{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T14:12:42Z","timestamp":1785247962828,"version":"3.55.0"},"reference-count":42,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2022,4,29]],"date-time":"2022-04-29T00:00:00Z","timestamp":1651190400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["19K07431"],"award-info":[{"award-number":["19K07431"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["acrjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["ACR Open Rheumatology"],"published-print":{"date-parts":[[2022,7]]},"abstract":"<jats:sec><jats:title>Objective<\/jats:title><jats:p>CD14<jats:sup>+<\/jats:sup> dendritic\u2010shaped cells show a dendritic morphology under the electron microscopy and engage in a pseudoemperipolesis phenomenon with lymphocytes. CD90 has been used as a marker of a major subset of fibroblast\u2010like synoviocytes in rheumatoid arthritis (RA). In this study, we investigated the significance of CD90 expression in CD14<jats:sup>+<\/jats:sup> dendritic\u2010shaped cells and its correlation with RA chronic inflammation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Double immunofluorescence staining for CD14 and CD90 was performed in the collected tissues, including 12 active RA synovial tissues. The localization of CD14<jats:sup>+<\/jats:sup>CD90<jats:sup>+<\/jats:sup> cells, the percentages of CD14<jats:sup>+<\/jats:sup>CD90<jats:sup>+<\/jats:sup> cells and vascular areas, the degree of synovitis, and clinical data were investigated. Furthermore, CD14<jats:sup>+<\/jats:sup>CD90<jats:sup>+<\/jats:sup> cells analyzed by flow cytometry (CD14<jats:sup>high<\/jats:sup>CD90<jats:sup>intermediate (int)<\/jats:sup> cells) were sorted from RA synovial cells, and we examined their potential to differentiate into dendritic cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Double immunofluorescence staining showed that CD14<jats:sup>+<\/jats:sup>CD90<jats:sup>+<\/jats:sup> cells were abundant in RA synovial tissues. The percentages of CD14<jats:sup>+<\/jats:sup>CD90<jats:sup>+<\/jats:sup> cells and vascular areas correlated with some of the Krenn synovitis scores, but neither showed a strong correlation with RA disease activity parameters. Flow cytometry analysis indicated that CD14<jats:sup>high<\/jats:sup>CD90<jats:sup>int<\/jats:sup> cells were more abundant in both peripheral blood samples and synovial tissues in patients with active RA. CD14<jats:sup>high<\/jats:sup>CD90<jats:sup>int<\/jats:sup> cells were more likely to differentiate into dendritic cells in vitro.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>CD14<jats:sup>+<\/jats:sup> dendritic\u2010shaped cells expressed CD90 in the perivascular areas of RA synovial tissues. These findings suggest that CD14<jats:sup>+<\/jats:sup>CD90<jats:sup>+<\/jats:sup> dendritic\u2010shaped cells migrate from the peripheral blood to the synovial tissue, the site of inflammation, and may contribute to the chronic inflammation of RA as dendritic progenitor cells.<\/jats:p><\/jats:sec>","DOI":"10.1002\/acr2.11440","type":"journal-article","created":{"date-parts":[[2022,4,30]],"date-time":"2022-04-30T00:36:51Z","timestamp":1651279011000},"page":"603-612","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Association of CD90 Expression by CD14<sup>+<\/sup> Dendritic\u2010Shaped Cells in Rheumatoid Arthritis Synovial Tissue With Chronic Inflammation"],"prefix":"10.1002","volume":"4","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9994-8613","authenticated-orcid":false,"given":"Rie","family":"Kurose","sequence":"first","affiliation":[{"name":"Hirosaki University Graduate School of Medicine  Hirosaki 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