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Biosys."],"published-print":{"date-parts":[[2020,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>While gene and cell therapies have emerged as promising treatment strategies for various neurological conditions, heavy reliance on viral vectors can hamper widespread clinical implementation. Here, the use of tissue nanotransfection as a platform nanotechnology to drive nonviral gene delivery to nerve tissue via nanochannels, in an effective, controlled, and benign manner is explored. TNT facilitates plasmid DNA delivery to the sciatic nerve of mice in a voltage\u2010dependent manner. Compared to standard bulk electroporation (BEP), impairment in toe\u2010spread and pinprick response is not caused by TNT, and has limited to no impact on electrophysiological parameters. BEP, however, induces significant nerve damage and increases macrophage immunoreactivity. TNT is subsequently used to deliver vasculogenic cell therapies to crushed nerves via delivery of reprogramming factor genes <jats:italic>Etv2<\/jats:italic>, <jats:italic>Foxc2<\/jats:italic>, and <jats:italic>Fli1<\/jats:italic> (<jats:italic>EFF<\/jats:italic>). The results indicate the TNT\u2010based delivery of <jats:italic>EFF<\/jats:italic> in a sciatic nerve crush model leads to increased vascularity, reduced macrophage infiltration, and improved recovery in electrophysiological parameters compared to crushed nerves that are TNT\u2010treated with sham\/empty plasmids. Altogether, the results indicate that TNT can be a powerful platform nanotechnology for localized nonviral gene delivery to nerve tissue, in vivo, and the deployment of reprogramming\u2010based cell therapies for nerve repair\/regeneration.<\/jats:p>","DOI":"10.1002\/adbi.202000157","type":"journal-article","created":{"date-parts":[[2020,9,17]],"date-time":"2020-09-17T06:58:45Z","timestamp":1600325925000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Nanochannel\u2010Based Poration Drives Benign and Effective Nonviral Gene Delivery to Peripheral Nerve Tissue"],"prefix":"10.1002","volume":"4","author":[{"given":"Jordan T.","family":"Moore","sequence":"first","affiliation":[{"name":"Department of Biomedical Engineering The Ohio State University  Columbus OH 43210 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher 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