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Biosys."],"published-print":{"date-parts":[[2020,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The synthesis of serotonin and dopamine with purified enzymes is described. Both pathways start from an amino acid substrate and synthesize the monoamine neurotransmitter in two enzymatic steps. The enzymes human tryptophan hydroxylase isoform 2, <jats:italic>Rattus norvegicus<\/jats:italic> tyrosine hydroxylase, <jats:italic>Chlamydia pneumoniae<\/jats:italic> Cpn1046, and aromatic amino acid decarboxylase from <jats:italic>Drosophila melanogaster<\/jats:italic> are recombinantly expressed, purified, and shown to be functional in vitro. The hydroxylases efficiently convert L\u2010DOPA (L\u2010dihydroxy\u2010phenylalanine) and 5\u2010HTP (5\u2010hydroxytryptophan) from L\u2010tyrosine and L\u2010tryptophan, respectively. A single aromatic amino acid decarboxylase is capable of converting both hydroxylated intermediates into the final neurotransmitter. The platform described here may facilitate future efforts to generate medically useful artificial cells and nanofactories.<\/jats:p>","DOI":"10.1002\/adbi.202000118","type":"journal-article","created":{"date-parts":[[2020,10,27]],"date-time":"2020-10-27T06:42:17Z","timestamp":1603780937000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Cell\u2010Free Synthesis of Dopamine and Serotonin in Two Steps with Purified Enzymes"],"prefix":"10.1002","volume":"4","author":[{"given":"Alessandro","family":"Groaz","sequence":"first","affiliation":[{"name":"CIBIO University of Trento  Via Sommarive 9 Trento 38123 Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Silvia","family":"Galvan","sequence":"additional","affiliation":[{"name":"CIBIO University of Trento  Via Sommarive 9 Trento 38123 Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luca","family":"Valer","sequence":"additional","affiliation":[{"name":"CIBIO University of Trento  Via Sommarive 9 Trento 38123 Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9399-0888","authenticated-orcid":false,"given":"Daniele","family":"Rossetto","sequence":"additional","affiliation":[{"name":"CIBIO University of Trento  Via Sommarive 9 Trento 38123 Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2668-1265","authenticated-orcid":false,"given":"Filippo","family":"Benedetti","sequence":"additional","affiliation":[{"name":"CIBIO University of Trento  Via Sommarive 9 Trento 38123 Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1799-0819","authenticated-orcid":false,"given":"Graziano","family":"Guella","sequence":"additional","affiliation":[{"name":"Department of Physics University of Trento  Via Sommarive 14 Trento 38123 Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0859-1373","authenticated-orcid":false,"given":"\u00d6mer Duhan","family":"Toparlak","sequence":"additional","affiliation":[{"name":"CIBIO University of Trento  Via Sommarive 9 Trento 38123 Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2382-198X","authenticated-orcid":false,"given":"Sheref S.","family":"Mansy","sequence":"additional","affiliation":[{"name":"CIBIO University of Trento  Via Sommarive 9 Trento 38123 Italy"},{"name":"Department of Chemistry University of Alberta  11227 Saskatchewan Dr Edmonton AB T6G 2G2 Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,10,26]]},"reference":[{"key":"e_1_2_5_1_1","doi-asserted-by":"publisher","DOI":"10.1001\/archneur.61.5.641"},{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nn1964"},{"key":"e_1_2_5_3_1","doi-asserted-by":"crossref","first-page":"19","DOI":"10.2174\/1875397301004010019","volume":"4","author":"Cianchetta* G.","year":"2010","journal-title":"Curr. 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