{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T04:40:58Z","timestamp":1792644058729,"version":"4.5.0"},"reference-count":35,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T00:00:00Z","timestamp":1792540800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T00:00:00Z","timestamp":1792540800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Ann Clin Transl Neurol"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Polycomb group factor 1 (PCGF1) and coiled\u2010coil domain\u2010containing protein 34 (CCDC34) have been implicated in tumorigenesis in multiple malignancies. However, their biological functions and prognostic significance in glioma remain poorly defined.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>RNA\u2010sequencing data from glioma cell lines with PCGF1 knockdown were obtained from the GEO database. Correlations between PCGF1 and CCDC34 expression were analyzed using datasets from TCGA, CGGA, and GEO. In addition, RT\u2010qPCR was performed to quantify PCGF1 and CCDC34 expression in glioma specimens. Kaplan\u2013Meier survival analyses were conducted to evaluate the prognostic value of CCDC34. Functional assays were performed using cell lines with PCGF1 or CCDC34 knockdown and overexpression to assess their roles in glioma progression and to elucidate their regulatory relationship. Cell proliferation and colony formation assays were carried out to evaluate tumorigenic capacity.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>CCDC34 expression was significantly downregulated in glioma cell lines following PCGF1 knockdown compared with controls. CCDC34 expression positively correlated with WHO tumor grade in glioma. Elevated CCDC34 expression was significantly associated with poorer patient outcomes. Functionally, CCDC34 expression was closely linked to enhanced cell proliferation and colony\u2010forming ability. Rescue experiments demonstrated that PCGF1 promotes glioma cell proliferation in a CCDC34\u2010dependent manner. Gene set enrichment analysis (GSEA) further revealed that both PCGF1 and CCDC34 expression were positively associated with hypoxia, coagulation, and epithelial\u2013mesenchymal transition (EMT) signaling pathways.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Our findings demonstrate that PCGF1 promotes glioma proliferation in a CCDC34\u2010dependent manner and suggest that CCDC34 may serve as a novel prognostic biomarker and potential therapeutic target in glioma.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/acn3.70559","type":"journal-article","created":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T04:23:08Z","timestamp":1792642988000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["<scp>PCGF1<\/scp>\n                    Promotes the Tumorigenesis of Malignancies Through Upregulating\n                    <scp>CCDC34<\/scp>\n                    in Glioma"],"prefix":"10.1002","author":[{"given":"Yuanbing","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Neurosurgery Third Xiangya Hospital, Central South University  Changsha Hunan China"},{"name":"Hunan Provincial University Key Laboratory of the Fundamental and Clinical Research on Neurodegenerative Diseases Changsha Medical University  Changsha Hunan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianbing","family":"Xiong","sequence":"additional","affiliation":[{"name":"Department of Emergency Xiangya Hospital, Central South University  Changsha Hunan China"},{"name":"National Clinical Research Center of Geriatric Disorders Xiangya Hospital, Central South University  Changsha Hunan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ziran","family":"Ou","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery The First Affiliated Hospital of University of South China  Hengyang Hunan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tianhao","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery Xiangya Hospital, Central South University  Changsha China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery Xiangya Hospital, Central South University  Changsha China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery Xiangya Hospital, Central South University  Changsha China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhi","family":"Tian","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery First Affiliated Hospital of Jishou University  Jishou Hunan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-4863-1971","authenticated-orcid":false,"given":"Wuyang","family":"Cao","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery Changde Hospital, Xiangya School of Medicine, Central South University (The First People's Hospital of Changde City)  Changde Hunan China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,21]]},"reference":[{"issue":"12","key":"e_1_2_12_2_1","doi-asserted-by":"crossref","first-page":"613","DOI":"10.1016\/j.cancergen.2012.10.009","article-title":"Genetics of Adult Glioma","volume":"205","author":"Goodenberger M. 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