{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T14:01:26Z","timestamp":1790085686677,"version":"4.0.1"},"reference-count":27,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2025,2,3]],"date-time":"2025-02-03T00:00:00Z","timestamp":1738540800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000098","name":"Clinical Center","doi-asserted-by":"publisher","award":["K23NS117883"],"award-info":[{"award-number":["K23NS117883"]}],"id":[{"id":"10.13039\/100000098","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000098","name":"Clinical Center","doi-asserted-by":"publisher","award":["R01NS113828"],"award-info":[{"award-number":["R01NS113828"]}],"id":[{"id":"10.13039\/100000098","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Ann Clin Transl Neurol"],"published-print":{"date-parts":[[2025,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>To assess the real\u2010world performance of a live (LCBA) versus a fixed (FCBA) cell\u2010based assay for the detection of serum antibodies directed against myelin oligodendrocyte glycoprotein (MOG\u2010IgG) and aquaporin\u20104 (AQP4\u2010IgG).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>This was a retrospective study of patients evaluated at a single tertiary academic referral center, with serum testing performed clinically for AQP4\u2010IgG and\/or MOG\u2010IgG by FCBA and LCBA on the same day. Additionally, frozen banked sera from the same day for patients tested only by one assay were retrieved and tested by the other assay. FCBA was performed by the Johns Hopkins Immunology Laboratory using Euroimmun kits with detection by indirect immunofluorescence (FCBA\u2010IF), whereas LCBA was performed by the Mayo Clinic Neuroimmunology Laboratory with detection by flow cytometry (LCBA\u2010FACS).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Of 594 specimens with paired MOG\u2010IgG testing, 500 were negative by both assays, 33 were positive by both assays, 56 were positive exclusively by LCBA\u2010FACS, and 5 were only positive by FCBA\u2010IF. Overall, MOG\u2010IgG LCBA\u2010FACS exhibited 95.1% sensitivity and 97.7% specificity, whereas MOG\u2010IgG FCBA\u2010IF had 45.7% sensitivity and 99.8% specificity. Of 577 specimens with paired AQP4\u2010IgG testing, 503 were negative by both assays, 51 were positive by both assays, 21 were positive exclusively by LCBA\u2010FACS, and 2 were only positive by FCBA\u2010IF. Overall, AQP4\u2010IgG LCBA\u2010FACS exhibited 97.3% sensitivity and 100% specificity, whereas AQP4\u2010IgG FCBA\u2010IF had 71.6% sensitivity and 100% specificity.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Interpretation<\/jats:title>\n                    <jats:p>LCBA\u2010FACS for both MOG\u2010IgG and AQP4\u2010IgG had markedly better sensitivity than FCBA\u2010IF, with similar specificity. The use of FCBA\u2010IF may result in underrecognition of both MOG antibody\u2010associated disease (MOGAD) and AQP4\u2010IgG seropositive neuromyelitis optica spectrum disorder (NMOSD).<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/acn3.52310","type":"journal-article","created":{"date-parts":[[2025,2,4]],"date-time":"2025-02-04T02:29:57Z","timestamp":1738636197000},"page":"556-564","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":43,"title":["Real\u2010world clinical experience with serum\n                    <scp>MOG<\/scp>\n                    and\n                    <scp>AQP4<\/scp>\n                    antibody testing by live versus fixed cell\u2010based assay"],"prefix":"10.1002","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-4350-1910","authenticated-orcid":false,"given":"Yana","family":"Said","sequence":"first","affiliation":[{"name":"Department of Neurology Johns Hopkins University School of Medicine  Baltimore Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0419-233X","authenticated-orcid":false,"given":"Angeliki","family":"Filippatou","sequence":"additional","affiliation":[{"name":"Department of Neurology Johns Hopkins University School of Medicine  Baltimore Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Conlan","family":"Tran","sequence":"additional","affiliation":[{"name":"Department of Pathology Johns Hopkins University School of Medicine  Baltimore Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"LuAnn","family":"Rezavi","sequence":"additional","affiliation":[{"name":"Department of Pathology Johns Hopkins University School of Medicine  Baltimore Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kai","family":"Guo","sequence":"additional","affiliation":[{"name":"Departments of Neurology, Laboratory Medicine and Pathology, and Center for MS and Autoimmune Neurology Mayo Clinic College of Medicine  Rochester Minnesota USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew D.","family":"Smith","sequence":"additional","affiliation":[{"name":"Department of Neurology Johns Hopkins University School of Medicine  Baltimore Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasmin","family":"Resto","sequence":"additional","affiliation":[{"name":"Department of Neurology Johns Hopkins University School of Medicine  Baltimore Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John J.","family":"Chen","sequence":"additional","affiliation":[{"name":"Departments of Ophthalmology and Neurology, Center for MS and Autoimmune Neurology Mayo Clinic  Rochester Minnesota USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7776-6472","authenticated-orcid":false,"given":"Peter 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