{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,27]],"date-time":"2026-03-27T05:06:53Z","timestamp":1774588013491,"version":"3.50.1"},"reference-count":34,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2024,7,8]],"date-time":"2024-07-08T00:00:00Z","timestamp":1720396800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["acrjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["ACR Open Rheumatology"],"published-print":{"date-parts":[[2024,10]]},"abstract":"<jats:sec><jats:title>Objective<\/jats:title><jats:p>Anti\u2013histidyl\u2010transfer RNA synthetase (Jo\u20101) antibodies are associated with myositis as well as different extramuscular organ complications comprising the anti\u2010synthetase syndrome. This study aimed to clarify the relationship between anti\u2013Jo\u20101 epitope recognition patterns and specific clinical features of this syndrome.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>B cell epitope mapping was performed via enzyme\u2010linked immunosorbent assay in 180 patients who were anti\u2013Jo\u20101 antibody\u2010positive using overlapping peptides\/protein fragments spanning the amino\u2010terminal 151 amino acids of Jo\u20101 as substrate antigens. Statistical associations with clinical features were assessed through rank\u2010sum, correlation, and cluster analyses.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The level of reactivity against subfragments spanning amino acids 1\u2013151 of Jo\u20101 paralleled that of full\u2010length Jo\u20101, confirming the immunodominance of this amino\u2010terminal region. The corresponding frequencies of reactivity to peptides 1 (amino acids [aa] 1\u201321), 3 (aa 27\u201347), 4 (aa 40\u201360), 10 (aa 118\u2013138), and 11 (aa 131\u2013151) were 6.1%, 42.5%, 6.8%, 6.7%, and 20.3%. While anti\u2013full\u2010length Jo\u20101 antibodies were significantly associated with Raynaud phenomenon, anti\u2010fragment A2 (aa 1\u201360) and A3 (aa 1\u201390) antibodies were associated with proximal muscle weakness, Raynaud phenomenon, arthritis, and sicca syndrome. Anti\u2010fragment A4 (aa 1\u2013120) and A5 (aa 1\u2013151) antibodies were also associated with sicca syndrome. Peptide 1 (aa 1\u201321) antibodies were associated with Raynaud phenomenon and dysphagia. Whereas anti\u2010peptide 3 (aa 27\u201347) antibodies were also linked to Raynaud phenomenon, anti\u2010peptide 9 (aa 105\u2013125) antibodies were associated with mechanic's hands.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Autoantibodies targeting different amino\u2010terminal subfragments and\/or peptides of Jo\u20101 were associated with specific clinical features of the anti\u2010synthetase syndrome, demonstrating the biomarker potential of B cell epitope profiling in this disorder.<\/jats:p><\/jats:sec>","DOI":"10.1002\/acr2.11715","type":"journal-article","created":{"date-parts":[[2024,7,8]],"date-time":"2024-07-08T06:09:39Z","timestamp":1720418979000},"page":"615-624","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Relationship between Jo\u20101 B Cell Epitope Profile and Clinical Features of Anti\u2010Synthetase 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