{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,29]],"date-time":"2026-06-29T20:17:18Z","timestamp":1782764238477,"version":"3.54.5"},"reference-count":20,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2023,1,18]],"date-time":"2023-01-18T00:00:00Z","timestamp":1674000000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"publisher","award":["JP21tm0424221"],"award-info":[{"award-number":["JP21tm0424221"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"publisher","award":["JP21zf0127004"],"award-info":[{"award-number":["JP21zf0127004"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["acrjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["ACR Open Rheumatology"],"published-print":{"date-parts":[[2023,2]]},"abstract":"<jats:sec><jats:title>Objective<\/jats:title><jats:p>Idiopathic inflammatory myopathies (IIM) demonstrate characteristic clinical phenotypes depending on the myositis\u2010specific antibody (MSAs) present. We aimed to identify common or MSA\u2010specific immunological pathways in different immune cell types from peripheral blood by transcriptome analysis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We recruited 33 patients with IIM who were separated into the following groups: 15 patients with active disease at onset and 18 with inactive disease under treatment. All patients were positive for MSAs: anti\u2013melanoma differentiation\u2010associated gene 5 (MDA5) antibody (Ab) in 10 patients, anti\u2010Mi\u20102 Ab in 7, and anti\u2010aminoacyl\u2010transfer RNA synthetase (ARS) Ab in 16. The patients were compared with 33 healthy controls. Twenty\u2010four immune cell types sorted from peripheral blood were analyzed by flow cytometry, RNA sequencing, and differentially expressed gene analysis combined with pathway analysis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The frequencies of memory B cell types were significantly decreased in active patients, and the frequency of plasmablasts was prominently increased in active patients with anti\u2010MDA5 Ab in comparison with healthy controls. The expression of type I interferon (IFN)\u2010stimulated genes of all immune cell types was increased in the active, but not inactive, patients. Endoplasmic reticulum stress\u2010related genes in all IIM memory B cells and oxidative phosphorylation\u2010related genes in inactive IIM double negative B cells were also increased, suggesting prominent B cell activation in IIM. Furthermore, active patients with anti\u2010MDA5 Ab, anti\u2010Mi\u20102 Ab, or anti\u2010ARS Ab were distinguished by IFN\u2010stimulated and oxidative phosphorylation\u2010related gene expression in plasmablasts.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Unique gene expression patterns in patients with IIM with different disease activity levels and MSA types suggest different pathophysiologies. Especially, B cells may contribute to common and MSA\u2010specific immunological pathways in IIM.<\/jats:p><\/jats:sec>","DOI":"10.1002\/acr2.11521","type":"journal-article","created":{"date-parts":[[2023,1,18]],"date-time":"2023-01-18T10:26:06Z","timestamp":1674037566000},"page":"93-102","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Transcriptome Profiling of Immune Cell Types in Peripheral Blood Reveals Common and Specific Pathways Involved in the Pathogenesis of <scp>Myositis\u2010Specific Antibody\u2010Positive<\/scp> Inflammatory Myopathies"],"prefix":"10.1002","volume":"5","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7549-6491","authenticated-orcid":false,"given":"Yusuke","family":"Sugimori","sequence":"first","affiliation":[{"name":"The University of Tokyo and Tokyo Metropolitan Bokutoh Hospital  Tokyo 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