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Biosys."],"published-print":{"date-parts":[[2020,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Schistosomiasis is an acute and chronic disease caused by tropical parasitic worms of the genus <jats:italic>Schistosoma<\/jats:italic>, which parasitizes annually over 200 million people worldwide. Screening of antischistosomal compounds is hampered by the low throughput and potential subjectivity of the visual evaluation of the parasite phenotypes, which affects the current drug assays. Here, an impedance\u2010based platform, capable of assessing the viability of <jats:italic>Schistosoma mansoni<\/jats:italic> schistosomula exposed to drugs, is presented. This automated and parallelized platform enables unbiased and continuous measurements of dose\u2013response relationships for more than 48 h. The platform performance is established by exposure of schistosomula to three test compounds, praziquantel, oxethazaine, and mefloquine, which are known to affect the larvae phenotypes. The system is thereafter used to investigate the response of schistosomula to methiothepine, an antipsychotic compound, which causes complex drug\u2010induced effects. Continuous monitoring of the parasites reveals transient behavioral phenotypes and allows for extracting temporal characteristics of dose\u2013response curves, which are essential for selecting drugs that feature high activity and fast kinetics of action. These measurements demonstrate that impedance\u2010based detection provides a wealth of information for the in vitro characterization of candidate antischistosomals and, represents a promising tool for the identification of new lead compounds.<\/jats:p>","DOI":"10.1002\/adbi.201900304","type":"journal-article","created":{"date-parts":[[2020,6,8]],"date-time":"2020-06-08T14:35:23Z","timestamp":1591626923000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Parallelized Impedance\u2010Based Platform for Continuous Dose\u2010Response Characterization of Antischistosomal Drugs"],"prefix":"10.1002","volume":"4","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9721-3907","authenticated-orcid":false,"given":"Paolo S.","family":"Ravaynia","sequence":"first","affiliation":[{"name":"Bioengineering Laboratory Department of Biosystems Science and Engineering  ETH Z\u00fcrich, Mattenstrasse 26 Basel 4058 Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Flavio C.","family":"Lombardo","sequence":"additional","affiliation":[{"name":"Swiss Tropical and Public Health Institute Department of Medical Parasitology and Infection Biology University of Basel  Socinstrasse 57 Basel 4051 Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefan","family":"Biendl","sequence":"additional","affiliation":[{"name":"Swiss Tropical and Public Health Institute Department of Medical Parasitology and Infection Biology University of Basel  Socinstrasse 57 Basel 4051 Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthias A.","family":"Dupuch","sequence":"additional","affiliation":[{"name":"Micro and Nanosystems Department of Mechanical and Process Engineering  ETH Z\u00fcrich, Tannenstrasse 3 Zurich 8092 Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0290-3521","authenticated-orcid":false,"given":"Jennifer","family":"Keiser","sequence":"additional","affiliation":[{"name":"Swiss Tropical and Public Health Institute Department of Medical Parasitology and Infection Biology University of Basel  Socinstrasse 57 Basel 4051 Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3838-2468","authenticated-orcid":false,"given":"Andreas","family":"Hierlemann","sequence":"additional","affiliation":[{"name":"Bioengineering Laboratory Department of Biosystems Science and Engineering  ETH Z\u00fcrich, Mattenstrasse 26 Basel 4058 Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4218-0404","authenticated-orcid":false,"given":"Mario M.","family":"Modena","sequence":"additional","affiliation":[{"name":"Bioengineering Laboratory Department of Biosystems Science and Engineering  ETH Z\u00fcrich, Mattenstrasse 26 Basel 4058 Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,6,8]]},"reference":[{"key":"e_1_2_7_1_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(09)61422-7"},{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40249-018-0444-1"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41572-018-0013-8"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(06)69440-3"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(13)61949-2"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.2471\/BLT.17.198879"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molbiopara.2014.06.002"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-002-0751-z"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tiv.2018.02.012"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/pim.12037"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0000740"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41596-018-0101-y"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182009991739"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0001-37652011000200024"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.4103\/0301-4738.195590"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02382-10"},{"key":"e_1_2_7_17_1","first-page":"98","volume":"2","author":"Tritten L.","year":"2012","journal-title":"Int. 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