{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,9]],"date-time":"2026-01-09T19:34:03Z","timestamp":1767987243825,"version":"3.49.0"},"reference-count":69,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2023,8,23]],"date-time":"2023-08-23T00:00:00Z","timestamp":1692748800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Advanced Biology"],"published-print":{"date-parts":[[2023,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The increasing importance of fungal infections has fueled the search for new beneficial alternatives substance from plant extracts. The current study investigates the antifungal and antibiofilm activity of <jats:italic>Inula viscosa<\/jats:italic> (L.) <jats:italic>Aiton<\/jats:italic> and <jats:italic>Globularia alypum<\/jats:italic> (L.) leaves extracts against <jats:italic>Candida<\/jats:italic> both in vitro and in vivo. The inhibition of planktonic and sessile <jats:italic>Candida albicans<\/jats:italic> and <jats:italic>Candida glabrata<\/jats:italic> growth using both leaf extracts are evaluated. Moreover; an in vivo infection model using <jats:italic>Galleria mellonella<\/jats:italic> larvae; infected and treated with the extracts are performed. All extracts show fungicidal activity; with a minimum fungicidal concentration (MFC) ranging from 128 to 512\u00a0\u00b5g\u00a0mL<jats:sup>\u22121<\/jats:sup> against the two selected strains of <jats:italic>Candida<\/jats:italic>. In particular, the best results are obtained with methanolic extract of <jats:italic>I. viscosa<\/jats:italic> and <jats:italic>G. alypum<\/jats:italic> with an MFC value of 128\u00a0\u00b5g\u00a0mL<jats:sup>\u22121<\/jats:sup>. The extracts are capable to prevent 90% of biofilm development at minor concentrations ranging from 100.71 \u00b1 2.49\u00a0\u00b5g\u00a0mL<jats:sup>\u22121<\/jats:sup> to 380.4 \u00b1 0.92\u00a0\u00b5g\u00a0mL<jats:sup>\u22121<\/jats:sup>. In vivo, tests on <jats:italic>Galleria mellonella<\/jats:italic> larvae show that the extracts increase the survival of the larvae infected with <jats:italic>Candida<\/jats:italic>. The attained results reveal that <jats:italic>I. viscosa<\/jats:italic> and <jats:italic>G. alypum<\/jats:italic> extracts may be considered as new antifungal agents and biofilm inhibiting agents for the pharmaceutical and agro\u2010food field.<\/jats:p>","DOI":"10.1002\/adbi.202300081","type":"journal-article","created":{"date-parts":[[2023,8,23]],"date-time":"2023-08-23T23:45:52Z","timestamp":1692834352000},"source":"Crossref","is-referenced-by-count":5,"title":["Biofilm Inhibition of <i>Inula viscosa<\/i> (L.) <i>Aiton<\/i> and <i>Globularia alypum<\/i> L. Extracts Against <i>Candida<\/i> Infectious Pathogens and In Vivo Action on <i>Galleria mellonella<\/i> Model"],"prefix":"10.1002","volume":"7","author":[{"given":"Fadoua","family":"Asraoui","sequence":"first","affiliation":[{"name":"Laboratory of Applied Biology and Pathologies Department of Biology Faculty of Sciences of Tetouan Abdelmalek Essa\u00e2di University  Tetouan 93000 Morocco"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fouad","family":"El Mansouri","sequence":"additional","affiliation":[{"name":"Research Team: Materials, Environment and Sustainable Development (MEDD) Faculty of Sciences and Techniques of Tangier Abdelmalek Essaadi University  B.P. 416 Tangier 90000 Morocco"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1296-7633","authenticated-orcid":false,"given":"Francesco","family":"Cacciola","sequence":"additional","affiliation":[{"name":"Department of Biomedical, Dental, Morphological and Functional Imaging Sciences University of Messina  Messina 98125 Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jamal","family":"Brigui","sequence":"additional","affiliation":[{"name":"Research Team: Materials, Environment and Sustainable Development (MEDD) Faculty of Sciences and Techniques of Tangier Abdelmalek Essaadi University  B.P. 416 Tangier 90000 Morocco"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adnane","family":"Louajri","sequence":"additional","affiliation":[{"name":"Laboratory of Applied Biology and Pathologies Department of Biology Faculty of Sciences of Tetouan Abdelmalek Essa\u00e2di University  Tetouan 93000 Morocco"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Giovanna","family":"Simonetti","sequence":"additional","affiliation":[{"name":"Dipartimento di Biologia Ambientale Universit\u00e0 degli Studi di Roma \u201cLa Sapienza\u201d  P.le Aldo Moro 5 Rome 00185 Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,8,23]]},"reference":[{"key":"e_1_2_9_1_1","doi-asserted-by":"publisher","DOI":"10.3390\/jof6010028"},{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.sajb.2017.11.010"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1222236"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2672.2006.03191.x"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12906-016-1316-5"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.4315\/0362-028X.JFP-19-163"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf501266s"},{"key":"e_1_2_9_8_1","volume":"10","author":"Fraternale D.","year":"2015","journal-title":"Nat. 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