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The sampled tissues included control as well as Alzheimer's disease, corticobasal degeneration, progressive supranuclear palsy, frontotemporal degeneration with tau pathology, and chronic traumatic encephalopathy.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Only eight proteins were unique to gray matter while six were unique to white matter. Comparison of the gray and white matter proteome revealed an enrichment of microglial proteins in the white matter. Consensus WGCNA sorted over 6700 protein isoforms into 46 consensus modules across the gray and white matter proteomic networks. Consensus network modules demonstrated unique and shared disease\u2010associated microglial and endothelial protein changes. Ratio WGCNA sorted over 6500 protein ratios (white:gray) into 33 modules. Modules associated with mitochondrial proteins and processes demonstrated higher white:gray ratios in diseased tissues relative to control, driven by mitochondrial protein downregulation in gray and upregulation in white.<\/jats:p><\/jats:sec><jats:sec><jats:title>Interpretation<\/jats:title><jats:p>The dataset is a valuable resource for understanding proteomic changes in human tauopathy gray and white matter. The identification of unique and shared disease\u2010associated changes across gray and white matter emphasizes the utility of examining both tissue types. Future studies of microglial, endothelial, and mitochondrial changes in white matter may provide novel insights into tauopathy\u2010associated changes in human brain.<\/jats:p><\/jats:sec>","DOI":"10.1002\/acn3.52134","type":"journal-article","created":{"date-parts":[[2024,6,25]],"date-time":"2024-06-25T06:05:39Z","timestamp":1719295539000},"page":"2138-2152","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Proteomic networks of gray and white matter reveal tissue\u2010specific changes in human tauopathy"],"prefix":"10.1002","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6129-8267","authenticated-orcid":false,"given":"Ashlyn G.","family":"Johnson","sequence":"first","affiliation":[{"name":"Neuroscience Graduate Program, Laney Graduate School Emory University  Atlanta Georgia USA"},{"name":"Center for Neurodegenerative Disease Emory University School of Medicine  Atlanta Georgia USA"},{"name":"Department of Neurology Emory University School of Medicine  Atlanta Georgia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric B.","family":"Dammer","sequence":"additional","affiliation":[{"name":"Center for Neurodegenerative Disease Emory University School of Medicine  Atlanta Georgia USA"},{"name":"Department of Biochemistry Emory University School of Medicine  Atlanta Georgia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James A.","family":"Webster","sequence":"additional","affiliation":[{"name":"Center for Neurodegenerative Disease Emory University School of Medicine  Atlanta Georgia USA"},{"name":"Department of Neurology Emory University School of Medicine  Atlanta Georgia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Duc M.","family":"Duong","sequence":"additional","affiliation":[{"name":"Center for Neurodegenerative Disease Emory University School of Medicine  Atlanta Georgia USA"},{"name":"Department of Biochemistry Emory University School of Medicine  Atlanta Georgia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicholas T.","family":"Seyfried","sequence":"additional","affiliation":[{"name":"Center for Neurodegenerative Disease Emory University School of Medicine  Atlanta Georgia USA"},{"name":"Department of Biochemistry Emory University School of Medicine  Atlanta Georgia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chadwick M.","family":"Hales","sequence":"additional","affiliation":[{"name":"Center for Neurodegenerative Disease Emory University School of Medicine  Atlanta Georgia USA"},{"name":"Department of Neurology Emory University School of Medicine  Atlanta Georgia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,6,24]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13024\u2010021\u201000476\u2010x"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.3233\/JAD\u2010190924"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13024\u2010022\u201000533\u2010z"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41591\u2010020\u20100815\u20106"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2020.107807"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40478\u2010022\u201001387\u20108"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.201600057"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.ra118.001747"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13195\u2010022\u201001113\u20105"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1212\/WNL.0000000000207250"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40478\u2010021\u201001271\u2010x"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13024\u2010021\u201000462\u20103"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13024\u2010018\u20100282\u20104"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.3389\/fsysb.2023.1092341"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12859\u2010016\u20101323\u2010z"},{"key":"e_1_2_9_17_1","volume-title":"CellTypeFET: Calculate and Plot Significance of Gene List Overlap","author":"Dammer E","year":"2023"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkaa1011"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010019\u201008976\u20107"},{"key":"e_1_2_9_20_1","volume-title":"GOparallel: Uses Monthly Updated .GMT Formatted Ontology Gene Lists for Fisher Exact Enrichment into Differential or Co\u2010Expression or Other User\u2010Supplied Gene Lists. 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