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This study utilizes single\u2010cell transcriptome sequencing (scRNA\u2010seq) to characterize the mid\u2010secretory endometrium of RIF patients. Stromal fibroblast\u2010enriched and epithelium\u2010enriched populations are collected using a two\u2010step dissociation process. After quality control, 25,315 individual cells from 3 RIF patients are analyzed. The analysis identifies 12 distinct cell types, including 6 subtypes of epithelial cells. Significantly, the study reveals the replacement of glandular epithelia with MAP2K6<jats:sup>+<\/jats:sup>EPCAM<jats:sup>DIM<\/jats:sup> epithelia in the endometrial glands of RIF patients. Furthermore, the study demonstrates that endometrial gland organoids derived from RIF patients exhibit diminished responses to sex steroids compared to the controls. Single\u2010cell regulatory network inference and clustering (SCENIC) analysis identifies cell\u2010specific <jats:italic>cis<\/jats:italic>\u2010regulatory elements and constructed regulatory networks in both groups, showing alterations gene\u2010regulatory networks in RIF patients. Cell\u2010cell communication analysis distinguishes intercellular communication between the two groups, shedding light on disrupted cellular interactions associated with RIF. In summary, these findings provide valuable insights into the cellular and molecular mechanisms underlying RIF, highlighting the roles of epithelial cells in the implantation process.<\/jats:p>","DOI":"10.1002\/adbi.202300110","type":"journal-article","created":{"date-parts":[[2023,9,10]],"date-time":"2023-09-10T21:50:37Z","timestamp":1694382637000},"source":"Crossref","is-referenced-by-count":16,"title":["Single\u2010Cell RNA Transcriptome of the Human Endometrium Reveals Epithelial Characterizations Associated with Recurrent Implantation Failure"],"prefix":"10.1002","volume":"8","author":[{"given":"Hong","family":"Zhang","sequence":"first","affiliation":[{"name":"The Center for Reproductive Medicine Obstetrics and Gynecology Department The Second Affiliated Hospital of Chongqing Medical University  Chongqing 400010 P. R. 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