{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,22]],"date-time":"2025-10-22T23:21:05Z","timestamp":1761175265295,"version":"build-2065373602"},"reference-count":54,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2025,7,29]],"date-time":"2025-07-29T00:00:00Z","timestamp":1753747200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["acrjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["ACR Open Rheumatology"],"published-print":{"date-parts":[[2025,8]]},"abstract":"<jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>To facilitate appropriate management, we aimed to evaluate associations between lymphocyte subsets and related cytokines with clinical characteristics and treatment outcomes of patients with systemic juvenile idiopathic arthritis (sJIA).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We collected blood samples from 53 patients with sJIA and evaluated the percentages of lymphocyte subsets and cytokine levels. Patients were categorized into active (n = 18), persistent (n = 11), and inactive (n = 24) sJIA groups based on clinical phenotypes. Seventeen patients with active disease provided longitudinal blood samples at 0, 4 to 6, and 24 weeks. Among these patients, 12 patients were classified as sJIA treatment responders (sJIA\u2010R) and 5 patients were classified as sJIA treatment nonresponders (sJIA\u2010NR). The healthy control (HC) group comprised 32 age\u2010matched children.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Dynamic changes in lymphocyte subsets and cytokine levels were observed over time. In the longitudinal cohort, patients in the sJIA\u2010R group showed a significant decline in proinflammatory cytokines, including interleukin\u20101 (IL\u20101), interferon\u2010\u03b3 (IFN\u2010\u03b3),\u00a0monocyte chemoattractant protein\u20101, and IL\u201023, along with increases in CD3\n                      <jats:sup>+<\/jats:sup>\n                      , CD4\n                      <jats:sup>+<\/jats:sup>\n                      , and CD8\n                      <jats:sup>+<\/jats:sup>\n                      T cells. In contrast, patients in the sJIA\u2010NR group had persistently elevated IL\u20101, IL\u201023, and tumor necrosis factor levels and natural killer (NK) T cell percentages. Despite clinical improvement, IL\u201018 and IFN\u2010\u03b3 levels remained elevated compared to HCs throughout the 24\u2010week follow\u2010up. Analysis of minor lymphocyte subsets revealed low NK and gamma delta (\u03b3\u03b4) T cell percentages during active disease and follow\u2010up. Notably, IL\u201023 levels and \u03b3\u03b4 T cell percentages were significantly associated with several disease activity parameters.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Patients with sJIA exhibit altered lymphocyte subsets and inflammatory mediator production during the disease course, which may assist in identifying treatment responders and guiding therapeutic strategies.<\/jats:p>\n                    <jats:p>\n                      <jats:boxed-text content-type=\"graphic\" position=\"anchor\">\n                        <jats:graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" mimetype=\"image\/png\" position=\"anchor\" specific-use=\"enlarged-web-image\" xlink:href=\"graphic\/acr270070-gra-0001-m.png\">\n                          <jats:alt-text>image<\/jats:alt-text>\n                        <\/jats:graphic>\n                      <\/jats:boxed-text>\n                      This study investigated associations of lymphocyte subpopulations, related cytokines, clinical phenotypes, and outcomes in systemic juvenile idiopathic (sJIA). Low percentages of NK and \u03b3\u03b4\u2010T cells were observed in patients with sJIA, particularly during active disease, compared to healthy controls. Persistent elevations in IL\u20101, IL\u201023, TNF, and NK T cell percentage were associated with poor treatment response.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/acr2.70070","type":"journal-article","created":{"date-parts":[[2025,7,29]],"date-time":"2025-07-29T10:43:51Z","timestamp":1753785831000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Associations of Lymphocyte Subpopulations, Related Cytokines, Clinical Phenotypes, and Outcomes in Systemic Juvenile Idiopathic Arthritis"],"prefix":"10.1002","volume":"7","author":[{"given":"Butsabong","family":"Lerkvaleekul","sequence":"first","affiliation":[{"name":"Faculty of Medicine Ramathibodi Hospital Mahidol University  Bangkok Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Noppasorn","family":"Sitthisarunkul","sequence":"additional","affiliation":[{"name":"Faculty of Medicine Ramathibodi Hospital 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