{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,19]],"date-time":"2025-11-19T07:01:24Z","timestamp":1763535684531,"version":"3.41.2"},"reference-count":47,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2020,2,10]],"date-time":"2020-02-10T00:00:00Z","timestamp":1581292800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100012553","name":"Chang Gung Memorial Hospital","doi-asserted-by":"publisher","award":["CMRPG3H0261"],"award-info":[{"award-number":["CMRPG3H0261"]}],"id":[{"id":"10.13039\/100012553","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005057","name":"National Tsing Hua University","doi-asserted-by":"publisher","award":["108Q2717E1"],"award-info":[{"award-number":["108Q2717E1"]}],"id":[{"id":"10.13039\/501100005057","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004663","name":"Ministry of Science and Technology, Taiwan","doi-asserted-by":"publisher","award":["MOST 107\u20102314\u2010B\u2010182A\u2010015\u2010MY3"],"award-info":[{"award-number":["MOST 107\u20102314\u2010B\u2010182A\u2010015\u2010MY3"]}],"id":[{"id":"10.13039\/501100004663","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Adv. Biosys."],"published-print":{"date-parts":[[2020,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Islet transplantation has been demonstrated to be a promising therapy for type 1 diabetes mellitus. Although it is a minimally invasive operating procedure and provides easy access for graft monitoring, subcutaneous transplantation of the islet only has limited therapeutic outcomes, owing to the poor capacity of skin tissue to foster revascularization in a short period. Herein, 3D cell spheroids of clinically accessible umbilical cord blood mesenchymal stem cells and human umbilical vein endothelial cells are formed and employed for codelivery with \u03b2 cells subcutaneously. The 3D stem cell spheroids, which can secrete multiple proangiogenic and prosurvival growth factors, induce robust angiogenesis and prevent \u03b2 cell graft death, as indicated by the results of in vivo bioluminescent tracking and histological analysis. These experimental data highlight the efficacy of the 3D stem cell spheroids that are fabricated using translationally applicable cell types in promoting the survival and function of subcutaneously transplanted \u03b2 cells.<\/jats:p>","DOI":"10.1002\/adbi.201900254","type":"journal-article","created":{"date-parts":[[2020,2,10]],"date-time":"2020-02-10T08:05:11Z","timestamp":1581321911000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Enhancement of Subcutaneously Transplanted \u03b2 Cell Survival Using 3D Stem Cell Spheroids with Proangiogenic and Prosurvival Potential"],"prefix":"10.1002","volume":"4","author":[{"given":"Chih\u2010Ping","family":"Yu","sequence":"first","affiliation":[{"name":"Institute of Biomedical Engineering National Tsing Hua University  Hsinchu 30013 Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jyuhn\u2010Huarng","family":"Juang","sequence":"additional","affiliation":[{"name":"Division of Endocrinology and Metabolism Department of Internal Medicine Chang Gung Memorial Hospital  Taoyuan 33305 Taiwan"},{"name":"Center for Tissue Engineering Chang Gung Memorial Hospital  Taoyuan 33305 Taiwan"},{"name":"Department of Medicine College of Medicine Chang Gung University  Taoyuan 33302 Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu\u2010Jie","family":"Lin","sequence":"additional","affiliation":[{"name":"Institute of Biomedical Engineering National Tsing Hua University  Hsinchu 30013 Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ching\u2010Wen","family":"Kuo","sequence":"additional","affiliation":[{"name":"Institute of Biomedical Engineering National Tsing Hua University  Hsinchu 30013 Taiwan"},{"name":"Department of Chemical Engineering National Tsing Hua University  Hsinchu 30013 Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Li\u2010Hung","family":"Hsieh","sequence":"additional","affiliation":[{"name":"Institute of Biomedical Engineering National Tsing Hua University  Hsinchu 30013 Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1773-8450","authenticated-orcid":false,"given":"Chieh\u2010Cheng","family":"Huang","sequence":"additional","affiliation":[{"name":"Institute of Biomedical Engineering National Tsing Hua University  Hsinchu 30013 Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,2,10]]},"reference":[{"key":"e_1_2_4_1_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(06)68341-4"},{"key":"e_1_2_4_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2013.10.024"},{"key":"e_1_2_4_2_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.1700184"},{"key":"e_1_2_4_2_2","doi-asserted-by":"publisher","DOI":"10.1002\/adhm.201900423"},{"key":"e_1_2_4_3_1","doi-asserted-by":"publisher","DOI":"10.2337\/dc14-1720"},{"key":"e_1_2_4_3_2","doi-asserted-by":"publisher","DOI":"10.2337\/diacare.28.1.164"},{"key":"e_1_2_4_4_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM200007273430401"},{"key":"e_1_2_4_4_2","doi-asserted-by":"publisher","DOI":"10.2337\/dc12-0063"},{"key":"e_1_2_4_5_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM200007273430401"},{"key":"e_1_2_4_6_1","doi-asserted-by":"publisher","DOI":"10.2337\/diabetes.54.7.2060"},{"key":"e_1_2_4_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10620-013-2682-1"},{"key":"e_1_2_4_8_1","doi-asserted-by":"publisher","DOI":"10.2337\/diabetes.51.1.66"},{"key":"e_1_2_4_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(02)12020-4"},{"key":"e_1_2_4_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.3211"},{"key":"e_1_2_4_10_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1619216114"},{"key":"e_1_2_4_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11892-010-0130-6"},{"key":"e_1_2_4_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/dmrr.2463"},{"key":"e_1_2_4_12_2","doi-asserted-by":"publisher","DOI":"10.1155\/2013\/352315"},{"key":"e_1_2_4_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/adhm.201300605"},{"key":"e_1_2_4_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2015.09.009"},{"key":"e_1_2_4_14_2","doi-asserted-by":"publisher","DOI":"10.1089\/teb.2007.0150"},{"key":"e_1_2_4_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2013.09.010"},{"key":"e_1_2_4_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jacc.2006.06.073"},{"key":"e_1_2_4_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2015.09.043"},{"key":"e_1_2_4_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11095-015-1783-2"},{"key":"e_1_2_4_18_1","doi-asserted-by":"publisher","DOI":"10.3109\/14653240903555827"},{"key":"e_1_2_4_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2010.04.067"},{"key":"e_1_2_4_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02630910"},{"key":"e_1_2_4_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.copbio.2006.08.009"},{"key":"e_1_2_4_21_1","doi-asserted-by":"publisher","DOI":"10.1634\/stemcells.2008-0944"},{"key":"e_1_2_4_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2011.04.031"},{"key":"e_1_2_4_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2011.06.008"},{"key":"e_1_2_4_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12015-012-9353-z"},{"key":"e_1_2_4_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm0603-669"},{"key":"e_1_2_4_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2010.08.097"},{"key":"e_1_2_4_25_3","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2010.02.026"},{"key":"e_1_2_4_26_1","doi-asserted-by":"publisher","DOI":"10.1097\/IJG.0000000000000108"},{"key":"e_1_2_4_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2249.2006.03066.x"},{"key":"e_1_2_4_27_2","doi-asserted-by":"publisher","DOI":"10.3727\/000000007783464461"},{"key":"e_1_2_4_28_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10456-009-9146-4"},{"key":"e_1_2_4_29_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.0959-9673.2004.00396.x"},{"key":"e_1_2_4_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm0603-685"},{"key":"e_1_2_4_31_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2007-10-118273"},{"key":"e_1_2_4_32_1","doi-asserted-by":"publisher","DOI":"10.3727\/096368912X637046"},{"key":"e_1_2_4_32_2","doi-asserted-by":"publisher","DOI":"10.1002\/sctm.17-0139"},{"key":"e_1_2_4_33_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.tea.2014.0022"},{"key":"e_1_2_4_34_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.tea.2011.0391"}],"container-title":["Advanced Biosystems"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fadbi.201900254","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/adbi.201900254","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/adbi.201900254","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/adbi.201900254","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,6]],"date-time":"2023-09-06T23:16:11Z","timestamp":1694042171000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/adbi.201900254"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,2,10]]},"references-count":47,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2020,3]]}},"alternative-id":["10.1002\/adbi.201900254"],"URL":"https:\/\/doi.org\/10.1002\/adbi.201900254","archive":["Portico"],"relation":{},"ISSN":["2366-7478","2366-7478"],"issn-type":[{"type":"print","value":"2366-7478"},{"type":"electronic","value":"2366-7478"}],"subject":[],"published":{"date-parts":[[2020,2,10]]},"assertion":[{"value":"2019-10-29","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-02-10","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"1900254"}}