{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,3]],"date-time":"2025-10-03T00:52:52Z","timestamp":1759452772657,"version":"build-2065373602"},"reference-count":31,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2022,6,23]],"date-time":"2022-06-23T00:00:00Z","timestamp":1655942400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Advanced Biology"],"published-print":{"date-parts":[[2022,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The regulatory solute carrier protein, family 1, member 1 (RS1) modulates via its N\u2010terminal domain RS1\u2010reg the activity of Na<jats:sup>+<\/jats:sup>\u2013<jats:sc>d<\/jats:sc>\u2010glucose cotransporter 1 (SGLT1) and thereby the glucose uptake in the small intestine by blocking the release of SGLT1\u2010containing vesicles at the <jats:italic>trans<\/jats:italic>\u2010Golgi network (TGN). The antidiabetic activity of RS1 is mediated by ornithindecarboxylase 1 (ODC1), catalyzing the conversion of ornithine to putrescine. Putrescine can bind to a buddying protein complex for SGLT1\u2010containing vesicles at the membrane of the TGN, triggering vesicle release. In this report, a first in\u2010depth analysis of the important binding process between ODC1 and RS1\u2010reg for regulating glucose uptake in the human organism is described by comparing results from the surface\u2010based methods, \u201csurface plasmon resonance\u201d (SPR) and \u201csurface acoustic wave\u201d (SAW) with findings by isothermal titration calorimetry (ITC). In cases of SAW and SPR, three different assay surface setups are compared, resulting in small but significant differences in <jats:italic><jats:styled-content>K<\/jats:styled-content><\/jats:italic><jats:sub>D<\/jats:sub> values for different surfaces. Noteworthy, an affinity increase by the factor of about 100 for the interaction is detected and herewith described for the first time in the presence of biological membranes that may be relevant in vivo for the biological function of RS1 and future bespoken antidiabetic drug development.<\/jats:p>","DOI":"10.1002\/adbi.202101108","type":"journal-article","created":{"date-parts":[[2022,6,23]],"date-time":"2022-06-23T06:29:21Z","timestamp":1655965761000},"source":"Crossref","is-referenced-by-count":0,"title":["Evaluation of the Influence of Biosurface Design on the Interaction between the Regulatory Peptide RS1\u2010reg and ODC1 Reveals a Membrane\u2010Dependent Affinity Increase"],"prefix":"10.1002","volume":"6","author":[{"given":"Thorsten","family":"Keller","sequence":"first","affiliation":[{"name":"Department for Functional Materials in Medicine and Dentistry Pleicherwall 2 University of W\u00fcrzburg  97070 W\u00fcrzburg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hermann","family":"Koepsell","sequence":"additional","affiliation":[{"name":"Institute of Anatomy and Cell Biology University of W\u00fcrzburg  Koellikerstra\u00dfe 6 97070 W\u00fcrzburg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3167-8466","authenticated-orcid":false,"given":"J\u00fcrgen","family":"Groll","sequence":"additional","affiliation":[{"name":"Department for Functional Materials in Medicine and Dentistry Pleicherwall 2 University of W\u00fcrzburg  97070 W\u00fcrzburg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,6,23]]},"reference":[{"key":"e_1_2_8_1_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M105975200"},{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00068.2006"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00067.2006"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00232-003-0626-y"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0854.2009.00982.x"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00055.2009"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00424-014-1619-7"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.2337\/db11-1029"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.2337\/db12-0061"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M705416200"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1124\/mol.115.101162"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1124\/mol.116.104521"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddaa121"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00424-020-02439-5"},{"key":"e_1_2_8_15_1","first-page":"13","volume-title":"Poly(ethylene glycol): Chemistry and Biological Applications","author":"Zalipsky S.","year":"1997"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1021\/la010384m"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1586\/17434440.2.4.493"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.2005.11.1"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V98.4.1231"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1163\/156856295X00724"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1023\/B:PHAM.0000036909.41843.18"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/1097-4636(20011205)57:3<432::AID-JBM1186>3.0.CO;2-G"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja0318028"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature11271"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.4103\/0019-5049.144643"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.37.23418"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi026208"},{"key":"e_1_2_8_28_1","first-page":"1616","volume-title":"Molecular Biology of the Cell","author":"Alberts B.","year":"2008"},{"key":"e_1_2_8_29_1","first-page":"3048","volume-title":"Handbook of Cell Signaling","author":"Bradshaw R. 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