{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,9]],"date-time":"2025-09-09T22:29:02Z","timestamp":1757456942342,"version":"3.37.3"},"reference-count":46,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2018,3,26]],"date-time":"2018-03-26T00:00:00Z","timestamp":1522022400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004587","name":"Instituto de Salud Carlos III","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002924","name":"Federaci\u00f3n Espa\u00f1ola de Enfermedades Raras","doi-asserted-by":"publisher","award":["FIS16\/00627"],"award-info":[{"award-number":["FIS16\/00627"]}],"id":[{"id":"10.13039\/501100002924","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Ann Clin Transl Neurol"],"published-print":{"date-parts":[[2018,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>Paraneoplastic neurological syndromes (<jats:styled-content style=\"fixed-case\">PNS<\/jats:styled-content>) are rare neurological disorders in which ectopic expression of neural antigens by a tumor results in an autoimmune attack against the nervous system. Onconeural antibodies not only guide <jats:styled-content style=\"fixed-case\">PNS<\/jats:styled-content> diagnosis but may also help detecting underlying malignancies. Our project aims to uncover new potential antibodies in paraneoplastic neuropathies (<jats:styled-content style=\"fixed-case\">PN<\/jats:styled-content>).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Thirty\u2010four patients fulfilling diagnostic criteria of possible (<jats:italic>n<\/jats:italic> = 9; 26.5%) and definite (<jats:italic>n<\/jats:italic> = 25; 73.5%) <jats:styled-content style=\"fixed-case\">PN<\/jats:styled-content> without onconeural antibodies and 28 healthy controls were included in our study. Sera were tested for known antibodies against neural cell adhesion molecules and screened for novel IgG and IgM reactivities against nerve components: dorsal root ganglia (<jats:styled-content style=\"fixed-case\">DRG<\/jats:styled-content>) neurons, motor neurons, and Schwann cells. Patients showing autoantibodies against any of these cell types were used for immunoprecipitation (<jats:styled-content style=\"fixed-case\">IP<\/jats:styled-content>) studies.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Overall, 9 (26.5%) patients showed significant reactivity against <jats:styled-content style=\"fixed-case\">DRG<\/jats:styled-content> neurons, motor neurons, or Schwann cells, whereas 5 (17.9%) healthy controls only showed moderate reactivity. Compared with control sera, serum samples from patients with paraneoplastic sensory\u2010motor neuropathies had a higher frequency of IgM antibodies against Schwann cells (0% vs. 40%; <jats:italic>P<\/jats:italic> = 0.0028). No novel antigens were identified from our <jats:styled-content style=\"fixed-case\">IP<\/jats:styled-content> experiments. Antibodies against the neural adhesion molecules <jats:styled-content style=\"fixed-case\">CNTN<\/jats:styled-content>1, <jats:styled-content style=\"fixed-case\">NF<\/jats:styled-content>155, <jats:styled-content style=\"fixed-case\">NF<\/jats:styled-content>140, <jats:styled-content style=\"fixed-case\">NF<\/jats:styled-content>186, <jats:styled-content style=\"fixed-case\">NCAM<\/jats:styled-content>1, L1<jats:styled-content style=\"fixed-case\">CAM<\/jats:styled-content>, and the <jats:styled-content style=\"fixed-case\">CNTN<\/jats:styled-content>1\/<jats:styled-content style=\"fixed-case\">CASPR<\/jats:styled-content>1 complex were not detected in patients with <jats:styled-content style=\"fixed-case\">PN<\/jats:styled-content>. One (2.9%) patient with <jats:styled-content style=\"fixed-case\">CIDP<\/jats:styled-content> and thymoma had <jats:styled-content style=\"fixed-case\">CASPR<\/jats:styled-content>2 antibodies.<\/jats:p><\/jats:sec><jats:sec><jats:title>Interpretation<\/jats:title><jats:p>Almost 30% of patients with <jats:styled-content style=\"fixed-case\">PN<\/jats:styled-content> harbor antibodies targeting neural structures, suggesting that novel neoplasm\u2010associated antigens remain to be discovered.<\/jats:p><\/jats:sec>","DOI":"10.1002\/acn3.554","type":"journal-article","created":{"date-parts":[[2018,3,26]],"date-time":"2018-03-26T08:22:00Z","timestamp":1522052520000},"page":"559-569","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Antibodies against cell adhesion molecules and neural structures in paraneoplastic neuropathies"],"prefix":"10.1002","volume":"5","author":[{"given":"Ana 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