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In the discovery set, 179 human mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s were assayed for profiling of five <jats:styled-content style=\"fixed-case\">OMG<\/jats:styled-content> patients and four age\u2010 and gender\u2010matched healthy controls. Based on the specific accumulation pattern of 19 mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s from the discovery set, in addition to mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s previously found elevated in generalized <jats:styled-content style=\"fixed-case\">MG<\/jats:styled-content> (<jats:styled-content style=\"fixed-case\">GMG<\/jats:styled-content>; miR\u2010150\u20105p and miR\u201030e\u20105p), 21 mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s were subsequently analyzed in a validation cohort of 83 <jats:styled-content style=\"fixed-case\">OMG<\/jats:styled-content> patients (82 immunosuppression treatment naive; 49 male) within 3 months of diagnosis and at a follow\u2010up visit (median duration 28 months from first visit).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Thirteen patients generalized 14.8 \u00b1 12.0 months after the diagnosis and the majority (85%) belonged to the late onset <jats:styled-content style=\"fixed-case\">MG<\/jats:styled-content> group. Two mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s were significantly higher in secondary <jats:styled-content style=\"fixed-case\">GMG<\/jats:styled-content> (<jats:styled-content style=\"fixed-case\">SGMG<\/jats:styled-content>) patients compared to <jats:styled-content style=\"fixed-case\">OMG<\/jats:styled-content> patients with late onset <jats:styled-content style=\"fixed-case\">MG<\/jats:styled-content>: miR\u201030e\u20105p (9.1 \u00b1 0.5 vs. 6.3 \u00b1 0.9; <jats:italic>P<\/jats:italic> &lt; 0.0001) and miR\u2010150\u20105p (7.4 \u00b1 1.1 vs. 6.4 \u00b1 1.1; <jats:italic>P<\/jats:italic> = 0.01). The sensitivity for miR\u201030e\u20105p in differentiating <jats:styled-content style=\"fixed-case\">OMG<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">SGMG<\/jats:styled-content> was 96% in all <jats:styled-content style=\"fixed-case\">OMG<\/jats:styled-content> patients and 100% in late onset <jats:styled-content style=\"fixed-case\">OMG<\/jats:styled-content> patients.<\/jats:p><\/jats:sec><jats:sec><jats:title>Interpretation<\/jats:title><jats:p>This is the first study to describe a potential predictive factor associated with the risk of generalization for patients with <jats:styled-content style=\"fixed-case\">OMG<\/jats:styled-content>. Raised levels (&gt;8) of miR\u201030e\u20105p at initial presentation in patients with ocular <jats:styled-content style=\"fixed-case\">MG<\/jats:styled-content> symptoms, give a predictive cut\u2010off for subsequent generalization of 96\u2013100%.<\/jats:p><\/jats:sec>","DOI":"10.1002\/acn3.692","type":"journal-article","created":{"date-parts":[[2019,1,25]],"date-time":"2019-01-25T05:09:54Z","timestamp":1548392994000},"page":"243-251","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":48,"title":["miR\u201030e\u20105p as predictor of generalization in ocular myasthenia gravis"],"prefix":"10.1002","volume":"6","author":[{"given":"Liis","family":"Sabre","sequence":"first","affiliation":[{"name":"Department of Neuroscience Clinical Neurophysiology Uppsala University Uppsala Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul","family":"Maddison","sequence":"additional","affiliation":[{"name":"Department of 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