{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T00:09:58Z","timestamp":1788826198565,"version":"build-2803163510"},"reference-count":55,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2023,3,9]],"date-time":"2023-03-09T00:00:00Z","timestamp":1678320000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Advanced Biology"],"published-print":{"date-parts":[[2023,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Infertility affects 10\u201315% of couples, with half attributed to male factors. An improved understanding of the cell\u2010type\u2010specific dysfunction contributing to male infertility is needed to improve available therapies; however, human testicular tissues are difficult to obtain for research purposes. To overcome this, researchers have begun to use human induced pluripotent stem cells (hiPSCs) to generate various testis\u2010specific cell types in vitro. Peritubular myoid cells (PTMs) are one such testicular cell type that serves a critical role in the human testis niche but, to date, have not been derived from hiPSCs. This study set forth to generate a molecular\u2010based differentiation method for deriving PTMs from hiPSCs, mirroring in vivo patterning factors. Whole transcriptome profiling and quantitative polymerase chain reaction (qPCR) show that this differentiation method is sufficient to derive cells with PTM\u2010like transcriptomes, including upregulation of hallmark PTM functional genes, secreted growth and matrix factors, smooth muscle, integrins, receptors, and antioxidants. Hierarchical clustering shows that they acquire transcriptomes similar to primary isolated PTMs, and immunostaining shows the acquisition of a smooth muscle phenotype. Overall, these hiPSC\u2010PTMs will allow in vitro study of patient\u2010specific PTM development and function in spermatogenesis and infertility.<\/jats:p>","DOI":"10.1002\/adbi.202200322","type":"journal-article","created":{"date-parts":[[2023,3,9]],"date-time":"2023-03-09T23:35:03Z","timestamp":1678404903000},"source":"Crossref","is-referenced-by-count":9,"title":["Differentiation of Peritubular Myoid\u2010Like Cells from Human Induced Pluripotent Stem Cells"],"prefix":"10.1002","volume":"7","author":[{"given":"Meghan","family":"Robinson","sequence":"first","affiliation":[{"name":"Vancouver Prostate Centre  Vancouver BC V6H 3Z6 Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne","family":"Haegert","sequence":"additional","affiliation":[{"name":"Vancouver Prostate Centre  Vancouver BC V6H 3Z6 Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yen\u2010Yi","family":"Li","sequence":"additional","affiliation":[{"name":"Vancouver Prostate Centre  Vancouver BC V6H 3Z6 Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tunc","family":"Morova","sequence":"additional","affiliation":[{"name":"Vancouver Prostate Centre  Vancouver BC V6H 3Z6 Canada"},{"name":"Department of Urologic Sciences University of British Columbia  Vancouver BC V5Z 1M9 Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angelina Yuan Yuan","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Cell &amp; Systems Biology and Mathematics University of Toronto  Toronto ON M5S 1A1 Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luke","family":"Witherspoon","sequence":"additional","affiliation":[{"name":"Department of Urologic Sciences University of British Columbia  Vancouver BC V5Z 1M9 Canada"},{"name":"Department of Urology The Ottawa Hospital  Ottawa ON K1Y 4E9 Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Faraz","family":"Hach","sequence":"additional","affiliation":[{"name":"Vancouver Prostate Centre  Vancouver BC V6H 3Z6 Canada"},{"name":"Department of Urologic Sciences University of British Columbia  Vancouver BC V5Z 1M9 Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephanie M.","family":"Willerth","sequence":"additional","affiliation":[{"name":"Division of Medical Sciences University of Victoria  Victoria BC V8P 3E6 Canada"},{"name":"Department of Mechanical Engineering University of Victoria  Victoria BC V8P 3E6 Canada"},{"name":"School of Biomedical Engineering University of British Columbia  Vancouver BC V6T 1Z3 Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6964-7521","authenticated-orcid":false,"given":"Ryan","family":"Flannigan","sequence":"additional","affiliation":[{"name":"Vancouver Prostate Centre  Vancouver BC V6H 3Z6 Canada"},{"name":"Department of Urologic Sciences University of British Columbia  Vancouver BC V5Z 1M9 Canada"},{"name":"Department of Urology Weill Cornell Medicine  New York NY 10065 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,3,9]]},"reference":[{"key":"e_1_2_10_1_1","doi-asserted-by":"publisher","DOI":"10.5489\/cuaj.3209"},{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.2047-2927.2012.00030.x"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1530\/REP-12-0497"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.82.1.114"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1517994113"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2605.2011.01150.x"},{"key":"e_1_2_10_6_2","doi-asserted-by":"publisher","DOI":"10.1210\/endo-124-6-2711"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2007.11.019"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12015-019-09935-x"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2011.05.034"},{"key":"e_1_2_10_9_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmed.2021.644594"},{"key":"e_1_2_10_9_3","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21238893"},{"key":"e_1_2_10_9_4","doi-asserted-by":"publisher","DOI":"10.1167\/tvst.9.3.21"},{"key":"e_1_2_10_9_5","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-63151-z"},{"key":"e_1_2_10_9_6","first-page":"153","volume":"10","author":"Freedman B. 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