{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T05:17:01Z","timestamp":1788153421047,"version":"build-2803163510"},"reference-count":21,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,8,30]],"date-time":"2026-08-30T00:00:00Z","timestamp":1788048000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,8,30]],"date-time":"2026-08-30T00:00:00Z","timestamp":1788048000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Ann Clin Transl Neurol"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Amyotrophic lateral sclerosis is an incurable neurodegenerative disease involving motor neuron degeneration and metabolic and immune dysfunction. We combined clinical data, cerebrospinal fluid biomarkers and fluorodeoxyglucose positron emission tomography with magnetic resonance imaging to investigate the role of reactive microglia in disease pathogenesis. Patients showed increased cerebrospinal fluid levels of neurofilament light chain and chitinases, along with hypermetabolism in the medulla oblongata. Chitinase levels correlated with brainstem metabolism and clinical severity. These findings suggest a link between microglial activation, brainstem hypermetabolism and disease progression in amyotrophic lateral sclerosis, supporting a central role for neuroinflammation in disease pathogenesis.<\/jats:p>","DOI":"10.1002\/acn3.70521","type":"journal-article","created":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T04:25:13Z","timestamp":1788150313000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Metabolic and Fluid Biomarkers Support Microglia Activation in Amyotrophic Lateral Sclerosis"],"prefix":"10.1002","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3343-1547","authenticated-orcid":false,"given":"Matteo","family":"Zanovello","sequence":"first","affiliation":[{"name":"Neuromuscular Unit, Department of Neurosciences University Hospital of Padova  Padova Italy"},{"name":"Department of Neuromuscular Diseases UCL Queen Square Institute of Neurology, UCL  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7470-0614","authenticated-orcid":false,"given":"Francesca","family":"Serani","sequence":"additional","affiliation":[{"name":"Nuclear Medicine Unit, Department of Medicine University Hospital of Padova  Padova Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alen","family":"Bebeti","sequence":"additional","affiliation":[{"name":"Neuromuscular Unit, Department of Neurosciences University Hospital of Padova  Padova Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Federica","family":"Paredi","sequence":"additional","affiliation":[{"name":"Neuromuscular Unit, Department of Neurosciences University Hospital of Padova  Padova Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giulia","family":"Quadrio","sequence":"additional","affiliation":[{"name":"Neuromuscular Unit, Department of Neurosciences University Hospital of Padova  Padova Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea","family":"Fortuna","sequence":"additional","affiliation":[{"name":"Neuromuscular Unit, Department of Neurosciences University Hospital of Padova  Padova Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giacomo Maria","family":"Minicuci","sequence":"additional","affiliation":[{"name":"Neuromuscular Unit, Department of Neurosciences University Hospital of Padova  Padova Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7740-4156","authenticated-orcid":false,"given":"Elena","family":"Pegoraro","sequence":"additional","affiliation":[{"name":"Neuromuscular Unit, Department of Neurosciences University Hospital of Padova  Padova Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Annachiara","family":"Cagnin","sequence":"additional","affiliation":[{"name":"Neuromuscular Unit, Department of Neurosciences University Hospital of Padova  Padova Italy"},{"name":"Padova Neuroscience Center University of Padova  Padova Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diego","family":"Cecchin","sequence":"additional","affiliation":[{"name":"Nuclear Medicine Unit, Department of Medicine University Hospital of Padova  Padova Italy"},{"name":"Padova Neuroscience Center University of Padova  Padova Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gianni","family":"Sorar\u00f9","sequence":"additional","affiliation":[{"name":"Neuromuscular Unit, Department of Neurosciences University Hospital of Padova  Padova Italy"},{"name":"Padova Neuroscience Center University of Padova  Padova Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,30]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1038\/nrn3430","article-title":"The Changing Scene of Amyotrophic Lateral Sclerosis","volume":"14","author":"Robberecht W.","year":"2013","journal-title":"Nature Reviews Neuroscience"},{"key":"e_1_2_10_3_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1371\/journal.pone.0042816","article-title":"The Cortical Signature of Amyotrophic Lateral Sclerosis","volume":"7","author":"Agosta F.","year":"2012","journal-title":"PLoS One"},{"key":"e_1_2_10_4_1","doi-asserted-by":"crossref","first-page":"2711","DOI":"10.1093\/brain\/awac394","article-title":"Neurofilament Light Chain in Drug Development for Amyotrophic Lateral Sclerosis: A Critical Appraisal","volume":"146","author":"Benatar M.","year":"2023","journal-title":"Brain"},{"key":"e_1_2_10_5_1","doi-asserted-by":"crossref","first-page":"715","DOI":"10.1007\/s00401-018-1933-9","article-title":"Inflammation in ALS\/FTD Pathogenesis","volume":"137","author":"McCauley M. 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