{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T12:20:49Z","timestamp":1792066849659,"version":"4.4.0"},"reference-count":29,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2023,10,26]],"date-time":"2023-10-26T00:00:00Z","timestamp":1698278400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001711","name":"Schweizerischer Nationalfonds zur F\u00f6rderung der Wissenschaftlichen Forschung","doi-asserted-by":"publisher","award":["169194"],"award-info":[{"award-number":["169194"]}],"id":[{"id":"10.13039\/501100001711","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004362","name":"Schweizerische Herzstiftung","doi-asserted-by":"publisher","award":["FF21017"],"award-info":[{"award-number":["FF21017"]}],"id":[{"id":"10.13039\/501100004362","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Advanced Biology"],"published-print":{"date-parts":[[2024,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>This study compares the impact of two isolation methods, ultracentrifugation (UC) and size exclusion chromatography (SEC), on small extracellular vesicles (sEVs) from primary human cardiac mesenchymal\u2010derived progenitor cells (CPCs). sEV_UC and sEV_SEC exhibit similar size, marker expression, and miRNA cargo, but sEV_UC contains notably higher total protein levels. In vitro assays show that sEV_UC, despite an equal particle count, induces more robust ERK phosphorylation, cytoprotection, and proliferation in iPS\u2010derived cardiomyocytes (iPS\u2010CMs) compared to sEV_SEC. sEV_UC also contains elevated periostin (POSTN) protein levels, resulting in enhanced focal adhesion kinase (FAK) phosphorylation in iPS\u2010CMs. Importantly, this effect persists with treatment with soluble free\u2010sEV protein fraction from SEC (Prote_SEC), indicating that free proteins like POSTN in sEV_UC enhance FAK phosphorylation. In vivo, sEV contamination with soluble proteins doesn't affect cardiac targeting or FAK phosphorylation, underscoring the intrinsic tissue targeting properties of sEV. These findings emphasize the need for standardized sEV isolation methods, as the choice of method can impact experimental outcomes, particularly in vitro.<\/jats:p>","DOI":"10.1002\/adbi.202300185","type":"journal-article","created":{"date-parts":[[2023,10,26]],"date-time":"2023-10-26T22:33:59Z","timestamp":1698359639000},"source":"Crossref","is-referenced-by-count":11,"title":["Impact of Isolation Methods on Extracellular Vesicle Functionality In Vitro and In Vivo"],"prefix":"10.1002","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7394-1173","authenticated-orcid":false,"given":"Carolina","family":"Balbi","sequence":"first","affiliation":[{"name":"Laboratories for Translational Research Ente Ospedaliero Cantonale  Bellinzona 6500 Switzerland"},{"name":"Laboratory of Cellular and Molecular Cardiology Istituto Cardiocentro Ticino Ente Ospedaliero Cantonale  Lugano 6900 Switzerland"},{"name":"Center for Molecular Cardiology University of Zurich  Schlieren Zurich 8952 Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pietro","family":"Parisse","sequence":"additional","affiliation":[{"name":"Elettra Sincrotrone Trieste  Trieste 34149 Italy"},{"name":"CNR\u2010IOM  Trieste 34149 Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hendrik","family":"Vondracek","sequence":"additional","affiliation":[{"name":"Elettra Sincrotrone Trieste  Trieste 34149 Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edoardo","family":"Lazzarini","sequence":"additional","affiliation":[{"name":"Laboratories for Translational Research Ente Ospedaliero Cantonale  Bellinzona 6500 Switzerland"},{"name":"Laboratory for Cardiovascular Theranostics Istituto Cardiocentro Ticino Ente Ospedaliero Cantonale  Lugano 6900 Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sara","family":"Bolis","sequence":"additional","affiliation":[{"name":"Laboratories for Translational Research Ente Ospedaliero Cantonale  Bellinzona 6500 Switzerland"},{"name":"Laboratory of Cellular and Molecular Cardiology Istituto Cardiocentro Ticino Ente Ospedaliero Cantonale  Lugano 6900 Switzerland"},{"name":"Laboratory for Cardiovascular Theranostics Istituto Cardiocentro Ticino Ente Ospedaliero Cantonale  Lugano 6900 Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tudor E.","family":"Fertig","sequence":"additional","affiliation":[{"name":"Victor Babes National Institute of Pathology  Bucharest 022322 Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mihaela","family":"Gherghiceanu","sequence":"additional","affiliation":[{"name":"Victor Babes National Institute of Pathology  Bucharest 022322 Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucio","family":"Barile","sequence":"additional","affiliation":[{"name":"Laboratories for Translational Research Ente Ospedaliero Cantonale  Bellinzona 6500 Switzerland"},{"name":"Laboratory for Cardiovascular Theranostics Istituto Cardiocentro Ticino Ente Ospedaliero Cantonale  Lugano 6900 Switzerland"},{"name":"Faculty of Biomedicine Universit\u00e0 della Svizzera Italiana (USI)  Lugano 6900 Switzerland"},{"name":"Institute of Life Science Scuola Superiore Sant'Anna  Pisa 56127 Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giuseppe","family":"Vassalli","sequence":"additional","affiliation":[{"name":"Laboratories for Translational Research Ente Ospedaliero Cantonale  Bellinzona 6500 Switzerland"},{"name":"Laboratory of Cellular and Molecular Cardiology Istituto Cardiocentro Ticino Ente Ospedaliero Cantonale  Lugano 6900 Switzerland"},{"name":"Center for Molecular Cardiology University of Zurich  Schlieren Zurich 8952 Switzerland"},{"name":"Faculty of Biomedicine Universit\u00e0 della Svizzera Italiana (USI)  Lugano 6900 Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,10,26]]},"reference":[{"key":"e_1_2_10_1_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2018.04.003"},{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells8070727"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.3390\/biology9090258"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.3402\/jev.v4.27066"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.3390\/biology10050376"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules27041303"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.7150\/thno.57243"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/cvr\/cvy055"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/cvr\/cvu167"},{"key":"e_1_2_10_10_1","first-page":"383","volume":"116","author":"Milano G.","year":"2020","journal-title":"Cardiovasc. Res."},{"key":"e_1_2_10_11_1","first-page":"80","volume":"2","author":"Zhao Z.","year":"2021","journal-title":"Extracell Vesicles Circ Nucleic Acids"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells9091955"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nano.2017.03.011"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.117.309417"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep33641"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2020.461773"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamcr.2019.118538"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.2478\/s11534-011-0096-2"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/bs.abl.2020.05.004"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamem.2016.12.005"},{"key":"e_1_2_10_21_1","volume":"12","author":"Balbi C.","year":"2019","journal-title":"J Vis Exp"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00249-017-1252-4"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-70245-1"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells9030724"},{"key":"e_1_2_10_25_1","first-page":"201","volume":"38","author":"Gallet R.","year":"2017","journal-title":"Eur. Heart J."},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms20030468"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.7150\/thno.74305"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1080\/20013078.2018.1560809"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-41800-2"}],"container-title":["Advanced Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/adbi.202300185","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,2]],"date-time":"2025-10-02T15:51:33Z","timestamp":1759420293000},"score":1,"resource":{"primary":{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/10.1002\/adbi.202300185"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,10,26]]},"references-count":29,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2024,2]]}},"alternative-id":["10.1002\/adbi.202300185"],"URL":"https:\/\/doi.org\/10.1002\/adbi.202300185","archive":["Portico"],"relation":{},"ISSN":["2701-0198","2701-0198"],"issn-type":[{"value":"2701-0198","type":"print"},{"value":"2701-0198","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,10,26]]},"article-number":"2300185"}}