{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,24]],"date-time":"2026-03-24T12:01:18Z","timestamp":1774353678920,"version":"3.50.1"},"reference-count":30,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2023,12,19]],"date-time":"2023-12-19T00:00:00Z","timestamp":1702944000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005762","name":"National Taiwan University Hospital","doi-asserted-by":"publisher","award":["109A147"],"award-info":[{"award-number":["109A147"]}],"id":[{"id":"10.13039\/501100005762","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005762","name":"National Taiwan University Hospital","doi-asserted-by":"publisher","award":["111S0029"],"award-info":[{"award-number":["111S0029"]}],"id":[{"id":"10.13039\/501100005762","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Ann Clin Transl Neurol"],"published-print":{"date-parts":[[2024,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>F\u201018\u2010fluorothymidine (FLT) is a positron emission tomography (PET) tracer for imaging cell proliferation in vivo. We aimed to assess FLT uptake as a marker for cerebral cell proliferation in a rat model of ischemic stroke and patients with cerebral infarct, correlating with disease severity and outcomes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Cerebral FLT PET was performed in rats subjected to transient middle cerebral artery occlusion (MCAO) and patients with cerebral infarct. PET data were analyzed and expressed as average standardized uptake value ratios (SUVRs) using cerebellar cortex as reference. Infarct volume was analyzed by 2,3,5\u2010triphenyltetrazolium chloride staining in rats and by magnetic resonance imaging in patients. Neurological function was assessed using modified Neurological Severity Score (mNSS) for rats and National Institutes of Health Stroke Scale (NIHSS) for patients.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Seven days post\u2010MCAO, rats' FLT PET displayed higher SUVRs in the infarcted brain, declining gradually until Day 28. FLT\u2010binding ratio (SUVR in the infarcted brain divided by that in contralateral side) correlated positively with stroke severity (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001), and to early mNSS decline in rats with mild to moderate stroke severity (<jats:italic>p<\/jats:italic>\u2009=\u20090.031). In 13 patients with cerebral infarct, FLT PET showed high SUVR in the infarcted regions. FLT\u2010binding ratio correlated positively with infarct volume (<jats:italic>p<\/jats:italic>\u2009=\u20090.006). Age\u2010adjusted initial NIHSS (<jats:italic>p<\/jats:italic>\u2009=\u20090.035) and early NIHSS decline (<jats:italic>p<\/jats:italic>\u2009=\u20090.076) showed significance or a trend toward positive correlation with the FLT\u2010binding ratio.<\/jats:p><\/jats:sec><jats:sec><jats:title>Interpretation<\/jats:title><jats:p>In vivo FLT PET detects poststroke cerebral cell proliferation, which is associated with stroke severity and\/or outcomes in MCAO rats and patients with cerebral infarct.<\/jats:p><\/jats:sec>","DOI":"10.1002\/acn3.51972","type":"journal-article","created":{"date-parts":[[2023,12,20]],"date-time":"2023-12-20T07:17:52Z","timestamp":1703056672000},"page":"497-507","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["In vivo detection of poststroke cerebral cell proliferation in rodents and humans"],"prefix":"10.1002","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8752-0847","authenticated-orcid":false,"given":"Pu\u2010Tien","family":"Chiang","sequence":"first","affiliation":[{"name":"Graduate Institute of Clinical Medicine, College of Medicine National Taiwan University  Taipei Taiwan"},{"name":"Department of Neurology National Taiwan University Hospital Bei\u2010Hu Branch  Taipei Taiwan"},{"name":"Department of Neurology National Taiwan University Hospital  Taipei Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hsin\u2010Hsi","family":"Tsai","sequence":"additional","affiliation":[{"name":"Department of Neurology National Taiwan University Hospital Bei\u2010Hu Branch  Taipei Taiwan"},{"name":"Department of Neurology National Taiwan University Hospital  Taipei Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ruoh\u2010Fang","family":"Yen","sequence":"additional","affiliation":[{"name":"Department of Nuclear Medicine National Taiwan University Hospital  Taipei Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yi\u2010Chieh","family":"Tsai","sequence":"additional","affiliation":[{"name":"Department of Neurology National Taiwan University Hospital  Taipei Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chi\u2010Han","family":"Wu","sequence":"additional","affiliation":[{"name":"Molecular Imaging Center National Taiwan University  Taipei Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ching\u2010Hung","family":"Chiu","sequence":"additional","affiliation":[{"name":"Department of Nuclear Medicine National Taiwan University Hospital  Taipei Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Li\u2010Kai","family":"Tsai","sequence":"additional","affiliation":[{"name":"Department of Neurology National Taiwan University Hospital  Taipei Taiwan"},{"name":"Department of Neurology National Taiwan University 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