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VMB\u20101 exhibits 94% sequence homology with several recently reported but poorly characterized metallo\u2010\u03b2\u2010lactamases (MBLs) produced by the marine organisms <jats:italic>Alteromonadaceae<\/jats:italic>, <jats:italic>Glaciecola<\/jats:italic>, and <jats:italic>Thalassomonas actiniarum<\/jats:italic>. Sequence alignment analysis shows that VMB\u20101 shares a structurally identical active site with subclass B1 MBLs. Importantly, pVB1796 is found to be efficiently transferred from <jats:italic>Vibrio<\/jats:italic> to other Gram\u2010negative bacterial pathogens, including <jats:italic>Salmonella typhimurium<\/jats:italic>, <jats:italic>Klebsiella pneumoniae<\/jats:italic>, and <jats:italic>Acinetobacter baumanni<\/jats:italic>, via conjugation. These findings suggest that <jats:italic>bla<\/jats:italic><jats:sub>VMB\u20101<\/jats:sub>\u2010bearing plasmids have the potential to be disseminated to other Gram\u2010negative bacterial pathogens in the near future and render carbapenems useless in treatment of multidrug resistant infections.<\/jats:p>","DOI":"10.1002\/adbi.201900221","type":"journal-article","created":{"date-parts":[[2020,1,29]],"date-time":"2020-01-29T10:02:58Z","timestamp":1580292178000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Genetic and Biochemical Characterization of VMB\u20101, a Novel Metallo\u2010\u03b2\u2010Lactamase Encoded by a Conjugative, Broad\u2010Host Range IncC Plasmid from <i>Vibrio<\/i> spp"],"prefix":"10.1002","volume":"4","author":[{"given":"Zhiwei","family":"Zheng","sequence":"first","affiliation":[{"name":"Shenzhen Key Laboratory for Food Biological Safety Control Food Safety and Technology Research Centre The Hong Kong PolyU Shenzhen Research Institute  Shenzhen 518052 P. 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