{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,24]],"date-time":"2025-11-24T16:32:27Z","timestamp":1764001947671,"version":"build-2065373602"},"reference-count":11,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2014,5,27]],"date-time":"2014-05-27T00:00:00Z","timestamp":1401148800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arthritis Care &amp;amp; Research"],"published-print":{"date-parts":[[2014,6]]},"abstract":"<jats:sec><jats:title>Objective<\/jats:title><jats:p>The myeloid\u2010related proteins 8 and 14 (MRP\u20108\/MRP\u201014) and neutrophil\u2010derived S100A12 are biomarkers of inflammation. They can be used to determine the relapse risk in patients with juvenile idiopathic arthritis (JIA) after stopping antiinflammatory treatment. In this study, we tested the performance of different enzyme\u2010linked immunosorbent assays (ELISAs) in order to validate systems available for routine use.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>MRP\u20108\/MRP\u201014 and S100A12 serum concentrations of 188 JIA patients in remission were analyzed. Commercially available test systems were compared to experimental ELISAs established in house. The ability of the assays to identify JIA patients at risk for relapse was analyzed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>For MRP\u20108\/MRP\u201014, the PhiCal Calprotectin and B\u00fchlmann MRP8\/14 Calprotectin ELISAs revealed hazard ratios of 2.3 and 2.1, respectively. For S100A12, the CircuLex S100A12\/EN\u2010RAGE ELISA revealed a hazard ratio of 3.1. The commercial assays allowed a JIA relapse prediction that was at least comparable to the experimental ELISAs.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>For the prediction of JIA relapse after stopping medication, the biomarkers MRP\u20108\/MRP\u201014 and S100A12 can be determined by using assays that are available for routine use. The tested commercial MRP\u20108\/MRP\u201014 and S100A12 ELISAs showed a performance comparable to well\u2010established experimental ELISA protocols when assay\u2010specific cutoffs for the indication of relapse prediction are thoroughly applied.<\/jats:p><\/jats:sec>","DOI":"10.1002\/acr.22248","type":"journal-article","created":{"date-parts":[[2013,12,10]],"date-time":"2013-12-10T14:49:11Z","timestamp":1386686951000},"page":"949-955","source":"Crossref","is-referenced-by-count":49,"title":["Validation of Relapse Risk Biomarkers for Routine Use in Patients With Juvenile Idiopathic Arthritis"],"prefix":"10.1002","volume":"66","author":[{"given":"Friederike","family":"Rothmund","sequence":"first","affiliation":[{"name":"University Children's Hospital Munster Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joachim","family":"Gerss","sequence":"additional","affiliation":[{"name":"University of Munster Munster Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicolino","family":"Ruperto","sequence":"additional","affiliation":[{"name":"Istituto Giannina Gaslini, Paediatric Rheumatology International Trials Organisation Genoa Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jan","family":"D\u00e4britz","sequence":"additional","affiliation":[{"name":"University Children's Hospital Munster Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Helmut","family":"Wittkowski","sequence":"additional","affiliation":[{"name":"University Children's Hospital Munster Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael","family":"Frosch","sequence":"additional","affiliation":[{"name":"Children's Hospital Datteln Datteln Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nico M.","family":"Wulffraat","sequence":"additional","affiliation":[{"name":"Wilhelmina Children's Hospital Utrecht The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lucy R.","family":"Wedderburn","sequence":"additional","affiliation":[{"name":"Institute of Child Health UCL London UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dirk","family":"Holzinger","sequence":"additional","affiliation":[{"name":"University Children's Hospital and University of Munster Munster Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Faekah","family":"Gohar","sequence":"additional","affiliation":[{"name":"University Children's Hospital Munster Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sebastiaan J.","family":"Vastert","sequence":"additional","affiliation":[{"name":"Wilhelmina Children's Hospital Utrecht The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Riva","family":"Brik","sequence":"additional","affiliation":[{"name":"Rambam Medical Center Haifa Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chantal","family":"Job Deslandre","sequence":"additional","affiliation":[{"name":"H\u00f4pital Cochin Centre de R\u00e9f\u00e9rence National pour les Arthrites Juv\u00e9niles Paris France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jose Antonio","family":"Melo\u2010Gomes","sequence":"additional","affiliation":[{"name":"Instituto Portugues de Reumatologia Lisbon Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claudia","family":"Saad Magalh\u00e3es","sequence":"additional","affiliation":[{"name":"UNESP Botucatu Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roberto","family":"Barcellona","sequence":"additional","affiliation":[{"name":"Azienda Ospedaliera Sciacca (Ag) Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ricardo","family":"Russo","sequence":"additional","affiliation":[{"name":"Hospital de Pediatria Juan P. Garrahan Buenos Aires Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marco","family":"Gattorno","sequence":"additional","affiliation":[{"name":"Istituto Giannina Gaslini, Paediatric Rheumatology International Trials Organisation Genoa Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alberto","family":"Martini","sequence":"additional","affiliation":[{"name":"Istituto Giannina Gaslini, Paediatric Rheumatology International Trials Organisation, and Universit\u00e0 di Genova Genoa Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Johannes","family":"Roth","sequence":"additional","affiliation":[{"name":"University of Munster Munster Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dirk","family":"Foell","sequence":"additional","affiliation":[{"name":"University Children's Hospital and University of Munster Munster Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"name":"for the Paediatric Rheumatology International Trials Organisation (PRINTO)","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,5,27]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.20125"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.11177"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1136\/ard.2003.005686"},{"key":"e_1_2_7_5_1","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1016\/j.clim.2012.11.008","article-title":"Phagocyte\u2010derived S100 proteins in autoinflammation: putative role in pathogenesis and usefulness as biomarkers","volume":"14","author":"Kessel C","year":"2013","journal-title":"Clin Immunol"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1136\/gut.52.6.847"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1522-2683(20000201)21:3<665::AID-ELPS665>3.0.CO;2-U"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.14.9496"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1136\/annrheumdis-2012-201329"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2010.375"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1136\/annrheumdis-2011-200598"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0142(1950)3:1<32::AID-CNCR2820030106>3.0.CO;2-3"}],"container-title":["Arthritis Care &amp; Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Facr.22248","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/acrjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/acr.22248","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,2]],"date-time":"2025-10-02T15:21:36Z","timestamp":1759418496000},"score":1,"resource":{"primary":{"URL":"https:\/\/acrjournals.onlinelibrary.wiley.com\/doi\/10.1002\/acr.22248"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,5,27]]},"references-count":11,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2014,6]]}},"alternative-id":["10.1002\/acr.22248"],"URL":"https:\/\/doi.org\/10.1002\/acr.22248","archive":["Portico"],"relation":{},"ISSN":["2151-464X","2151-4658"],"issn-type":[{"type":"print","value":"2151-464X"},{"type":"electronic","value":"2151-4658"}],"subject":[],"published":{"date-parts":[[2014,5,27]]}}}