u03b1) using flow cytometry.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Compared with HCs, we found significantly reduced CD8<jats:sup>+<\/jats:sup> T<jats:sub>N<\/jats:sub> and increased CD8<jats:sup>+<\/jats:sup> T<jats:sub>E\/M<\/jats:sub> in both NMOSD and MS\uff0cwhile decreased CD8<jats:sup>+<\/jats:sup> T<jats:sub>MP<\/jats:sub> was only observed in NMOSD. Patients treated with immunotherapy were associated with increased CD8<jats:sup>+<\/jats:sup> T<jats:sub>N<\/jats:sub> and decreased CD8<jats:sup>+<\/jats:sup> T<jats:sub>E\/M<\/jats:sub> in NMOSD. Moreover NMOSD cohort showed significant higher proportions of IFN\u03b3<jats:sup>+<\/jats:sup>CD8<jats:sup>+<\/jats:sup> T cells and proportions of TNF\u03b1<jats:sup>+<\/jats:sup>CD8<jats:sup>+<\/jats:sup> T cells than HC and MS cohorts. On the contrary, obviously decreased IFN\u03b3 and TNF\u03b1 were found in NMOSD patients treated with immunotherapy. Furthermore, Multivariate linear regression analyses revealed that age was negatively correlated with CD8<jats:sup>+<\/jats:sup> T<jats:sub>N<\/jats:sub> and T<jats:sub>MP<\/jats:sub>, and positively associated with T<jats:sub>SLEC<\/jats:sub>; however, sex, EDSS scores and disease phase were not significantly associated with CD8<jats:sup>+<\/jats:sup> T subpopulations.<\/jats:p><\/jats:sec><jats:sec><jats:title>Interpretation<\/jats:title><jats:p>This current study provides an evidence that circulating CD8<jats:sup>+<\/jats:sup> T cell with abnormal subpopulations and increased pro\u2010inflammatory were associated with pathogenesis of autoimmune demyelinating disease of CNS, especially in NMOSD.<\/jats:p><\/jats:sec>","DOI":"10.1002\/acn3.51241","type":"journal-article","created":{"date-parts":[[2020,11,24]],"date-time":"2020-11-24T21:38:32Z","timestamp":1606253912000},"page":"43-53","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["CD8<sup>+<\/sup> T cell subpopulations and pro\u2010inflammatory cytokines in neuromyelitis optica spectrum disorder"],"prefix":"10.1002","volume":"8","author":[{"given":"Ziyan","family":"Shi","sequence":"first","affiliation":[{"name":"Department of Neurology West China Hospital Sichuan University Chengdu 610041 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuhan","family":"Qiu","sequence":"additional","affiliation":[{"name":"Department of Neurology West China Hospital Sichuan University Chengdu 610041 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