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Collagen\u2013mineral arrangement has implications for bone function, aging, and disease. However, theories on collagen\u2010mineral arrangement have been confined to studies with low spatial and\/or compositional resolution resulting in an extensive debate over the location of mineral with respect to collagen. Herein, a strategy is developed to extract a single mineralized collagen fibril from bone and analyze its composition and structure atom\u2010by\u2010atom with 3D sub\u2010nanometer accuracy and compositional clarity using atom probe tomography (APT). It is shown for the first time a method to probe fibril\u2010level mineralization and collagen\u2013mineral arrangement from an in vivo system with both the spatial and compositional precision required to comment on nanoscale collagen\u2013mineral arrangement. APT of leporine bone shows distinct and helical collagen fibrils with mineralized deposits both encapsulating and incorporated into the collagenous structures. This study demonstrates a novel fibril\u2010level detection method that can be used to probe the composition of bone and contribute new insights to the structure and organization of mineralized materials such as bones and teeth.<\/jats:p>","DOI":"10.1002\/adbi.202100657","type":"journal-article","created":{"date-parts":[[2021,7,23]],"date-time":"2021-07-23T07:07:00Z","timestamp":1627024020000},"source":"Crossref","is-referenced-by-count":27,"title":["Visualization of Collagen\u2013Mineral Arrangement Using Atom Probe Tomography"],"prefix":"10.1002","volume":"5","author":[{"given":"Bryan E. 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