{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T04:33:56Z","timestamp":1782966836310,"version":"3.54.5"},"reference-count":60,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2024,10,29]],"date-time":"2024-10-29T00:00:00Z","timestamp":1730160000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Ann Clin Transl Neurol"],"published-print":{"date-parts":[[2024,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>Patients with ovarian cancer (OC) may develop anti\u2010Yo\u2010associated paraneoplastic cerebellar degeneration (PCD)\u2014a cerebellar ataxia associated with tumor\u2010induced autoimmunity against CDR2 and CDR2L proteins. Dysregulation of circulating exosomal microRNAs (miRNAs) occur in OC. Here, we investigated whether PCD is associated with changes in the exosomal miRNA profiles of OC patients.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Serum exosomes were isolated from patients with OC (<jats:italic>n\u2009=\u200915<\/jats:italic>), patients with OC and anti\u2010Yo\u2010associated PCD (<jats:italic>n\u2009=\u200914<\/jats:italic>) and healthy controls (HC, <jats:italic>n\u2009=\u200915<\/jats:italic>). Small RNA sequencing was used to identify differentially expressed miRNAs. Receiver operating characteristic curves were used to evaluate biomarker sensitivity and specificity, and miRNA target prediction analysis was employed to elucidate gene targets.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>OC patients with PCD exhibited a distinct exosomal miRNA expression profile. We detected 103 differentially expressed exosomal miRNAs in PCD patients compared to OC patients without PCD and 139 differentially expressed exosomal miRNAs compared to controls. Particularly miR\u2010486\u20105p, miR\u20104732\u20105p, miR\u201098\u20105p and miR\u201021\u20105p exhibited notable sensitivity and specificity for discriminating PCD patients from both OC patients without PCD and healthy controls. miRNA target prediction showed that several of the differentially expressed miRNAs in PCD patients targeted the <jats:italic>CDR2<\/jats:italic> and <jats:italic>CDR2L<\/jats:italic> genes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Interpretation<\/jats:title><jats:p>Our results demonstrate that OC patients with anti\u2010Yo\u2010associated PCD exhibit a distinct exosomal miRNA profile compared to OC patients without PCD. Several of the differentially expressed exosomal miRNAs in PCD patients showed diagnostic potential and may hold relevance for understanding the pathogenesis of PCD.<\/jats:p><\/jats:sec>","DOI":"10.1002\/acn3.52232","type":"journal-article","created":{"date-parts":[[2024,10,30]],"date-time":"2024-10-30T09:07:21Z","timestamp":1730279241000},"page":"3255-3266","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Altered exosomal <scp>miRNA<\/scp> profiles in patients with paraneoplastic cerebellar degeneration"],"prefix":"10.1002","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9318-8388","authenticated-orcid":false,"given":"Eirik","family":"Tveit Solheim","sequence":"first","affiliation":[{"name":"Department of Clinical Medicine University of Bergen  Bergen Norway"},{"name":"Departments of Neurology and Clinical Medicine Neuro\u2010SysMed \u2010 Centre of Excellence for Experimental Therapy in Neurology 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