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Herein, a reactive cell electrospinning strategy is described using hydrazide and aldehyde\u2010functionalized poly(oligoethylene glycol methacrylate) precursor polymers that can create nanofibrous hydrogel scaffolds with controllable local cell gradients using a sequential all\u2010aqueous process that does not require additives or external energy. Cells can be encapsulated directly during the fabrication process in different layers within the scaffold, enabling localized segregation of different cell types within the structures without compromising their capacity to proliferate (\u22484\u2010fold increase in cell density over a 14 day incubation period). This sequential reactive electrospinning approach thus offers promise to generate coculture fibrous hydrogel networks in which both the nanoscale architecture and the cell distribution can be controlled, as it is essential to recreate more complex types of tissues.<\/jats:p>","DOI":"10.1002\/adbi.202300052","type":"journal-article","created":{"date-parts":[[2023,6,6]],"date-time":"2023-06-06T02:55:37Z","timestamp":1686020137000},"source":"Crossref","is-referenced-by-count":8,"title":["Multicellular Layered Nanofibrous Poly(Oligo Ethylene Glycol Methacrylate) (POEGMA)\u2010Based Hydrogel Scaffolds via Reactive Cell Electrospinning"],"prefix":"10.1002","volume":"7","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2660-5211","authenticated-orcid":false,"given":"Fei","family":"Xu","sequence":"first","affiliation":[{"name":"Department of Chemical Engineering McMaster University  1280 Main St W Hamilton ON L8S 4L8 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